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Chapter 6: Operation

Thermo Scientific Wellwash

4 Mk 2 User Manual Rev.2.3, Cat. no. 1507340

Operation

6.1

Operational checklist

Table 6.1 Operational checklist

Tick

Item

Fill the wash bottle with appropriate washing reagent.

Select the program card and insert it.

When using the programmable switch card, check the settings.

Switch the instrument ON.

Prime the instrument.

Place a microplate onto the plate carrier.

Select the row number of the last row to be washed.

Start the wash sequence.

When the wash sequence has finished, remove the microplate.

Shut down the instrument.

The following symbols are used in the flowchart below:

Press the key.

Indicates the actions you should take.

key

Perform

action

Summary of Contents for Wellwash 4

Page 1: ...SERVICE MANUAL Wellwash 4 4Mk2 Thermo Fisher Scientific Ratastie 2 P O Box 100 01621 Vantaa Finland Telefax 358 9 32910 409 Tel 358 9 329 100 WW004 853 17 10 2007...

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Page 8: ...ressure on Vessel 7...

Page 9: ...imensisning o f t e elcctm icdlnsulatio nners 1e Allen K...

Page 10: ...nor its agents can b e carried out by any suita...

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Page 12: ...arrier onto the stainless steel sli the last row to ive...

Page 13: ...initiates the was icates a vacuuln uttons is confirmed...

Page 14: ...fill rows sequentially once only wi fill rows 3 times fo ollowe 2 Panel the sow number of t then rows 4 to 4...

Page 15: ...e last wasl ing cycle oft1...

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Page 17: ...ocedure i s requir Disinfection is not 1 g of the instrument...

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Page 19: ...AZARD la user protectio 110 ns are given for ad tial leak ill the pressu ion Although this occura is very rare our c a socket outlet isconlnecting the mai...

Page 20: ...rotedon has been im e ainst any unintende...

Page 21: ...roblems refer to as often as necessa...

Page 22: ...nit witc Air leaks...

Page 23: ...ubing leaks e attention or if re contact...

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Page 25: ...Wellwash Rev 2 3 Cat no 1507340 Thermo Scientific Wellwash 4 Mk 2 User Manual...

Page 26: ...eserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product develo...

Page 27: ...9 4 Functional Description 11 5 Installation 13 6 Operation 26 7 Maintenance 36 8 Troubleshooting 49 9 Technical Specifications 55 10 Ordering Information 57 11 Warranty Certificate 58 12 Index 59 13...

Page 28: ...6 Technical prerequisites 16 5 3 Installation procedure 17 5 3 1 How to install the pump unit and washer unit connections 19 5 3 2 Wash head height 23 5 3 3 Mains supply cable 24 5 3 4 Changing wash h...

Page 29: ...ical 52 8 3 3 Mechanical and environmental 53 8 4 Defects and abnormal stresses 54 8 5 Before operation 54 8 5 1 Installation 54 8 5 2 In operation 54 8 6 Cleanliness 54 9 Technical Specifications 55...

Page 30: ...the pressure from the wash bottle 30 Fig 6 2 Preprogrammed card three washes with a 12 way wash head 31 Fig 6 3 Programmable switch card 32 Fig 7 1 Liquid level sensor and liquid trap bottle cover 38...

Page 31: ...onsult Instructions for Use IVD In Vitro Diagnostic Medical Device WEEE symbol This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2002 96...

Page 32: ...Manual has been designed to give you the information you need to Review safety precautions Install the Wellwash 4 Mk 2 Carry out washing procedures Perform basic maintenance procedures Troubleshoot t...

Page 33: ...med wash card 1 Liquid dispensing into wells microplate or microstrip 2 Liquid aspiration from wells microplate or microstrip With their unique and proven design the coaxial wash heads of the Wellwash...

Page 34: ...pter 3 Introduction to the Wellwash 4 Mk 2 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 10 Quick release wash waste bottle caps Automatic pump shutdown Effective liquid managem...

