Image Acquisition
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Make sure that all motorized and encoded microscope components are
correctly configured in the Micro Toolbox. For the automatic application
of shading correction, the correct configuration of the available objectives
and the cameras is particularly important (please also refer to the
"MTB2004 Configuration" manual).
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Set the light intensity with which you want to work.
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To do this, set a suitable exposure time and the white balance of the
camera.
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Set Köhler illumination on the microscope (if using transmitted light) in
order to create a reproducible image-acquisition situation.
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Create a test image of a specimen with quality that is as high as possible.
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Check the quality of the Köhler setting for each objective with which you
want to generate automatic shading correction.
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Now move the sample out of the camera’s field of view and bring the
microscope clearly out of focus.
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Generate a shading reference image for each objective, one after the
other, by clicking on the
Shading Correction
button on the camera
property page
General
.
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If you are using a fully motorized microscope, it is very easy to save and
reload the configuration of the light path setting in a
hardware setting:
•
Light intensity
•
Gray density filter
•
Setting of the condenser aperture diaphragm and field diaphragm
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You can now refocus the microscope and work with the automatic
shading correction.
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M 60-3-0014 e / printed 12.2008