12
Countess
™
II Automated Cell Counter User Guide
Edit profile screen
The Auto Lighting function and count parameters are set in the Edit profile screen.
Auto Lighting
Auto Lighting function automatically illuminates the sample in the brightfield
channel for increased sample-to-sample consistency and decreased user-to-user
variability. By default, the Auto Lighting function is turned on; to toggle it on and
off, use the
Auto Lighting
checkbox (see page 13).
Count parameters
Count parameters are set using the
parameter sliders
, which correspond to a single
channel that is selected via the
channel selection
radio buttons.
•
Size:
As you move the slider up, the algorithm includes larger objects in the
count. As you move the slider down, only the smaller objects are counted.
= larger objects
= smaller objects
•
Brightness:
As you move the slider up, the algorithm includes the brighter
objects in the count. As you move the slider down, only the dimmest of objects
are counted.
= brighter objects
= dimmer objects
•
Circularity:
As you move the slider up, the algorithm includes more objects
with shapes other than circular in the count. As you move the slider down,
only the objects that are perfect circles are counted.
= less circular
= more circular
•
Size
and
brightness
sliders are range sliders.
To adjust the upper and lower boundaries without changing the data range,
drag the slider by its middle section (i.e., the slider bar).
To adjust only the upper or the lower boundary, move the upper or the lower
handle in the desired direction. This will also change the range of values
within which the cells are counted.
•
The
circularity
slider only sets a single threshold value; cells that fall below the
set value are counted, and cells that are beyond this range are excluded.
To adjust the threshold for circularity, drag the slider in the desired direction.
