Waters 2998, Operator'S Manual

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C-6 Solvent Considerations
Buffered solvents
When using a buffer, use a good quality reagent and filter it through a
0.45-µm filter.
Do not leave the buffer stored in the system after use. Flush all fluid line
pathways with HPLC-quality water before shutting the system down and
leave distilled water in the system (flush with 90% HPLC-quality water: 10%
methanol for shutdowns scheduled to be more than one day). Use a minimum
of 15 mL for sparge-equipped units, and a minimum of 45 mL for in-line
vacuum degasser-equipped units.
Head height
Position the solvent reservoirs at a level above the HPLC equipment or on top
of the pump or detector (with adequate spill protection).
Minimum tubing bend radius recommendations
When bending tubing, consult the following table. The bend radius on your
tubing should not be less than the arc shown in the table. The scale is 1:1 so
that the diagram can be used as a template.
Minimum bend radius for stainless steel tubing
Tubing size (OD) Minimum bend radius
1/16-inch or smaller
tubing
1/4-inch
R