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50152023 is the original instruction manual.

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Summary of Contents for Sorvall ST 8FR

Page 1: ...Thermo Scientific Sorvall ST 8FR Centrifuge Instruction Manual 50152023 e 03 2020 ...

Page 2: ...1 Technical Data 12 Thermo Scientific Sorvall ST 8FR Centrifuge 12 Available Thermo Scientific Rotors 13 2 Directives Standards and Guidelines 14 3 Mains Supply and Refrigerant 15 II Transport and Set Up 16 1 Before Setting Up 16 2 Location 16 3 Transporting 17 4 Mains Connection 19 5 Leveling 20 6 Storage 22 7 Shipping 22 III Control Panel 23 Sorvall ST 8FR 23 IV Operation 24 1 Switching on the C...

Page 3: ... 29 6 Programs 30 Saving a Program 30 Loading a Program 31 Programs Only Mode 31 7 Centrifugation 31 Maximum Loading 31 Before a Run 32 Starting the Centrifugation 32 Imbalance Indicator 33 Stopping the Centrifugation 33 8 Short term Centrifugation 34 9 Removing the Rotor 34 10 Aerosol tight Rotors 35 11 Switch off the Centrifuge 35 V User Menu 36 Navigation within the User Menu 36 VI Maintenance ...

Page 4: ...ion 43 5 Decontamination 44 6 Autoclaving 45 7 Maintenance 45 Preventive Maintenance 45 Service 46 8 Shipping and Disposal 46 VII Troubleshooting 47 1 Mechanical Emergency Door Release 47 2 Error Messages 48 3 When to contact Customer Service 50 Chemical Compatibility Chart 51 Index 64 ...

Page 5: ...ory product used to separate substance mixtures of different densities This centrifuge can become an In vitro diagnostics device Directive 98 79 EC if used together with the hematocrit rotor and his accessories The hematocrit value is determined using the instructions written in the instruction manual of the hematocrit rotor The instructions are based upon the reference method following DIN 58933 ...

Page 6: ...sher Scientific representative Item Quantity Thermo Scientific Sorvall ST 8FR centrifuge 1 Power supply cable 1 Bubble level 1 Wrench 30 mm 1 Instruction manual 2 Signal Words and Colors WARNING Indicates a hazardous situation that if not avoided could result in death or serious injury CAUTION Indicates a hazardous situation that if not avoided could result in minor or moderate injury NOTICE Indic...

Page 7: ...stances within this safety zone Implement measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running Set Up Conditions WARNING Plug the centrifuge only into sockets which have been properly grounded Set up in a well ventilated environment on a horizontally leveled and rigid surface with adequate load bearing capacity The mains plug must b...

Page 8: ...ructions in section Rotor Installation on page 25 Do not use a rotor or accessories that show any signs of corrosion or cracks Contact customer service for further advice or inspections Use only with a rotor that has been properly loaded Never overload the rotor Always balance the samples Use only rotors and accessories for this centrifuge that have been approved by Thermo Fisher Scientific Make s...

Page 9: ...k Group II according to the Laboratory Biosafety Manual of the World Health Organization WHO aerosol tight biological seals have to be used Look on the Internet page of the World Health Organization www who int for the Laboratory Biosafety Manual For materials in a higher risk group extra safety measures have to be taken If toxins or pathogenic substances have contaminated the centrifuge or its pa...

Page 10: ...trifuge is not aerosol tight In case of rotor failure the centrifuge can be damaged Leave the room Inform customer service CAUTION Never open the centrifuge door until the rotor has come to a complete stop and this has been confirmed in the display The emergency door release may be used in emergencies only to recover the samples from the centrifuge e g during a power failure Mechanical Emergency D...

Page 11: ...information on hazards described within the manual This symbol demands to disconnect mains before transporting or servicing the centrifuge This symbol demands to check if the rotor is installed correctly by lifting it slightly at the handle See Rotor Installation on page 25 Symbols used in the Manual This symbol refers to general hazards This symbol refers to biological hazards Observe the informa...