Page 35: ...sed under the keypad The wash head is located above the plate carrier slideway which can be either 8 or 12 way The plate carrier moves the microplate accurately beneath the wash head and incorporates...

Page 36: ...e but the instrument remains ready for use ROW SELECT 1 12 push buttons These are momentary action switches that select the last row to be washed in any chosen program sequence An audible tone will so...

Page 37: ...nstall the wash head Refer to 5 3 steps 5 and 6 Connect the tubing and the liquid level sensor cord to the pump unit Refer to 5 3 1 steps 1 to 5 Connect the washer unit to the pump unit Refer to 5 3 1...

Page 38: ...se remember to remove the transit screws at the bottom of the instrument and save them in the adjoining screw holes marked with the text Transit screw storage Remove and save the pinch valve holder un...

Page 39: ...any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrum...

Page 40: ...nto account when deciding where the instrument should be located and operated The Wellwash 4 Mk 2 does not produce operating noise at a level that would be harmful No sound level measurements are requ...

Page 41: ...ws are refitted Failure to do so could invalidate the warranty 2 Ensure that the pump mains switch is in the OFF position After checking that the electrical supply conforms to that specified on the vo...

Page 42: ...Remove the pinch valve holder Fig 5 5 before use Store the wire holder for future use Fig 5 5 Pinch valve holder Note The wash heads are packed separately Install them before use 5 Install first the...

Page 43: ...washer unit connections Fig 5 8 Pump unit with tubes installed 1 The wash bottle with the black connections is located on the right hand side of the pump unit and the waste bottle to the left All the...

Page 44: ...r does not function if the connector is not properly inserted the black connector has to be pushed down all the way The waste bottle may otherwise overfill and cause the liquid trap bottle to start fi...

Page 45: ...he locating dowel and electrical connectors are firmly engaged Fig 5 11 1 Silicone tube waste blue color code 2 Manifold pipes PRESSURE for reagent tube yellow VACUUM for waste tube blue 3 Locating pi...

Page 46: ...h wash reagent and reconnect ensuring that the plug mates correctly with the bottle insert Fig 5 12 and that the cap ring is tightly screwed down Fig 5 12 Opening and closing the bottle 9 Tighten the...

Page 47: ...e wash head is now in the raised position 2 A stainless steel head lift rod is located on the left hand side of the washer unit immediately above the keypad There is a domed nut at the top of the rod...

Page 48: ...rs the end caps Check that the two O rings in each end cap are in position and undamaged To install the alternative wash head slide it upwards onto the slots making sure that it is fully engaged Fig 5...

Page 49: ...nds of priming the pump will automatically switch OFF The instrument will then enter its idle state waiting for instructions from the keypad and program card 7 Place a microplate onto the plate carrie...

Page 50: ...rd and insert it When using the programmable switch card check the settings Switch the instrument ON Prime the instrument Place a microplate onto the plate carrier Select the row number of the last ro...

Page 51: ...ete when the PRIME indicator turns OFF If no further key entry is made within fifteen seconds of priming then the pump will automatically switch OFF The instrument will then enter the idle state waiti...

Page 52: ...ume hood Warning When washing microplates with samples of human or animal origin keep yourself at a safe distance from the instrument while it is operating to avoid exposure Place the instrument into...

Page 53: ...th the priming reservoir to the rear under the wash head see Fig 5 13 Press the PRIME button The wash head will lift and the pinch valve will open for one second allowing wash fluid to flow into the p...

Page 54: ...e Fig 6 1 Let the hissing sound end prior to opening the cap all the way Fig 6 1 Releasing the pressure from the wash bottle Also when you are changing the used reagent and you wish to rinse and clean...

Page 55: ...unding the audible warning device 3 times Note 2 If a run is started with the rows selected but with no program card inserted the instrument aspirates the rows selected and returns to its idle state w...

Page 56: ...uring the PAUSE setting and the following wash cycle is started STRIP Each row is washed the set number of times WASHES The PAUSE setting is between each row STEPOFF The plate carrier will only pass o...

