Preparing for Operation
49
3
Figure 3-1 Priming the Pump
10. Turn the draw-off valve handle counterclockwise 1/2-turn to open the valve.
11. Withdraw several milliliters of eluent with the syringe, then close the valve handle.
Expel the contents of the syringe into a suitable waste container.
12. Click
Load Method
in the Inlet Method window to download the method to the
system. This will initiate the pump and flow will begin.
13. Allow the pump to run for approximately one minute, then verify normal pump
pressure for the selected eluent and flow rate. If the pump pressure is not correct,
repeat steps 2 through 6 to reset the flow rate as necessary.
14. Click the
Start/Stop Pumping
icon in the Inlet Method window to turn off the pump
flow.
3.2.2 Equilibrating the Pump
Equilibrate the system when one or more of the following conditions occur:
•
Starting the system for the first time
•
After changing eluents or adding fresh eluent
•
After leaving the system idle for more than one day
Before Equilibrating
1.
Make sure the eluent is filtered and degassed.
STOP
Attention:
To prevent eluent flow through the pump heads due to gravity pressure, never
leave the pump unattended with the draw-off valve in the open position.
Summary of Contents for 1525EF
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