2-16
Setting Up the Detector
Plumbing the detector
See also:
“Minimum tubing bend radius recommendations” on page C-6
.
Required Materials
•
5/16-inch open-end wrench
•
0.009-inch (0.23 mm) ID stainless steel tubing (included in the Startup
Kit)
•
Stainless steel tubing cutter or scribing file
•
Pliers, plastic-, or cloth-covered
•
Compression screw assemblies (3)
To make plumbing connections to the detector:
1. Measure the length of tubing needed to connect the column outlet to the
detector inlet.
Tip:
Keep the length of this tubing as short as possible to prevent band
broadening.
2. Measure the length of tubing needed to connect the detector outlet to a
waste collection bottle.
Tip:
Ensure the length of this tubing is at least 30 to 60 cm (1 to 2 feet)
to prevent air bubbles from forming in the flow cell.
3. Cut the two lengths of tubing as follows:
Warning:
• To avoid chemical hazards, always observe Good Laboratory
Practices when operating your system. Refer to the Material Safety
Data Sheets shipped with solvents for handling information.
• Using incompatible solvents can cause severe damage to the
instrument and injury to the operator.
Caution:
To prevent contamination, wear powder-free, nonlatex gloves
when plumbing the detector.
Caution:
To avoid damaging the flow cell, never cut the 1/32-inch
stainless steel flow cell tubing.
Summary of Contents for 2998
Page 12: ...xii Table of Contents...
Page 26: ...1 14 2998 PDA Detector Optics Principles...
Page 62: ...3 12 Maintaining the Detector...
Page 82: ...5 10 Spectral Contrast Theory...
Page 96: ...A 14 Safety Advisories...