ThermoFisher Scientific Invitrogen iBright CL1000 User Manual Download Page 1

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For Research Use Only. Not for use in diagnostic procedures.

iBright

™

Imaging Systems

USER GUIDE

For use with the iBright

™

CL1000 Imaging System and the iBright

™

FL1000 Imaging System

Catalog Numbers

A32749 (CL1000) and A32752 (FL1000)

Publication Number

MAN0015702

Revision

A.0

Summary of Contents for Invitrogen iBright CL1000

Page 1: ...n diagnostic procedures iBright Imaging Systems USER GUIDE For use with the iBright CL1000 Imaging System and the iBright FL1000 Imaging System Catalog Numbers A32749 CL1000 and A32752 FL1000 Publicat...

Page 2: ...THIS DOCUMENT INCLUDING YOUR USE OF IT Revision history Pub No MAN0015702 Revision Date Description A 0 May 2017 New document Describes installation operation and maintenance of the iBright Imaging S...

Page 3: ...the iBright imager 11 Choose imaging mode and start image capture 11 Chemi Blots 12 Capture an image of the sample 12 Fluorescent Blots FL1000 model only 13 Image using Fluorescent Blots FL1000 model...

Page 4: ...ment 30 Prepare for shipping 30 APPENDIX A Instrument specifications 31 Instrument dimensions and specifications 31 Electrical requirements 31 Environmental requirements 32 Network requirements 33 Ins...

Page 5: ...gnetic compatibility EMC standards 41 Safety compliance 41 EMC 41 Environmental design 42 Chemical safety 43 Biological hazard safety 44 Documentation and support 45 Related documentation 45 Customer...

Page 6: ...id stains and visible protein gels The iBright FL1000 Imaging System adds the capability of imaging fluorescent Western blots using multiple dyes The iBright imagers use a simple design and intuitive...

Page 7: ...ample placement Contents Table 1 Included imager system parts Component Quantity Power cord region specific 1 Quick reference card 1 White transilluminator screen 1 Reference target 1 Sample stage tur...

Page 8: ...rofile to your Thermo Fisher Cloud account on page 8 Link a new profile to your Thermo Fisher Cloud account From your new My Profile screen 1 Touch Cloud 2 Enter your Thermo Fisher Cloud account usern...

Page 9: ...le Note For those not logged on as a user guest acquired images are saved to a guest gallery Images cannot be transferred between user and guest galleries 3 Enter a Screen name 4 Enter and confirm a f...

Page 10: ...transferred between user and guest galleries 2 From the Welcome screen choose the appropriate Mode from the drop down menu touch Open Drawer and place sample in the center of the turntable glass 3 Tou...

Page 11: ...n drawer 3 Place sample in the center of the viewing area 4 Touch to close drawer Instrument automatically aligns zooms focuses and acquires a live view sample image To image in Chemi Blots mode proce...

Page 12: ...then renders a preview image and recommends an exposure time This is not a real image 2 Optional Adjust the exposure time using the following methods Touch or Finger swipe in the segmented dial Touch...

Page 13: ...time or touch Custom to manually input a time Note To adjust or set the exposure time for a specific dye channel see Edit dye channel exposure time on page 14 5 Touch Enter The image preview will upda...

Page 14: ...To adjust or set a manual exposure time select a method Touch or within dial Finger swipe in the segmented dial Touch the dial center box to select a preset exposure time or touch Custom to input a u...

Page 15: ...ch or within dial Finger swipe in the segmented dial Touch the dial center box to select a preset exposure time or touch Custom to manually input a time 3 Touch Enter The image preview will update in...

Page 16: ...series of short exposures and then renders a preview image and a recommended exposure time This is not a real image 2 To adjust or set a manual exposure time select a method Touch or within dial Fing...

Page 17: ...period Signal accumulation Series of exposures where user defines the first capture time interval the last capture cumulative capture time and the total number of captures Signal accumulation Note Im...

Page 18: ...Manual exposure Manually select an exposure time for sample Smart exposure Predicts optimal exposure for minimizing pixel saturation and maximizing dynamic range for a specific sample Renders a previe...

Page 19: ...splayed as different false colors overlaid on a black background Individual channels can be toggled on and off in the composite image by touching Edit channels and on the left of the appropriate dye 1...

Page 20: ...the Welcome screen or mode drop down menu 1 Touch Gallery or 2 Toggle between Thumbnail view and List view to see stored images as thumbnails or a table Click the column headers to sort the images 3 T...

Page 21: ...information may be required to map a network drive Drive location Enter the IP address server name and file pathway use forward slashes Example 192 168 1 100 shared R D Data John_Smith 2016 Additional...

