UVITEC – Cambridge
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The migration curve is automatically displayed next to the lane’s profile:
The migration curve allows to check the detection of value application errors, distortion errors,
bad separation between the bands, or the quality of the standard itself.
Note: To delete the wrong data, you can either place the cursor arrow on the wrong value itself and click on it, or go for
the manual assignment.
Note: The displayed migration curve is of the cubic spline type and must then include a minimum of 4 values.
Note: The minimum MW indicates the minimum molecular weight, which can be calculated, based on the marker’s value
assignment.
Note: The maximum MW indicates the maximum molecular weight, which can be calculated, based on the marker’s
value assignment.
A
SSIGN THE MARKER VALUES TO THE BAND
Assign manually the marker values to the lane by selecting the appropriate option:
For manual assignment, click first on the molecular weight’s value to be assigned. The value is
highlighted in red. Then, click on the corresponding lane. The value is assigned to the lane: