Countess
™
II Automated Cell Counter User Guide
3
Contents
About this guide ............................................................................................................ 5
1.
Product information ................................................................................................ 7
Product description ......................................................................................................................................... 7
Instrument exterior components ................................................................................................................... 8
2.
Getting started ........................................................................................................ 9
Installation ........................................................................................................................................................ 9
Load profile .................................................................................................................................................... 11
3.
Cell count and cell viability assays ........................................................................ 15
Prepare sample .............................................................................................................................................. 15
Count cells ...................................................................................................................................................... 16
4.
Results .................................................................................................................. 18
View results .................................................................................................................................................... 18
Identify the cells counted ............................................................................................................................. 19
Graph count results ....................................................................................................................................... 20
Gate count results .......................................................................................................................................... 21
Save as new protocol ..................................................................................................................................... 22
5.
Dilution calculator ................................................................................................. 24
Calculate dilution .......................................................................................................................................... 24
6.
Save results .......................................................................................................... 27
Save count results .......................................................................................................................................... 27
Report .............................................................................................................................................................. 30
7.
Instrument settings ............................................................................................... 31
Overview ........................................................................................................................................................ 31
Software update ............................................................................................................................................. 32
Date/Time ...................................................................................................................................................... 33
8.
Maintenance .......................................................................................................... 35
Instrument care .............................................................................................................................................. 35
Clean the Countess
™
II Automated Cell Counter ..................................................................................... 36
Set nominal focus .......................................................................................................................................... 37
Appendix A: Troubleshooting...................................................................................... 39
Appendix B: Product specifications ............................................................................ 41
Technical specifications ................................................................................................................................ 41
Appendix C: Ordering information .............................................................................. 42
Countess
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II Automated Cell Counter ....................................................................................................... 42
Accessory products ....................................................................................................................................... 42