Iron Man User Guide v. 1.4
16
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Now we need to configure the mirrors and filters appropriately
. In the light path window, you
can click on the different positions to see what available options exist in this microscope. The laser
button is self-explanatory. From there, the light goes through a (1) dichroic (which will reflect or
transmit certain wavelengths only), then to your (2) sample, back up through the (1) dichroic,
through (3, 4) additional dichroics to separate out the different wavelengths, and through (5, 6, 7)
emission filters based on your sample.
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Available choices for each position:
(1)
Dichroic (labeled based on what wavelengths it reflects; it should reflect the laser down to
the sample and transmit the emission from the sample through) – 405/488, 405/514/594,
405/488/543/633, 458, 458/514, 488/543, 488/594, NFT 80/20 (neutral density filter,
passes 80% and blocks 20%)
(2)
<Sample>
(3)
Dichroic (split emission signal from multiple fluorphores, labelled based on what
wavelengths it reflects) – none, plate, mirror, >515, >545, >595, >635 VIS, <545
(4)
Dichroic (split emission signal from multiple fluorphores, labelled based on what
wavelengths it reflects) – mirror, >490, >515, >545
(5)
Emission filter (labelled based on what it allows through; LP means long pass and it will
allow to pass anything greater than the given wavelength; BP means band pass and it will
allow to pass anything between the given wavelengths) – LP505, LP560, LP615, LP650,
BP505-530, BP505-550, BP530-560, BP560-615
(6)
Emission filter – LP420, LP475, LP505, BP420-480, BP470-500, BP505-530, BP505-570IR
(7)
Emission filter – LP420, LP475, LP505, LP530, LP560, LP615, LP650
