8
VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information
please refer to your Skyla VB1 Operator’s Manual
How to Reconstitute a Biochemistry Control Vial
Why Run a QC?
-To assess the quality and performance of the practice laboratory.
-To ensure that results generated by the Skyla VB1 are correct.
-QC monitors staff, equipment, reagents and result reporting.
Preparing the QC reagent
1. Remove vial from the refrigerator and allow to warm at room temperature for 5
minutes
2. Carefully open and remove the bung from a vial of serum and a vial of serum diluent.
3. While holding the serum vial steady on the bench, carefully pour all the diluent into
the serum vial. Do not shake excess diluent out of the diluent vial, as this has been
accounted for in the dispense.
4. Close the serum vial, swirly gently -DO NOT shake. Let the vial stand on bench for
20 minutes then swirl again and gently invert 10 times.
5. The control is now reconstituted and ready to be analysed or frozen for prolonged
storage.
QC Storage:
Store at 2-8
o
C before and after reconstitution. Reconstituted control may be stored at -20
o
C.
Thaw frozen controls quickly using warm water (37
o
C), invert gently after thawing.
QC Stability:
Open vial reconstituted stability is up to 10 days if stored at 2-8
o
C, or 2-3 weeks if stored at -
20
o
C once reconstituted. Frozen controls must be used within 8 hours of thawing.
