Thermo Scientific VARIOKLAV 25T Operating Manual Download Page 56

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12 Troubleshooting

03/2007

DA0621_a/

Message in the
cycle display

Meaning Remedy

Error 17
Overheating

Heating system does not turn off.

Heating system relay defective or
program control error.

Contact after-sales
service.

Error 20
Microheating

Microheating system (heatable
temperature sensor) cannot regulate
water feed to jacket.

Microheating system defective.

Contact after-sales
service.

Error 22
No heater

Heating coils are defective, or
the heating system control is
defective.

Contact after-sales
service.

Error 23
Partial heater

One heating coil is defective.

Contact after-sales
service.

Err1

in the Chamber
field:

in the Media field:

Sensor broken

Chamber temperature sensor is
affected.

Flexible temperature sensor
(reference temperature sensor) is
affected

Have technician
replace sensor or
contact after-sales
service.

Err2

in the Chamber
field:

in the Media field:

Sensor shorted

Chamber temperature sensor

Flexible temperature sensor

12)2)2)

(reference temperature sensor).

Have technician
replace sensor or
contact after-sales
service.

Err3

in the Chamber
field:
in the Jacket field:
below the Jacket
field:

Pressure sensor defective

Pressure sensor in sterilizing
chamber
Pressure sensor in jacket

12)

Pressure sensor in seal

12)

Contact after-sales
service.

Table 5: Error codes and their meanings

12)

If present

Summary of Contents for VARIOKLAV 25T

Page 1: ...VARIOKLAVTM Steam sterilizer 25T Operating Manual Issue 03 2007 ...

Page 2: ...etails Dissemination and copying of this document utilization and information of its content are not allowed unless specifically authorized Violations commit you to compensation Subject to change without notice No liability assumed for literal mistakes Thermo Electron LED GmbH is legal successor of H P Labortechnik AG Thermo Electron LED GmbH Robert Bosch Straße 1 D 63505 Langenselbold Germany nat...

Page 3: ... 6 2 Setting the sterilizing temperature 28 6 3 Setting the sterilizing time 29 6 4 Setting the multiple sterilization number 29 6 5 Selecting or deselecting options acoustic signal or integrated printer 30 6 6 Setting the timer 31 7 Starting and running a program 32 7 1 Batch documentation option 34 8 Program end and removal 36 9 Configuring programs 38 10 Initial commissioning 42 10 1 Scope of d...

Page 4: ...ing 51 11 1 After sales service and maintenance 52 11 2 Important notes 52 11 3 Inserting paper rolls into the integrated printer 53 11 4 Changing the ribbon of the integrated printer 54 12 Troubleshooting 55 13 Technical specifications 57 14 Glossary 58 ...

Page 5: ...sumables waste and to destroy infectious or septic materials Ö Sterilizing liquids such as solutions and culture media in open or slightly closed vessels including cooling to a reference temperature below 90 C to allow safe removal Ö Sterilizing food Ö Steaming liquids or dissolving agar agar Ö With Vacuum pump option only Sterilizing and drying porous materials fabric or tubes Ö With Type ST supp...

Page 6: ...re indicated by the word LIFE HAZARD DANGER This sign refers to explosion hazards DANGER This sign refers to dangerous voltages CAUTION This sign indicates danger to equipment and machinery INFORMATION This sign indicates easier working practices Indicates an operating step Ö Indicates alternatives Abbreviations You will find a glossary of technical terms and abbreviations in Chapter 14 ...

Page 7: ...rnings are indicated by the following signs on a grey background DANGER This sign refers to hazardous situations Hazards to human life are indicated by the word LIFE HAZARD DANGER This sign refers to explosion hazards DANGER This sign refers to dangerous voltages CAUTION This sign indicates danger to equipment and machinery INFORMATION This sign indicates easier working practices On the following ...

Page 8: ...ning a sterilization cycle Press the green START STOP key to terminate any ongoing programs if necessary DANGER If a program is interrupted the load must be re sterilized for safety reasons DANGER Only run the sterilizer for correct use DANGER Only sterilize waste in open vessels e g metal boat with closed bottom DANGER Do not open the sterilizing chamber 8 until removal is signalled Watch for hot...

Page 9: ...e emitted from the sterilizer to the on site disposal system Remedy Type FA exhaust air filtration expansion option CAUTION Take care to ensure that the door seal 6 is free from dirt not sticking to the door 2 and not damaged The door seal 6 must sit snugly in the groove CAUTION Before sterilization check to see whether there is enough feed water in the feed water tank 4 Only use deionized or dist...

