What laser should I use?
Here are some examples of commonly used dyes and the laser lines used to excite them:
Laser
Dye
405
Dapi, Alexa 405
458
CFP
488
GFP, FITC, Alexa 488
514
YFP
561
Alexa 543, Alexa 568, Alexa 594, Rhodamine,
Mitotracker red
594
Alexa 594
633
CY5, Alexa 633, Alexa 647
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