998-2900
Rev L
18
3.7 Calibration and Auto Gain
This section of the manual explains the features and functions found in the
calibration tab, gives a brief overview of quick calibrations and advanced calibrations,
and explains how to configure auto gain depending on the type of sensor you’re
using. There are numerous ways to calibrate sensors using DataBank software, so it
is up to the user to determine which calibration method should be used for their
sampling protocol. Additional document for step by step calibration can be found
online:
http://docs.turnerdesigns.com/t2/doc/tech-notes/S-0173.pdf
3.7.1 Quick Calibration
Quick Calibrations can consist of a single point calibration or a two point
calibration. The DataBank will automatically generate calibration values and
formulas based on the fluorescence readings from the calibration solution
used.
A single point calibration simply requires measuring a blank solution and will
display values in (RFUB) Blank Corrected Raw Fluorescence Values.
Note: A blank solution is a solution without the fluorophore of interest,
for example, de-ionized water, ultrapure water, or filtered water
.
A two point calibration requires measuring a blank solution and a standard
solution and will display blank corrected concentration values (i.e. µg/L, ppb,
ppm, mg/L, or NTU).
Single Point Calibration (Raw Fluorescence)
1)
Select the parameter group you wish to calibrate using the DataBank’s
Select button.
2) Connect the DataBank to a PC that has DataBank Software using the
interface cable provided.
3) Begin DataBank Software and wait for a connection.
4) Once a connection has been established click the Calibration tab in the
DataBank Software.
5) Choose the parameter group you wish to calibrate from the pull down
menu.
Note: Users should choose the same parameter group as in step 1.
6) Type into the available box the name of the parameter group and hit
Enter on the keyboard
.
Note: Up to 16 characters may be entered for a name.
Note: If the name of the parameter shows “UnCalibrated” after
calibration you will need to repeat step 6 above and be sure to hit
Enter on the keyboard.
7) Click the Auto Gain tab and set the gain values using the Default buttons.
Note: Users may manually enter gain settings depending on the
sensor type.
