7
CORTECS and CORTECS Premier 2.7
µ
m Columns
[ CARE AND USE MANUAL ]
b. Storage
For periods longer than four days at room temperature, store
reversed-phase columns in 100% acetonitrile. For elevated
temperature applications, store immediately after use in 100%
acetonitrile for the best column lifetime. Do not store columns
in buffered eluents. If the mobile phase contained a buffer salt,
flush reversed-phase CORTECS Columns with 10-column
volumes of HPLC grade water (see Table 1 for common column
volumes) and replace with 100% acetonitrile for storage. Failure
to perform this intermediate step could result in precipitation
of the buffer salt in the column when 100% acetonitrile is
introduced. Completely seal column to avoid evaporation and
drying out of the bed.
For periods longer than four days, store CORTECS Columns
in 95:5 acetonitrile:water. Do not store in buffered solvent. If
the mobile phase contained a buffered salt, flush the column
with 10-column volumes of 95:5 acetonitrile:water (see Table 1
for common column volumes).
Note: If a column has been run with a mobile phase that contains
formate (e.g., ammonium formate, formic acid, etc.) and is then
flushed with 100% acetonitrile, slightly longer equilibration times
may be necessary when the column is re-installed and run again
with a formate-containing mobile phase.
V. eCORD INTELLIGENT CHIP TECHNOLOGY
(May not be available for all column configurations)
a. Introduction
The eCord Intelligent Chip provides the history of a column’s
performance throughout its lifetime. The eCord will be
permanently attached to the column to assure that the
column’s performance history is maintained in the event that
the column is moved from one instrument to another.
Figure 3. eCord Intelligent Chip.
eCord Intelligent Chip
Figure 4. eCord inserted into side of column heater.
eCord inserted into
side of column heater
At the time of manufacture, tracking, and quality control
information will be downloaded to the eCord. Storing this
information on the chip will eliminate the need for a paper
Certificate of Analysis. Once the user installs the column,
the software will automatically download key parameters
into a column history file stored on the chip. In this manual,
we explain how the eCord will provide a solution for easily
tracking the history of the columns, reduce the frustration of
paperwork trails, and give customers the reassurance that a
well-performing column is installed onto their instruments.
b. Installation
Install the column into the column heater. Plug the eCord into
the side of the column heater. Once the eCord is inserted into
the column heater the identification and overall column usage
information will be available in Empower™ and MassLynx™
Software allowing the user to access column information on
their desktop.