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8) Click the Calibration tab. The DataBank
’s display should show Low, Med,
or High in the top right hand corner.
Note: I
f a lower case “s” is displayed followed by 4 numbers then
repeat step 7.
9)
Place the sensor in your blank solution and click “OK – proceed to step
2”.
10) The Software will start measuring the blank signal for each gain.
11) When blanking has finished clic
k “OK – proceed to step 3”.
12) From the pull down menu choose RFUB (Raw Fluorescence Units
Blanked) and click “Finalize Calculations”.
13) The following screen will appear when finalizing calculations has
completed.
14) Click OK.
15) The DataBank is now ready for use and you may begin measuring
samples. Values are reported as Blank Corrected Raw Fluorescence
Units (RFUB).
Two point Calibration (Direct Concentration)
1) Follow steps 1-11 from Single Point Calibration Section.
2) Put your sensor in a standard solution of a known concentration.
3) Enter the concentration value of your standard solution in the Enter
Standard Value box.
4) From the pull down menu choose units of measure or manually enter
units of choice; maximum 4 characters.
NOTE: DO NOT CHOOSE RFUB.
5)
Click “Proceed to step 4”.
6)
The Software will start measuring the standard solution’s signal.
7)
When completed, click “Finalize Calculations”.
8) Click OK.
9) The DataBank is now ready for use and you may begin measuring
samples. Values will be reported in units that were set in Step #4.
3.7.2 Advanced Calibration
Advanced calibration allows users to calibrate sensors using multiple
standard solutions for increased accuracy of measurement. Users can take