Page 22: ...rom the Export images screen 1 Touch Choose File Type 2 Touch Publication or Analysis to select the intended use 3 Touch a file type File type Description Publication Exports 24 bit color adjusted ima...

Page 23: ...isting analysis frames 5 Touch Apply to accept any changes 6 Optional for fluorescent images Touch the dye drop down menu to select a particular dye or the membrane view 7 Optional Touch More options...

Page 24: ...sus the molecular weight and relative mobility of known standards On the Welcome screen or capture screen Mode drop down menu 1 Touch Gallery or Select one or multiple images 2 Touch Actions 3 If more...

Page 25: ...top of the table touch to change the table output by Output Units Description Volume Intensity Sum of pixel intensities contained in an identified band Area Pixels Total number of pixels contained in...

Page 26: ...Name Customize the instrument name Language Select a display language subject to availability Reset User Tips Reset user tips for the logged in guest Reset Factory Defaults Resets instrument to facto...

Page 27: ...turntable for shipping Note Instrument can only be powered off once turntable is removed Once powered off then powered on you must go through the complete new instrument set up again Manage accounts a...

Page 28: ...r is not blocked The drawer will open as part of the testing Clean the instrument Repeated instrument use can cause spots and smudges on the sample stage which can then decrease image quality Clean th...

Page 29: ...older and inspect it for damage Carbon typically coats the inside of failed fuses 4 Replace each failed fuse with a 1 6A Time Lag T 250VAC 5 20 mm Fuse Note The voltage and amperage ratings are on the...

Page 30: ...y Ensure that the surface on which you place the instrument can support at least 50 kg 110 lbs Ensure that the path to transport the instrument is clear of obstructions At least two people are needed...

Page 31: ...low adequate space between the wall and the equipment so the power cord can be disconnected in case of emergency Electric receptacle with grounding capability Maximum power dissipation 960 W not inclu...

Page 32: ...al output based on the actual current draw of the instrument is expected to be approximately 960 W 3275 Btu h Vibration Ensure that the instrument is not adjacent to strong vibration sources such as a...

Page 33: ...is to install the instrument If the instrument will be connected to a LAN an active tested network jack must be in place before the scheduled installation date A representative from your information...

Page 34: ...computer connections iBright Imager front view 1 2 3 1 Touchscreen 2 USB port 3 Sample stage turntable Appendix A Instrument specifications Instrument and computer connections A 34 iBright Imaging Sys...

Page 35: ...Ethernet port RJ45 port for 10 100 Mbps ethernet communication with the instrument 3 Fuse cover 4 Power switch 5 Power port 100 240 VAC Appendix A Instrument specifications Instrument and computer con...

Page 36: ...ent Symbols may be found on the instrument to warn against potential hazards or convey important safety information In this document the hazard symbol is used along with one of the following user atte...

Page 37: ...ri s lectif Se conformer aux ordonnances locales sur les d chets municipaux pour les dispositions d limination et communiquer avec le service la client le pour des renseignements sur les options d lim...

Page 38: ...f you remove the protective instrument panels or disable interlock devices you may be exposed to serious hazards including but not limited to severe electrical shock laser exposure crushing or chemica...

Page 39: ...y that the fuses are properly installed and the fuse voltage matches the supply voltage Replace fuses only with the type and rating specified for the unit Improper fuses can damage the instrument wiri...

Page 40: ...ty or sending the instrument for repair maintenance trade in disposal or termination of a loan decontamination forms may be requested from customer service Before using any cleaning or decontamination...

Page 41: ...nt for measurement control and laboratory use Part 2 081 Particular requirements for automatic and semi automatic laboratory equipment for analysis and other purposes IEC 62471 1 2007 Photobiological...

Page 42: ...se the user will be required to correct the interference at his own expense Reference Description EU Directive 2012 19 EU European Union WEEE Directive Waste electrical and electronic equipment EU Dir...

Page 43: ...ersonal protective equipment when handling chemicals for example safety glasses gloves or protective clothing Minimize the inhalation of chemicals Do not leave chemical containers open Use only with a...

Page 44: ...nd company institution requirements before working with potentially biohazardous materials Follow all applicable local state provincial and or national regulations The following references provide gen...

Page 45: ...ems touchscreen and provides procedures for configuring the instrument and performing imaging Customer and technical support Visit thermofisher com support for the latest in services and support inclu...

Page 46: ...hnologies General Terms and Conditions of Sale found on Life Technologies website at www thermofisher com us en home global terms and conditions html If you have any questions please contact Life Tech...

Page 47: ......

Page 48: ...thermofisher com support thermofisher com askaquestion thermofisher com 12 May 2017...

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