Page 10: ...any anomalies and or problems occur while the sterilizer is ready for use or while running a sterilization cycle Press the green START STOP key to terminate any ongoing programs if necessary INFORMATION To deactivate the Preheating program press the Preheating key 22 Examples of possible anomalies or problems Steam escapes from behind the cover or from the disposal area Water dropping from the bot...

Page 11: ...rilization Even when all technical prerequisites for safe sterilization exist sterility of the load cannot be guaranteed There is always a residual risk depending in part on the care with which the user and operator perform their respective tasks Omission of the checks for routine maintenance and care enumerated in the Maintenance Manual weekly monthly semi annually and annually Feed water impurit...

Page 12: ...2 Short operating manual 03 2007 12 DA0621_a 2 Short operating manual INFORMATION Attach the short operating manual close to the sterilizer ...

Page 13: ...3 Equipment description 03 2007 13 DA0621_a 3 Equipment description 3 1 Components Figure 1 VARIOKLAV 25 T 1 2 3 4 5 6 7 8 9 10 11 12 ...

Page 14: ...ightly locked to the housing anchors 7 3 Case The case contains the control elements and fittings Zyklus Betrieb Tür Start Stop Konfig P1 P2 P3 Zeit Kammer Mantel Medium Kammer Start Vakuum Aufheizen Sterilisieren Abkühlen Trocknen Zyklus Ende C C kPa kPa min Cycle Operation Door Start Stop Config P1 P2 P3 Time Chamber Jacket Liquid Chamber Start Vacuum Heating Sterilization Recooling Drying End o...

Page 15: ... the chamber 12 Insert tray For placing items in the sterilizing chamber 13 Current values display Indicates the current conditions prevailing in the sterilizing chamber Chamber temperature in C additionally if Media temperature control option is installed Media temperature in C Chamber pressure in kPa additionally if Drying option is installed Jacket pressure in kPa additionally if the Multiple s...

Page 16: ...oduced directly into the sterilizing chamber 8 A rigidly mounted temperature sensor is measuring the chamber temperature The following components are stored in the case 3 the sterilizing chamber 8 the safety valve the feed water tank 4 the control panel 5 The control panel 5 is used to select sterilization parameters and to start the sterilizing program Sterilizing chamber 8 pressure and temperatu...

Page 17: ...ing of liquids culture media and solutions at 98 C The functional description will start by presenting the basic functions of the VARIOKLAV Type 25 T benchtop sterilizers In addition you will find here a description of the expansion options The VARIOKLAV Type 25 T benchtop sterilizer is microprocessor controlled This means that sterilization can proceed without supervision You must set the desired...

Page 18: ...immediately after the sterilized items have been removed and the feed water tank has been filled up if required with deionized water 4 1 Options Ö Media temperature control With this option the sterilizer is additionally equipped with a flexible temperature sensor The flexible temperature sensor measures the media temperature in a reference flask In all Liquids programs the sterilizing timer is no...

Page 19: ...ogram selected and executed must then be terminated manually The feed water tank 4 level must be checked at regular intervals Ö Multiple sterilization The benchtop sterilizer can be reprogrammed for multiple sterilization for testing This means that the complete sterilization cycle is executed several times in succession The number of cycles can be selected This requires the presence of certain ex...

Page 20: ...ference temperature is not shown The sterilizer may be equipped additionally with a flexible temperature sensor media temperature control expansion option This flexible temperature sensor has a pressure sealed entry point through the back wall of the sterilizing chamber In all Liquids programs the flexible temperature sensor measures media temperature as the reference temperature This temperature ...

Page 21: ... By pressing the START STOP key 19 the sterilizer can be operated as usual Push the green START STOP key 19 to switch on the sterilizer The settings of the last sterilizing program selected are shown on the cycle display 16 The current values display shows The chamber temperature The media temperature if the Media temperature control expansion option is present The pressure in the sterilizing cham...

Page 22: ...cessary fill the feed water tank 4 with deionized or distilled water Deaeration nozzle Max Min Figure 4 Feed water tank filling level 4 Feed water supply Consumption per batch Approximately 7 litres Approximately 0 2 1 5 litres Table 1 Feed water If the feed water tank 4 runs dry during program execution the program is automatically terminated and an error message is shown on the cycle display 16 ...

