UVITEC – Cambridge
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dynamic on your image compared to the potential image
depth.
Optimum display (for 12, 14 and 16-bit image file)
The optimum display window is helpful to modify the
greyscale selection to enhance the image display.
Some images has a 12, 14 or 16-bit format and
Windows® can only display 8-bit images (256 grey
levels). Due to this limitation, the FireReader 1D software
handles two images:
A “memory” image corresponding to the 12, 14 or 16-bit
format (4 096, 16 384 or 65 536 grey levels)
A “display image” corresponding to the image displayed
on the screen (256 grey levels)
The easiest way to calculate the “display image” would be
to translate the full grey scale each time an image is
acquired: the x grey levels values of the “memory” image
corresponds to 256 values in the displayed image. In that
case, it won’t be possible to visualise faint spots on a dark
image.
FireReader 1D offers the possibility to select the grey
level range to translate for the display image calculation.
All the grey levels under the “Min value” defined will be
converted to 0 (Black) in the displayed image. All the grey
levels upper the “Max Value” defined will be set to 255
(White) in the displayed image. The grey levels between
those two limits will be converted in an intermediate grey
level value following a linear rule.
For both values, you can:
•
Edit the value in the corresponding field
•
Select the value by dragging and dropping the arrow
•
Click on the “optimum display” button: FireReader 1D will
then calculate the ideal values to be selected according to
the parameters defined
Default optimum display
Optimum display
enhancement
The image appears
brighter. The faint bands
are more visible.
Note: The optimum display has no impact on the
analysis. Only the display of the image is modified.
