Waters CORTECS Care And Use Manual Download Page 3

3

CORTECS and CORTECS Premier 2.7 

µ

m Columns

[ CARE AND USE MANUAL ]

limited method flexibility other than isocratic column testing.

2.  Determine the number of theoretical plates (N) and use 

this value for periodic comparisons. 

3.  Repeat the test at predetermined intervals to track column 

performance over time. Slight variations may be observed 
due to differences in the quality of the connections, operating 
environment, system electronics, reagent quality, column 
condition, and operator technique.

e. Column QR Code

The quick reference (QR) code that is located on the column 
label provides column-specific information (i.e., the part and 
serial numbers that are unique identifiers for the column), and 
its encoding follows a widely adopted industry-standard. 

1.  Scan QR code using any device that is capable of 

scanning QR codes (i.e., for smart phones and tablets, 
use the built-in camera app).

2.  Be directed to the column’s information hub 

on waters.com.

3.  Access technical and scientific information for the column 

(i.e., certificate of analysis, application notes).

f. CORTECS Columns — VanGuard Protection

For CORTECS Columns, a separate guard holder 
and guard cartridge can be easily attached to the inlet 
of the column.  

For more information on VanGuard products, 
visit 

www.waters.com/vanguard

.

g. VanGuard FIT Cartridges 

The VanGuard FIT Cartridge is designed specifically 
for CORTECS Premier Columns. 

For the CORTECS Premier Columns that have a VanGuard FIT 
Cartridge, the VanGuard FIT Cartridge may be replaced  using 
two 3/8” wrenches. Simply apply the wrenches to the flats on 
the guard and column end nut and turn in a counter clockwise 
direction (see Figure 1). This will allow the VanGuard FIT 
Cartridge to be removed and appropriately discarded when 
following good laboratory practices.

A new VanGuard FIT Cartridge can now be used to replace 
the discarded one. Hand-tighten the new cartridge in a 
clockwise direction, then tighten using two 3/8” wrenches. 
Proper sealing should not require more than a 1/4 turn past 
the hand-tightened position.

Flats used to tighten 

guard to column

Figure 2. Recommended 3/8” wrench placement to remove the VanGuard 

FIT Cartridge from the CORTECS Premier VanGuard FIT Column.

Figure 1. Connection of the VanGuard Cartridge Column, 

VanGuard Cartridge Holder, and analytical column.

Place 3/8-inch wrench here

Place 7/16-inch wrench here

Place 5/16-inch wrench here

VanGuard 

Cartridge

VanGuard 

Cartridge Holder

HPLC Column

Summary of Contents for CORTECS

Page 1: ...CORTECS packing material is tested chromatographically with acidic basic and neutral analytes and the results are held to narrow specification ranges to assure excellent reproducible performance Ever...

Page 2: ...lumn outlet to the detector This prevents entry of air into the detection system 4 Gradually increase the flow rate as described in Step 2 5 Once a steady backpressure and baseline have been achieved...

Page 3: ...guard holder and guard cartridge can be easily attached to the inlet of the column For more information on VanGuard products visit www waters com vanguard g VanGuard FIT Cartridges The VanGuard FIT C...

Page 4: ...ating temperatures are recommended for longer column lifetime Working at high temperatures e g 45 C may also result in shorter column lifetimes III COLUMN USE To ensure the continued high performance...

Page 5: ...at 10 mM or less Ammonia NH4 OH Ammonium bicarbonate 9 2 10 3 HCO3 9 2 NH4 8 2 10 2 8 2 11 3 Volatile Volatile Yes Yes Used in the 5 10 mM range for MS work keep source 150 C Adjust pH with ammonium...

Page 6: ...rface charge modification End cap style Carbon load Ligand density pH limits Temp limits C18 Trifunctional C18 None Proprietary 6 6 2 7 mol m2 2 8 45 C C18 Trifunctional C18 Proprietary 5 7 2 4 mol m2...

Page 7: ...rmate containing mobile phase V eCORD INTELLIGENT CHIP TECHNOLOGY May not be available for all column configurations a Introduction The eCord Intelligent Chip provides the history of a column s perfor...

Page 8: ...ple set maximum pressure and temperature in the sample set and if the column met basic system suitability requirements Up to 50 sample sets can be stored on the eCord chip VI ADDITIONAL INFORMATION a...

Page 9: ...related questions to ask If I inject neat standards prepared in mobile phase do I observe these problems If I prepare my standards in water and prepare them like samples e g SPE filtration etc do I s...

Page 10: ...es are in the 0 4 to 0 8 mL min range 3 As compared to Atlantis HILIC Silica HPLC Columns the CORTECS HILIC columns are approximately 20 less retentive for gradient analysis and 35 65 less retentivefo...

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