8.
(Optional for fluorescent images) Touch the dye drop-down menu to select a
particular dye or the membrane view.
9.
(Optional) Touch More options for image adjustments or to generate a report.
Image adjustments
Functions
Adjust lanes
Add, remove, or edit lanes.
Adjust bands
Add, remove, or edit bands.
Marker
Identify a molecular weight lane for each analysis frame. A
known standard can be used to approximate the molecular
weight of unknown bands.
Generate report
Prepare a pdf report displaying the images and analysis
table associated with the image file. This file can be
exported and/or printed.
After each image adjustment:
a.
Touch Apply to accept the changes.
b.
(Optional) Touch Next to display the analysis table. At the top of the table,
touch
to change the table output by:
Output
Units
Description
Volume
Intensity
Sum of pixel intensities contained in an
identified band.
Area
Pixels
Total number of pixels contained in an
identified band.
Density
Intensity/Area Average intensity for all pixels contained in
an identified band (Volume divided by Area).
Local
Background
Corrected
Volume
Intensity
Volume minus the local background
intensity (2-pixel area surrounding the
identified band region).
Local
Background
Corrected
Density
Intensity/Area Local Background Corrected Volume divided
by the Area.
Migration
Distance
R
f
Distance migrated divided by the gel length.
Molecular
Weight
kDa
Molecular weight estimated for an unknown
sample based on the sample's relative
mobility in the gel versus the molecular
weight and relative mobility of known
standards.
Export and analyze images
Analyze images
iBright
™
Imaging Systems User Guide
25
