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Countess
™
II Automated Cell Counter User Guide
21
Gate count results
Adjust screen
The Adjust screen contains the controls for gating count results based on size,
brightness, and circularity. You can adjust the count parameters before or after
performing a count.
Gate count results
1.
On the Results screen, press
More
to open the Advanced screen.
2.
Optional
: Press the
Live
and/or the
Dead
button to identify the cells in the
selected population (see page 19).
3.
On the Advanced screen, press
Adjust
to open the Adjust screen.
4.
Optional
: Press the
Graph
button to view the distribution of cells (live
and/or dead) based on size as you gate the count results (see page 20).
5.
Select the channel
(Live
or
Dead
) you wish to gate.
6.
Using the
size
,
brightness
, and
circularity
sliders
, adjust the count parameters.
Note:
For a description of the count parameters and count parameter controls
(i.e., parameter sliders), see page 12.
7.
When finished, press
Done
to save the changes to count parameters and return
to the Advanced screen.
Press
Cancel
to return to the Results screen without saving the changes.
8.
On the Advanced screen, press
Count
to recalculate your results with the new
count parameters.
9.
To save the changes to size, brightness, or circularity parameters to the current
profile or to create a profile with the new count parameters, see page 22.
10.
To permanently save your results to an external computer using a USB flash
drive, see page 27.