26 | Operation
Controlled Rate Freezer
Preset_5:
Commonly used for skin packets, resulting in a
1°C rate from nucleation to -35°C and a 10°C per minute
cooling rate to a -90°C end temperature.
Preset_6:
Commonly used for hemopoietic stem cells that
are 65-100ml in size, resulting in a 0.5°C rate from nucleation
to -20°C and a 1°C per minute cooling rate to a -80°C end
temperature.
Add Profile
If you want to add a profile with custom steps, press on Add
Profile on the Profile Screen (
). Up to 14 Custom
Profiles may be added with different names for each profile.
When you add a profile they will be listed below the 6 preset
profiles
Note:
Only Admin users can Add Profiles.
When you press on the Add Profile button you will be
navigated to the following screen:
Figure 62. Add Profile Screen
You may create a profile by assigning the profile name and
steps as needed. There is a maximum of 20 steps per profile.
The Action field of each step must be set to one of the
following commands available in the drop-down menu:
Wait:
WAIT AT X.X°C SET TEMPERATURE.
Figure 63. Wait Command
Wait maintains the specified chamber temperature indefinitely
until the user selects the Advance button on the Active Run
screen. The temperature can be set over the -180°C to
+50°C range, with a setting resolution of 0.1°C.
Wait For:
WAIT AT CHAMBER=X.X°C UNTIL SAMPLE=Y.Y°C.
•
X.X is Chamber set temperature and Y.Y is the Sample
trip temperature.
•
The default Sample trip temperature is 1°C above
Chamber temperature.
Figure 64. Wait For Command.
Wait For maintains the specified Chamber temperature and
moves to next step in profile when Sample temperature cools
Step 3
25.0°C/m C
to -50°C
Step 4
10.0°C/m C
to -14.0°C
Step 5
1.0°C/m C
to -45°C
Step 6
10.0°C/m C
to -90°C
Step 7
End
Step 1
Wait
at 4.0°C
Step 2
1.0°C/m S
to -5.0°C
Step 3
20.0°C/m C
to -40°C
Step 4
10.0°C/m C
to -10.0°C
Step 5
1.0°C/m C
to -35°C
Step 6
10.0°C/m C
to -90°C
Step 7
End
Step 1
Wait
at 4.0°C
Step 2
1.0°C/m S
to -4.0°C
Step 3
20.0°C/m C
to -45°C
Step 4
10.0°C/m C
to -10.0°C
Step 5
0.5°C/m C
to -20°C
Step 6
1.0°C/m S
to -80°C
Step 7
End