Page 12: ...dity 15 to 85 Permissible Ambient Temperature during Operation 2 C to 35 C Average Heat Dissipation 0 35 kWh 1170 Btu h 1140 kJ h Over voltage Category II Pollution Degree 2 IP 20 Running Time 99 h 59 min hold Maximum Speed nmax 17850 rpm Minimum Speed nmin 300 rpm Maximum RCF Value at nmax 30279 x g Noise Level at Maximum Speed1 55 dB A measured with TX 150 rotor Maximum Kinetic Energy 10 1 kJ Di...

Page 13: ...ng bucket rotor with carriers 75005706 M10 microplate swinging bucket rotor 75005723 M10 buckets 75005721 M10 sealed buckets 75005600 MT12 microtube swinging bucket rotor 75005709 HIGHConic III fixed angle rotor 75003623 CLINIConic fixed angle rotor 75005715 MicroClick 24x2 microtube rotor 75005719 MicroClick 30x2 microtube rotor 75003602 Microliter 48x2 sealed rotor 75005720 8x8 PCR Strip rotor 7...

Page 14: ... Machinery 2014 35 EU Low Voltage 2014 30 EU Electromagnetic Compatibility EMC EN 61010 1 EN 61010 2 020 EN 61010 2 101 EN 61326 2 6 EN 61326 1 Class B EN ISO 14971 EN ISO 13485 USA Canada 220 230 V 50 60 Hz 120 V 60 Hz FDA listed Product code JQC centrifuges for clinical use Device class 1 ANSI UL 61010 1 UL 61010 2 020 UL 61010 2 101 EN ISO 14971 EN ISO 13485 Japan 100 V 50 60 Hz China 230 V 50 ...

Page 15: ...o Scientific Sorvall ST 8FR centrifuge Article No 75007206 75007207 75007208 Mains Voltage 100 V 10 120 V 10 220 230 V 10 Frequency 50 60 Hz 60 Hz 50 60 Hz Rated Current 10 A 8 A 4 A Power Consumption 850 W 700 W 750 W Equipment Fuse 15 AT 15 AT 15 AT Building Fuse 15 AT 15 AT 16 AT Refrigerant R 134a R 134a R 134a Quantity 0 350 kg 0 350 kg 0 375 kg Pressure 26 bar 26 bar 21 bar GWP 1430 1430 143...

Page 16: ... items supplied are complete Items Supplied on page 6 If the items supplied are incomplete please contact Thermo Fisher Scientific 2 Location CAUTION UV rays reduce the stability of plastics Do not subject the centrifuge rotor and plastic accessories to direct sunlight The centrifuge is only to be operated indoors The set up location must fulfill the following requirements A safety zone of at leas...

Page 17: ...he centrifuge by its front panel its back panel or at its door NOTICE Always remove the rotor before moving the centrifuge If you do not remove the rotor you might damage the centrifuge drive or drive shaft Due to its weight the centrifuge must be carried by two people Always lift the centrifuge by using the handles Transport the centrifuge upright and with the centrifuge door closed 1 Lift the ce...

Page 18: ...Thermo Scientific Sorvall ST 8FR Centrifuge 18 Correct Incorrect 2 Move the centrifuge to the location where you want to operate it ...

Page 19: ...Turn off the power supply switch located on the right side when you stand before the centrifuge 2 Check whether the cable complies with the safety standards of your country 3 Make sure that the voltage and frequency correspond to the figures on the rating plate 4 Establish the connection to the power supply with the power supply cable ...

Page 20: ... leveling the centrifuge Level the centrifuge as follows 1 Open the centrifuge door See Open the Centrifuge Door on page 24 2 Put the bubble level near the arrow showing the rotation direction Centrifugation chamber Centrifuge desk centrifuge door open Arrow showing the rotation direction Bubble level 3 Adjust the centrifuge feet until the bubble in the level is fully in the circle mark To adjust ...

