Protein Gels
• Use this mode with the provided white screen to image protein gels stained with
colorimetric stains such as Coomassie and silver stains.
• Use this mode without the white screen to image proteins transferred to
membranes that have been stained with colorimetric stains such as Ponceau S and
Pierce
™
Reversible Membrane Stain.
• Use this mode without the white screen to image protein gels stained with
SYPRO
™
dyes.
IMPORTANT!
For visual stained gels (e.g., Coomassie or silver-stained gels), place
white screen under the sample.
1.
Touch Smart exposure. Alternatively, to set a manual exposure time, touch the
center of the dial.
The imager acquires a series of short exposures and then renders a preview
image and a recommended exposure time. This is not a real image.
2.
To adjust or set a manual exposure time, select a method:
• Touch or within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
3.
Touch Enter.
The image preview will update in real-time.
4.
Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
5.
If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options
4
Image adjust to optimize image or touch Trash to
remove image, then return to step 2 to adjust exposure conditions.
Image using
Protein Gel
Operate the iBright
™
imager
Protein Gels
16
iBright
™
Imaging Systems User Guide