Page 23: ... tips these must be permeable to steam Boxes must not be stacked on top of each other Pipette tips must not be sterilized in closed boxes Pipette tips will be moist following sterilization Select the Instruments program Ö Destruction Waste INFORMATION Only sterilize waste in open vessels e g metal boat with closed bottom Place the waste in open vessels or bags or a metal tray with a solid bottom P...

Page 24: ...ding the appropriate amount of liquid Liquid loss can be reduced by covering vessels with aluminium foil or a cellulose stopper If you use laboratory flasks made of boron silicate glass such as DURAN you may loosely fit the screw cap or screw it on by no more than one turn For sterilizing liquids a reference temperature is determined With media temperature control expansion option Measure the medi...

Page 25: ...erature as the reference temperature is measured using the flexible temperature sensor The following points should be observed in connection with the reference flask It must be at least as large as the vessels for the sterilizing articles It must be filled at least as far as the vessels for the sterilizing articles It must be placed in the back of the tray area The reference flask is filled with t...

Page 26: ...ling system option only Liquids RM 121 C 20 min Water jacket cooling program end at 90 C reference temperature With media temperature control expansion option only Liquids Liquids RM Liquids RO Liquids RG 121 C 20 min Sterilizing time actuated via media temperature Depending on program see above program end at 90 C4 media temperature With Type FA exhaust air filtration only All programs Deaeration...

Page 27: ...hat the liquid loss remains low In the Liquids program the sterilization parameters except the vacuum and the options may yet be changed How you choose a program is described in Chapters 6 1 to 6 6 There you also will find information which values should be chosen for each kind of sterilizing articles How to set the timer so that a sterilization cycle may automatically start at a later point in ti...

Page 28: ...trol is present media temperature of 90 C 6 2 Setting the sterilizing temperature The selected set temperature in C is shown on the cycle display 16 The total available temperature range will depend on the operating data of your sterilizer The following range is valid for Germany6 Type 25T 98 C to 135 C We recommend the following set temperatures for sterilization and disinfection 98 C for disinfe...

Page 29: ...rilizing temperature appears in the cycle display Figure 8 Cycle Steril Time 20min Figure 8 Sterilizing time display Increase or decrease the sterilizing time using the key 14 or the key 15 The sterilizing time will increase or decrease in one minute increments with each press of the key 14 or the key 15 When decreasing sterilizing time the display will change from the minimum selectable to the ma...

Page 30: ...he indicator in the Acoustic signal key 21 is lit You may turn off the acoustic signal before starting the program Press the Acoustic signal key 21 The signal is turned off the indicator in the Acoustic signal key 21 is off Ö Batch documentation If the benchtop sterilizer is equipped with an integrated printer this printer can operate in parallel with any program First you should check the date an...

Page 31: ...month year or hours minutes seconds press the program key below and change the value using the key 14 or the key 15 or P1 P1 P2 P2 P3 or or day hours month minutes year or or or Cycle 30 01 07 Timer Date Cycle 12h 20min Timer Time Figure 10 Setting the timer with corresponding display Example Setting the hours Use the key 14 or the key 15 to change to the Timer display Press and hold the P1 progra...

Page 32: ...e pressure subsided The sterilizing chamber 8 is automatically heated and deaerated The various phases of the program are shown in the sterilization phase display 24 during execution Assuming normal loading of the sterilizing chamber 8 with liquid sterilizing articles you will need a heating time of approximately 25 to 35 minutes The sterilizing chamber 8 will then have reached a temperature of 12...

Page 33: ...rilization number In the Liquids programs the thermolock will unlock the door 2 as soon as the reference temperature drops below 90 C By pressing the START STOP key 19 you can interrupt the program cycle at any time The sterilizer always returns to a safe state before unlocking the door If the program is followed by a drying phase the current values display 13 shows the drying time and the symbol ...

Page 34: ...1 12 95 2 96 9 100 21 22 105 5 120 9 150 STERILIZING 21 31 121 2 122 3 320 21 32 121 2 122 9 320 21 33 121 2 122 9 320 21 34 121 2 122 3 320 21 35 121 2 122 9 320 21 36 121 1 122 9 320 21 37 121 2 122 3 320 21 38 121 2 122 9 320 21 39 121 3 122 9 320 21 40 121 2 122 3 320 21 41 121 2 122 9 320 21 42 121 2 122 9 320 21 43 121 2 122 3 320 21 44 121 2 122 9 320 21 45 121 2 122 9 320 21 46 121 0 122 9...