Page 57: ...ash Settings PLATE WET No aspiration wells filled PLATE DRY All rows aspirated after the final wash STRIP WET No aspiration wells filled STRIP DRY Each row aspirated after its final wash 6 5 2 Rotary...

Page 58: ...seconds 0 5 10 15 20 25 30 40 Setting 8 9 A B C D E F Strip wash mode seconds 8 9 10 15 20 30 45 60 Plate wash mode seconds 50 60 75 85 105 120 180 240 WASHES Set the number of washes to be performed...

Page 59: ...e Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled aqua a mild detergent SDS sodium dodecyl sulfate or soap solution If you have spilt infectious agent...

Page 60: ...with mild detergent followed by deionized distilled aqua See Sections 7 1 3 and 7 1 4 Check the condition of the tubing and the wash and waste bottles See Sections 7 1 5 and 7 1 6 Check the pinch val...

Page 61: ...4 Immediate Although the Wellwash 4 Mk 2 is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to...

Page 62: ...sh bottle is filled Do not let the liquid level exceed the level mark on the bottle The waste bottle is fitted with a liquid level sensor The sensor prevents the use of the instrument after the waste...

Page 63: ...Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 39 3 Release the cover by slightly lifting the tube and slide the cover then upwards along the tube Fig 7 2 Fig 7 2 Opening the liq...

Page 64: ...1507340 40 4 Pull the thick vacuum tube with the 90 angle out of the liquid trap bottle Fig 7 3 Pulling the thick tube out of the liquid trap bottle 5 Pull the thin tube out of the liquid trap bottle...

Page 65: ...side the bottle than the thin tube will be Put the bottle back to its place but don t push it yet all the way down 8 Insert the thin tube back through the liquid trap bottle cap so that the mark on th...

Page 66: ...hout first releasing the pressure by slightly opening the wash bottle cap Let the hissing sound end prior to pushing the valve top Fig 7 6 Unpinching the tube If the washer is run now the liquid will...

Page 67: ...tips The dispense aspirate tips of the wash head may become clogged with salt deposits To clear them switch OFF the washer lift up the wash arm assembly and unclog the tips using the small de clogging...

Page 68: ...sure tubing without first releasing the pressure by slightly opening the wash bottle cap Let the hissing sound end prior to disconnecting the tubing 3 Locate the fuse holders at the rear of the washer...

Page 69: ...pen Fig 7 10 Opening the fuse holder of the pump unit 9 There are two fuses inside the holder The outer one is a spare fuse and the inner one is the one in use Fig 7 11 Pump unit fuse holder opened 10...

Page 70: ...ntamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended that the complete decontaminat...

Page 71: ...l 15 Take the plate carrier and the wash head out of the glutaraldehyde solution and rinse with distilled water Reposition them on the unit 16 Flush the fluid path of the instrument with deionized dis...

Page 72: ...ions 6 Put the rest of the items user manual labels notice packing list etc into a Minigrip plastic bag and place it into its own position on the top part of the package shock absorber 7 Close and sea...

Page 73: ...ent if it appears that it does not function properly 8 1 Troubleshooting guide The problems covered below in Table 8 1 are considered as faults that require repair or corrective repair If the installa...

Page 74: ...valve and pull the tubing through the valve and reform 7 1 5 Air leaks at the wash bottle cap Tighten cap replace gasket if necessary 4 No delivery of wash reagent into wells Tubing leaks Check the tu...

Page 75: ...rty Clean the program card 11 Wash cycle does not correspond to the program card Program card is damaged Replace the program card Contact your local Thermo Fisher Scientific representative 12 Instrume...

Page 76: ...ss of service and provide you with any further details you might need e g on maintenance serviceability troubleshooting and replacement 8 3 Limitations warnings and cautions This instrument is designe...

Page 77: ...off operation and should be used as such Never reach into the workspace while the instrument is performing a wash cycle If it is necessary to stop the operation of the equipment press the RESET butto...

Page 78: ...obviate leaks Fig 5 11 Always ensure that the electrical supply in the laboratory conforms to that specified on the voltage label on the rear panel of the pump unit 8 5 2 In operation Ensure that the...

