118
Thermo Fisher Scientific
User Guide STP420D/STP420ES
appendix F
Preferred Protocols and Best Practices
appendix F
Preferred Protocols and
Best Practices
Note!
The protocols listed here are examples of common procedures. Depending on the
task and type of samples to be treated, it may be necessary to adapt the protocols.
Program step
time (mins)
Pressure/
vacuum (P/V)
temp (0°C) Drain time
(secs)
time to fill and
empty (mins)
overnight
1
Formalin 1
30
P/V
0
20
4
2
Formalin 2
60
P/V
0
40
4
3
70% alcohol
30
P/V
0
20
4
4
80% alcohol
30
P/V
0
20
4
5
95% alcohol
60
P/V
0
20
4
6
100% alcohol
60
P/V
0
20
4
7
100% alcohol
60
P/V
0
40
4
8
Xylene 1
30
P/V
0
20
4
9
Xylene 2
60
P/V
0
20
4
10
Xylene 3
60
P/V
0
40
4
11
Paraffin 1
30
P/V
62
20
4
12
Paraffin 2
30
P/V
62
20
4
13
Paraffin 3
30
P/V
62
20
4
14
Paraffin 4
60
P/V
62
40
4
Total time including time to fill
and empty (hr:min)
11:32
Biopsy
1
Formalin
30
P/V
0
60
4
2
Alc 80%
15
P/V
0
60
4
3
Alc 95%
15
P/V
0
20
4
4
Alc 100%
15
P/V
0
20
4
5
Alc 100%
30
P/V
0
60
4
6
Xylene
15
P/V
0
20
4
7
Xylene
30
P/V
0
60
4
8
Paraffin
15
P/V
62
20
4
9
Paraffin
15
P/V
62
20
4
10
Paraffin
30
P/V
62
20
4
Total time including time to fill
and empty (hr:min)
04:16
F-1 Protocols
