UVITEC – Cambridge
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Note: You will be prompt to save the image prior the start of the serial acquisition.
After acquiring image N°2 the following files are created
IM0000xx_2.tif: native image acquired
IM0000xx_2Sum.tif: sum of pixel value of images xx_1 and xx_2
After acquiring image N°3
IM0000xx_3.tif : native image acquired
IM0000xx_3Sum : Sum of Im0000xx_2Sum and image Im0000xx_3
Live – Live preview pre-defined parameters
The Live folder contains the live review pre-defined set-up:
Sensitivity
This option defines the image sensitivity for your image exposure. The FireReader systems offer
exquisite resolution and sensitivity to maximize quantifiable data. The system can be used at either
its full resolution or with binning.
Sensitivity options are as followed:
The full resolution is the native camera image.
The High sensitivity and Super sensitivity modes increase the sensitivity without affecting the image
resolution using the HSR-High Sensitivity Reading technology. The High sensitivity mode combined
the HSR-High Sensitivity Reading technology and binning 2x2.
The binning technique combines the charge from adjacent pixels so that the total charge can be
read-out as a single pixel. The result is an increased signal and thus an improved sensitivity and a
better signal-to-noise ratio. This allows reducing the exposure time. The reduction of the amount of
pixels improved the frame rate of the image acquisition. However, the image resolution is decreased
by the binning factor (i.e: 4 for a binning of 2 by 2).
A 2x2 binning factor means that pixels in two rows and two columns (a total of 4 pixels) are
combined to be represented as one pixel. The sensitivity is heightened but the resolution is then
divided by 4.