Page 79: ...se Operating range 5 C to 40 C Full specification range 15 C to 25 C Storage temperature range 10 C to 70 C packed in transport packaging Transport temperature range 40 C to 70 C packed in transport p...

Page 80: ...k 2 bears the following markings Type 006 100 120 Vac 200 240 Vac 50 60 Hz 300 VA CE mark Wellwash 4 Mk 2 conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromag...

Page 81: ...h 4 Mk 2 110 120 V AC 60 Hz 10 1 List of accessories Code Product N03283 2 l wash bottle 1514313 2 l waste bottle WW004 1 12 way wash head WW004 11 8 way wash head WW004 48 Wash height adapter 10 2 Li...

Page 82: ...fic or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific This guarantee is subject...

Page 83: ...ist 12 maintence 35 Clogging 42 prevention 34 Color coding of the tubes 18 Complaint order 51 Consumables warranty limitations 57 Contamination specimen 27 Control panel 11 Countdown sequence of row i...

Page 84: ...ion 10 inspecting 14 markings 55 safety symbols and markings 7 unpacking 12 K Keypad 10 11 cleaning 36 L Liquid level sensor 10 installation 19 Liquid trap 10 Liquid trap bottle 10 37 overfilling prec...

Page 85: ...rogram card 10 30 Pump unit 10 installation 18 Q Quick Reference Guide 63 R Reagent bottle 10 37 Reset button 11 27 28 34 Rotary switches 32 Row select buttons 11 Row select indicators 11 countdown se...

Page 86: ...indicator 11 Vacuum Fail indicator 27 28 49 Voltage 15 Volume 33 W Warning signal 30 Warranty 12 16 57 Wash arm assembly 10 Wash bottle 10 installation 21 Wash head height 22 holder 17 installation 18...

Page 87: ...be detected and measured RS 232C EIA approved standard used in serial data transmission covering voltage and control signals The data is transferred serially one digital bit at a time via one path bu...

Page 88: ...the wash head see Fig 5 13 The POWER indicator on the keypad Fig 4 2 illuminates and a countdown sequence will begin on the row indicator LEDs The PRIME indicator flashes until you prime the system Th...

Page 89: ...ore returning any instrument s or item s ensure that they are fully decontaminated Confirm A or B status Name Address Tel Fax Name Serial no A I confirm that the returned items have not been contamina...

Page 90: ...rnet home page Date of purchase Your research area Dr Mr Mrs Ms Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above As Below Comments expectatio...

Page 91: ...7 Appendix D Addresses For the latest information on products and services visit our worldwide web sites on the Internet at http www thermo com Manufactured by Distributed by Thermo Fisher Scientific...

Page 92: ...Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 68...

Page 93: ...Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 69...

Page 94: ...Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 70...

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Page 110: ...Only for old WW4 ONLY FOR OLD WW4 MODELS...

Page 111: ...ONLY FOR OLD WW4 MODELS...

Page 112: ...ONLY FOR OLD WW4 MODELS...

Page 113: ...1 i t c 1 itc t i n ONLY FOR OLD WW4 MODELS...

Page 114: ...n in all WW 4 mk2 units the vacuum fail switch is in the back of the pump unit You can adjust it by inserting a Phillips screw driver into white plastic hole in bottom left corner at the back of the p...

Page 115: ...SECT103 3 PARTS LIST WW004 010 Pressure Gauge Vacuum Flow Gauge Declogging Tool 3mm Allen Key 2 5mm Allen Key 2 0mm Allen Key Spanner Feeler Gauges Programmable Card Range 0 15 p s i Range 0 8 bar...

Page 116: ...4 052 Washer Cover Assembly 004 052 Items List 1 Sheet 004 053 Pump Unit General Assembly 004 053 Items List 2 Sheets 004 054 Pump Unit Chassis Assembly 004 054 Items Lists 2 Sheets 004 066 Control Lo...