Page 21: ...astened against the centrifuge bottom If the centrifuge feet are not fastened imbalance can occur possibly causing a crash If 50 of the bubble stays in the circle mark the centrifuge is leveled If more than 50 of the bubble is out of the circle mark the centrifuge must be leveled again Excellent Acceptable Unacceptable ...

Page 22: ...o be thoroughly dried before storage Store the centrifuge in a clean dust free location Be sure to place the centrifuge on its feet Avoid storing the centrifuge in direct sunlight 7 Shipping WARNING Before shipping the centrifuge and accessories you have to clean and additionally disinfect or decontaminate the entire system if biological or chemical substances were used In doubt contact the Thermo...

Page 23: ...d depending on the used rotor Releasing the key initiates a stopping process according to the set acceleration and braking curve 7 OPEN Key Press the OPEN key to activate the automatic door release possible only if device is switched on and if the rotor is fully stopped Mechanical Emergency Door Release on page 47 8 STOP Key Press the STOP key to manually end the centrifugation run 9 START Key Pre...

Page 24: ...ped spinning The display shows the current speed also during a failure Never reach into the centrifugation chamber while the rotor is spinning CAUTION Injuries may occur from a declining centrifuge door gas spring If the pressure of the centrifuge door gas spring is not sufficient the centrifuge door will not stay open and can fall down Pay attention on the functionality of the centrifuge door gas...

Page 25: ...emergency lid lock Mechanical Emergency Door Release on page 47 3 Rotor Installation NOTICE Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge The approved rotors for the Thermo Scientific Sorvall ST 8FR are listed in section Available Thermo Scientific Rotors on page 13 Use only the rotors from this list in the centrifuge Permitted accessories are listed in ...

Page 26: ...entrifuge spindle NOTICE If the rotor cannot be properly locked in place after several attempts then the Auto Lock is defective and you are not permitted to operate the rotor Check for any damage to the rotor and the spindle Damaged rotors must not be used Keep the centrifuge spindle area of the rotor clear of objects If in doubt please call Thermo Fisher Scientific Customer Service NOTICE Check t...

Page 27: ...ifuge offers you 2 profiles standard and soft The setting is displayed above the Acceleration Deceleration Profiles key Press the Acceleration Deceleration Profiles key to cycle through and set the available profiles The LEDs show the chosen settings The last selected profile is saved and will be restored after a restart of the centrifuge LED Light Settings Description OFF Acceleration and Deceler...

Page 28: ...lue The relative centrifugal force RCF is given as a multiple of the force of gravity g It is a unit less numerical value which is used to compare the separation or sedimentation capacity of various centrifuges since it is independent of the type of device Only the centrifuging radius and the speed are used for calculation RCF 11 18 x x r r centrifuging radius in cm n rotational speed in rpm The m...

Page 29: ...you stop it manually Selecting the Temperature You can select temperatures between 10 C and 40 C To set the temperature proceed as follows Press the TEMPERATURE arrow keys This allows to change the set temperature using the arrow keys until the desired temperature is displayed Temperature will change in steps of single degrees Celsius Prewarming or Precooling the Centrifuge Ensure the rotor bucket...

Page 30: ...The centrifuge is able to save up to 99 programs It is only possible to save a program if the centrifuge is in standstill Loading or saving of programs is not possible if a rotor is spinning Saving a Program Modify the speed time and temperature to the desired setting For Direct Access Programs 1 2 3 Press and hold the desired program key 1 2 or 3 for 4 seconds For Programs 4 99 1 Press the folder...

Page 31: ... See User Menu on page 36 7 Centrifugation Maximum Loading The rotor can run at high speeds Each rotor is specifically designed to run at its maximum speed with a defined load The safety system of the centrifuge requires that you do not overload the rotor The rotor is designed to work with solution with a density of up to 1 2 g cm Above this density or if total load is above the maximum weight the...

Page 32: ... suitability Chemical Compatibility Chart on page 51 5 Make sure that the buckets are in correct position Set the parameters for the centrifugation For details Settings on page 27 Starting the Centrifugation Press the START key on the control panel The centrifuge accelerates to the set speed with the time display active If the speed setting is higher than the maximum permissible speed or RCF value...

