4
1.
Setting up Z-stack:
Enable Z-Stack and the Z-Stack dialog will appear
Press “Live” and set your first and last slice
The software will suggest optimal slice number and interval which you can change manually
Activate the
‘Optimize sectioning and step’ checkbox to see slice overlapping. Here you can
again set an optimal interval to ensure ~50% overlap between sections.
If you wish to use the match pinhole function, make sure that the ‘1AU’ button in the
‘Channels’ menu is deactivated on all tracks (no blue background of button). This function will
match pinhole sizes to the optimal pinhole size of the lowest wavelength of your imaging setup
and ensure that all sections have the same thickness.
Press “Start experiment”
2.
Setting up time series:
Enable “time series”
Time series dialog will open
Set cycle number and interval time
Press “start experiment”
3.
Tile Scan and (in this mode you have: Tiles, Multipositions):
Enable “tile scan”
Tile scan dialog will open
Set desired number of horizontal and vertical tiles
You can choose different regions to scan. Choose regions and press button “+” to add it.
Press “start experiment”
Advanced Set up allows you to do preview scan of region of interest.
You can also add multipositions in this tab:
Important: to make sure that the software images the positions in the order you added them,
make sure that you DISABLE the feature “tile regions/positions” in the options menu of the
Tiles bar (see picture on the next page)