Page 117: ...e Fill Aspirate routine is required to be followed by an Aspirate Fill routine this would allow the user to get rid of any high concentration waste prior to washing with a built in soak time Switch se...

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Page 120: ...WELLWASH 4 4Mk2 SERVICE MANUAL Document 004 006 100 X Release 3 0 g Page 1 Spare parts Wellwash 4 4Mk2...

Page 121: ...rts Contents SUBJECT PAGE Cover page 1 Contents 2 Main parts 3 Washer track assembly 4 Control assembly 6 Pump unit assembly 8 Miscellaneous 10 Accessories 11 NOTE All Spare parts that are considered...

Page 122: ...POS ITEM CAT 1 Carriage assembly 004 084L2 2 Membrane panel 004 644 3 Washer track assembly See page 4 004 052L2 4 Control assembly 230V See page 6 006 053AL2 Control assembly 115V See page 6 006 053...

Page 123: ...WELLWASH 4 4Mk2 SERVICE MANUAL Document 004 006 100 X Release 3 0 g Page 4 WASHER TRACK ASSEMBLY 3 2 1 6 7...

Page 124: ...elease 3 0 g Page 5 Spare parts Washer track assembly POS ITEM CAT 1 Track plate assembly 004 055C 2 2 way pinch valve 000717019 3 Manifold assembly 004 060L2 4 Solenoid grounding wire 004 088L1 6 L h...

Page 125: ...WELLWASH 4 4Mk2 SERVICE MANUAL Document 004 006 100 X Release 3 0 g Page 6 CONTROL ASSEMBLY 5 2 6 3 4 9 10 8 1 7 11...

Page 126: ...V P O 1 004 075BL1 3 Fuse frame assembly P O 1 004 072L1 4 Mains filter assembly P O 1 004 073L1 5 Track motor assembly 230V P O 1 006 076AL1 Track motor assembly 115V P O 1 006 076BL1 6 Headlift moto...

Page 127: ...WELLWASH 4 4Mk2 SERVICE MANUAL Document 004 006 100 X Release 3 0 g Page 8 PUMP UNIT ASSEMBLY SILENCER SILENCER FILTER 1 3 6 9 7 4 8 2 5...

Page 128: ...ain switch female 004 086L1 3 Outlet tube assembly 004 511L1 4 Vacuum switch 1204460 5 Vacuum tube assembly 006 069L1 6 Pump assembly 230V 004 067A Pump assembly 115V 004 067B 7 Pump control pcb 15143...

Page 129: ...1210840 Fuse 1 0A 220 240V 1210890 Fuse 2 0A 110 120V 1210920 Fuse 0 5A 110 120V 000716001 Silicone tube 3 18 x 1 98 000788004 Silicone tube 5 0 x 1 5 000788005 Silicone tube 5 5 x 2 5 AM50 019 Pump S...

Page 130: ...12C1 3 Plate Holder WW004 20 Overflow Bottle WW004 21 2 litre Wash or Waste Bottle with plain cap WW004 22 Adaptor Plate for Flexible microplates WW004 27 Remote Pump Extension Kit WW004 28 Spare O r...

Page 131: ...WELLWASH 4 4Mk2 SERVICE MANUAL Document 004 006 100 X Release 3 0 g Page 12 2 Used in instruments manufactured after the serial number 004 078 3 For WW4mk2 4 For instruments made after s n 006 9 4693...

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Page 133: ...E RUBBER 1 1 1 13 1 0007700131 SPRING 1 1 1 114 000820031 CIRCLIP 1 1 1 15 000883001 1 SILICON SEALANT I I 16 1 0008020491 SCREW M3 x 8 PANHEAD POZI 1 2 1 18 0008040761 WASHER M3 WAVE 1 8 1 1 00080302...

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Page 135: ...1 9 APR 1996 t 2 I 20 j000804078 2 1 BILLINGSHURST SUSSEX ENGLAND WITHOUT THE URITTEN CONSENT OF CHECKED THE COMPANY 1994 FOX I I W A S H E R H4 W A V E I 7 l f w 3 2 TITLE DRAUN YII GENERAL ASSEHBLY...