Page 35: ... software lets you log measurements on a PC SEGO Soft displays the readings of all temperature sensors and the chamber pressure sensor in graphic mode Measurements may be stored and archived Usable PC interfaces serial interface RS232 network Ethernet Figure 13 SEGO Soft screen ...

Page 36: ... a buzzer will sound and the green START STOP key 19 will flash This indicates that all phases of the sterilization cycle have been completed The sterilization phase display 24 shows the indicator lights for all completed phases DANGER You must wear the following protective items when opening the sterilizing chamber 8 and removing the sterilized articles y Apron y Eye protectors y Heat insulated g...

Page 37: ...RMATION Without the use of additional drying equipment filters fabric or cotton wool will not dry properly after sterilization INFORMATION After sterilization while using a flexible temperature sensor Remove the flexible temperature sensor from the reference flask of leave the reference flask in the sterilizing chamber 8 Remove sterilized items The sterilizer is ready for a new cycle immediately a...

Page 38: ...terilizer is in operation Carefully store the key in a safe place INFORMATION The program key P3 is solely designed for activating the Liquids sterilization program Authorized operators can thus define individually configured programs for the program keys P1 or P2 Ö Starting configuration Insert the key into the key operated switch 17 Ö Reprogramming a program key Select the one of the both progra...

Page 39: ...e key 15 Ö Setting the removal pressure INFORMATION The removal pressure must be assimilated to the ambient pressure of the installation site If the selected removal pressure is too high steam may escape out of the lid at program end If the selected removal pressure is too low the necessary criteria for ending the sterilizing program can not be achieved Using the key 14 or the key 15 select a valu...

Page 40: ...r date as currently set Using the key 14 and the key 15 you can switch between date and time displays To change the settings for day month year or hours minutes seconds press the program key below and change the value using the key 14 or the key 15 Example Changing over to daylight saving time summer time moving the clock ahead one hour Change to the Time display using the key 14 or the key 15 Pre...

Page 41: ...struments DR Destruction Waste DR programs V Vacuum and drying with vacuum F Type FA exhaust air filtration Z Multiple sterilization C Batch documentation K Climatic chamber E External steam Ö Ending configuration Turn the key operated switch 17 to the operation position Remove the key from the key operated switch ...

Page 42: ...imilated to the ambient pressure of the installation site See Chapter 9 If the selected removal pressure is too high steam may escape out of the lid at program end If the selected removal pressure is too low the necessary criteria for program end can not be achieved INFORMATION Only use the condensate collector if you just utilize the sterilizer occasionally When the collector overflows water dama...

Page 43: ...ents are supplied with the delivery as invoiced Declaration of Conformity for the unit pursuant to the Pressure Equipment Directive DGRL 97 23 EC Certificate for the thermal safety system to prevent retarded boiling Thermolock according to TRB 402 The unit comes with the following documentation Operating Manual with commissioning maintenance and care instructions Short Operating Manual Checklist f...

Page 44: ...rsuant to the Pressure Equipment Directive 97 23 EC Article 4 1 1 110 are met 10 3 2 Operating the device according to the German Operating Safety Regulation When operating the VARIOKLAV Type 25 T steam sterilizer please refer to the national regulations In Germany operators please refer to the German Operating Safety Regulation 10 4 Installation The VARIOKLAV Type 25 T bench top sterilizer can be...

Page 45: ...em CAUTION During the heating phase contaminated exhaust air or waste water may be emitted from the sterilizer to the on site disposal system Remedy Expansion option Type FA exhaust air filtration Exhaust air spent steam condensate approximately 0 5 to 1 litres per batch and waste water are generated during the whole sterilization process The outlets situated at the back of the sterilizer must be ...

Page 46: ... and cooling exhaust air In this case an on site disposal line is not necessary Even in safety laboratories the condensate collector can be used to good advantage as the on site disposal system will remain uncontaminated Plastic container made of temperature resistant PEHD rectangular with two handles Dimensions L x W x H 275 x 235 x 405 mm 20 litres volume Large cleaning hole Ø 140 mm with screw ...

Page 47: ... to on site cooling circuit is possible Cooling water approximately 5 to 10 litres per batch Please observe our Installation and Maintenance Manual Automatic spent steam condenser water cooled They include detailed information regarding the required connections maintenance information and important safety warnings Ö Integrated spent steam condenser with condensate return When on site disposal syst...