Page 136: ...31 DOT MARKER ADHESIVE BACKED BLUE R rlAmS 6mn 12mm1 1 1 4 DIA I I I I 41 1 000881242 LOCTITE NUTLOC 242E 1 d 12 7 I D 12 7 I D APPROVED S DOWNES 37 38 39 CHECKED J FOX DRAWN D NAPPER t DOT MARKER ADH...

Page 137: ...308 BOTTLE C A P MOD 1 1 13 1 000543901 1 CABLE T I E BASE 1 1 I 114 1 000839003 BOTTLE 1 2 1 DENLEY IWl LTD COPIES IN I 15 16 17 1 8 19 1 TITLE 1 1004 1 XILIST FOA DAE k 1 000839009 000921006 000800...

Page 138: ...KED RED 1 4 D I A 000891004 DOT MARKER ADHESIVE BACKED YELL 1 4 D I A 138 000891006 1 DOT MARKER ADHESIVE BACKED BLACK I 1 1 4 D I A TITLE 41 10008812421 LOCTITE NUTLOC 242E I A OTY RaWKS BILLINGSWURS...

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Page 140: ...TCH 1 15 000727005 PIGGY BACK CRIMP CONNECTOR 2 16 000727002 SHROUDED RECEPTICAL 3116 6 17 18 000743017 FRICTION BUSH 2 19 000780007 M4 EARTH TAG 2 SIPROOF 20 000891001 EARTH SYMBOL 1 21 SLEEVING HZ0...

Page 141: ...3010 NUT M4 FULL HEX 6 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 OENLEY INSTRUMENTS LTD COPIES IN WHOLE OR PART MUST NOT BE MADE WITHOUT THE WRITTEN CONSENT i i 1 1 1 12 4 1 96 6468...

Page 142: ...YMBOL SLEEVING HZ0 x 20 WlRE 20 AWG 10130 GREY WlRE 18 AWG 16 30 GREYNLLW WIRE 20 AWG 10130 BLUE WlRE 20 AWG 10130 BROWN WIRE 20 AWG 10130 RED CABLE TIE BASE CABLE TIE LABEL TRANSIT SCREW BARBED CONNE...

Page 143: ...fflDENLEY Billingshunt Sussex RH14 9EY England 51 000803008 NUT M3 FULL HEX 4 52 53 54 55 000803010 NUT M4 FULL HEX 6 SSiS ASSEMBLY...

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Page 145: ...R NEWORK 5X1K CERAMIC WMI RESISTOR 4 7R 4W VOLTAGE REGUMTOR uA 7805UC VOLTAGE REG L78SlZCV 12V 2A HIGH SPEED SUPPRESSOR 6 8V QUARTZ CRYSTAL HC18U 6MHZ TRIAC MAC 97 6 RESISTOR NETWORK 8x47K RECTIFIER K...

Page 146: ...BLOCK SMKD 5312 5 08 3 TB1 2 4 63 000791009 3 WAY TERM BLOCK SMKD 313 5 08 1 TB3 64 000794004 CARD CONNECTOR 1 EC1 65 000730001 HEATSINK 1 FOR REG1 66 000794002 RIGHT ANGLED HEADER 10 WAY 10136 PL7 6...

Page 147: ...2 pF 100VPLATE CERAMIC 2 C1 2 24 000702009 0 1 uF 25V DISC CERAMIC 11 C7 TO C15 C20 C22 25 000702005 0 01 uF 400V POLYESTER 3 C17 TO C19 26 000702172 820 uF lOOv SINGLE LAYER CERAMIC 5 C23 TO C27 27 2...

Page 148: ...R 2 WAY TERM BLOCK SMKD S312 5 08 3 WAY TERM BLOCK SMKD S313 5 08 CARD CONNECTOR HEATSINK RIGHT ANGLED HEADER 10 WAY IC SOCKET 40 WAY LED SPACER SPACER 2Omm TRANSISTOR PAD FISH BEADS RED HELSYN SLEEVE...

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