Page 48: ...ation Water post feed and Batch documentation expansion options do not need to be commissioned separately They are ready for use when the benchtop sterilizer is delivered 10 5 1 Type RM fast recooling system Required connections Decalcified cooling water or deionized water or very soft tap water of no more than 5 dH German hardness 0 5 bar minimum supply pressure max 6 bar R on site connection max...

Page 49: ...thin the framework of quality assurance during production or in safety laboratories sterilization data may have to be monitored Several fixed and flexible sensors are available for measuring media temperature local chamber temperature s and chamber pressure Measured values are logged using the integrated printer or a PC Integrated printer The integrated printer logs the entire sterilization proces...

Page 50: ...validations It also facilitates batch documentation date sterilization data etc We recommend the SEGOsoft software by Comcotec Messtechnik GmbH for analyzing the sterilization data Users may also control the RS 232 port to run their own programs for process control ...

Page 51: ...ns supply Pull the mains plug or neutralise the fuse INFORMATION When introducing Deodorant Pearls into the sterilizing chamber 8 in order to avoid unpleasant odours Some ingredients of the Deodorant Pearls may rapidly disintegrate the door seal 6 INFORMATION Only original seals made by the manufacturer may be used INFORMATION We highly recommend you to have the sterilizer serviced regularly by ou...

Page 52: ...e g screw caps the sterilizing chamber 8 or the door 2 may exhibit a rust film despite their stainless steel material This film may occur if iron dust is deposited on the door 2 or on the walls or floor of the sterilizing chamber 8 If the sterilizing chamber 8 comes in contact with inorganic acids either when cold or when hot the surface of the pressure vessel may be corroded Even plaques such as ...

Page 53: ...own in Figure 18 Figure 17 Cutting the end of the paper roll Press both the Printer ON OFF key 26 and the Paper feed key 27 concurrently The printer cover 25 opens Remove the printer cover 25 Cut the paper strip off the old paper roll Press the Paper feed key 27 until the remaining paper has been removed from the printer head Remove the old paper roll from the paper roll compartment Insert the new...

Page 54: ...r Press both the Printer ON OFF key 26 and the Paper feed key 27 concurrently The printer cover 25 opens Remove the printer cover 25 Hold the left end of the ribbon cartridge with your left hand Press on the right end of the cartridge This will lift the left end of the cartridge Remove the cartridge Take a new cartridge Insert the left end of the new cartridge in the left corner of the printer hea...

Page 55: ... missing or defective or pressure line missing or interrupted or door seal missing Contact after sales service Error 4 Seal defective Door seal builds but cannot keep pressure Seal leaking or pressure tank leaking or pressure line leaking Contact after sales service Have leaking part replaced Error 6 Compressor Door seal loses pressure during sterilization Compressor defective Contact after sales ...

Page 56: ...defective Contact after sales service Err1 in the Chamber field in the Media field Sensor broken Chamber temperature sensor is affected Flexible temperature sensor reference temperature sensor is affected12 Have technician replace sensor or contact after sales service Err2 in the Chamber field in the Media field Sensor shorted Chamber temperature sensor Flexible temperature sensor 12 2 2 reference...

Page 57: ... contact 16 AT Permissible operating conditions Ambient temperature Relative humidity Barometric pressure 5 C to 40 C 30 to 80 900 hPa to 1 060 hPa Permissible storage conditions empty Ambient temperature Relative humidity Barometric pressure 40 C to 70 C 10 to 95 500 hPa to 1 060 hPa Protective class IP type of protection control I EMC electromagnetic compatibility Integrity test pursuant to DIN ...

Page 58: ...isease causing agents Distilled water Water free of minerals and salts Drying time Time needed for drying E Exhaust air filtration Filtration system for safe sterilization of infectious material with pathogen bioburden Cleanses the exhaust air from the sterilizer during the heating and sterilizing phases Equilibration time Time period until all sterilizing articles have reached the chamber tempera...

Page 59: ...e temperature of liquids and for dissolving agar Steam release Removal of steam from the sterilizing chamber Steam sterilizer Equipment for carrying out degerming under the effect of steam Sterile venting Venting with decontaminated sterile air To sterilize To degerm to eliminate all micro organisms Sterilizer Equipment used for sterilizing Sterilizing articles Liquids or solids destined for deger...

Page 60: ...14 Glossary 03 2007 60 DA0621_a ...

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