Page 33: ...he run time is preset the centrifuge will run at the selected speed until the desired run time is reached It will then automatically decelerate and stop Once stopped RUN COMPLETED will be displayed and if selected the display will flash and the centrifuge will beep Access to the chamber and rotor can be gained by pressing the OPEN key If selected the door will open automatically You can also stop ...

Page 34: ... set values are restored NOTICE The centrifuge accelerates to maximum speed according to the rotor used Check carefully whether you have to maintain a certain speed for your application During the acceleration process time is counted forwards in seconds The reading stays displayed until the centrifuge door is opened 9 Removing the Rotor To remove the rotor proceed as follows 1 Open the centrifuge ...

Page 35: ...ied with a lid for aerosol tight applications come with a mandrel which belongs to the Auto Lock Be sure not to place the lid onto this mandrel to prevent it from being damaged For your protection when using an aerosol tight lid the rotor should only be removed with the lid closed 11 Switch off the Centrifuge To switch off the centrifuge push the mains switch to 0 ...

Page 36: ... SPEED arrow keys The shown entry can be changed using the TIME and TEMP arrow keys Press the START key to save this edit and quit the user menu Press the STOP key to quit the user menu Software ID and NVRAM ID information are entries within the user menu Values shown at some entries in the picture below are only examples NOTICE A total number of cycles completed on the centrifuge is counted since...

Page 37: ...p End of run flash Beeper volume Door Auto open LCD powersave Program only mode Software ID 0602 V01 NVRAM ID 4735 V05 Cycle count total 123456 Keypad beep YES or NO YES or NO YES or NO YES or NO 0 5 YES or NO YES or NO English Dutch Russian French Spanish Italian German ...

Page 38: ...t Use only cleaning agents that will not damage the equipment In doubt contact the manufacturer of the cleaning agent If in doubt contact Thermo Fisher Scientific Use warm water with a neutral detergent that is suitable for use with the materials If in doubt contact the manufacturer of the cleaning agents Never use caustic cleaning agents such as soap suds phosphoric acid bleaching solutions or sc...

Page 39: ...cleaning rotor and accessories they should be inspected for damage and wear Metal Parts Ensure that the black protective coating is complete It can be removed through wear and chemical attack and can lead to unseen corrosions Any signs of corrosions such as rust or white metallic pitting the rotor or accessories should be immediately removed from service Particular attention should be taken with t...

Page 40: ... water and a neutral detergent that is suitable for use with the materials If in doubt contact the manufacturer of the cleaning agents Clean away the grease from the rotor trunnions pivot point for swinging buckets 4 Use a soft brush without metal bristles to remove stubborn residue 5 Rinse rotor and accessories with distilled water 6 Place the rotor and buckets on a plastic grate with cavities po...

Page 41: ...ronmental conditions it may be necessary to clean it more often How to clean the filter mat 1 Remove the 4 screws holding the ventilation cover placed on the front of the centrifuge CAUTION Hold the ventilation cover with one hand while unscrewing it The ventilation cover is heavy and can cause minor injuries when falling down onto a hand or foot Remove the ventilation cover ...

Page 42: ...Thermo Scientific Sorvall ST 8FR Centrifuge 42 2 Remove the 4 screws holding the ventilation grid Remove the ventilation grid 3 Remove the filter mat ...

Page 43: ...o the centrifuge when a tube breaks or as a result of spills In case of contamination make sure that others are not put at risk Disinfect the affected parts immediately NOTICE Equipment can be damaged by inappropriate disinfection methods or agents Before using any cleaning or disinfection methods except those recommended by the manufacturer users should check with the manufacturer that the propos...

Page 44: ...d by inappropriate decontamination methods or agents Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Observe the safety precautions and handling instructions for the cleaning agents used After decontamination 1 Rinse the centrifuge and all affected acces...

Page 45: ...e it with distilled water Remove all accessories tubes adapters from the rotor Make sure that the necessary sterility is achieved according to your requirements 7 Maintenance Preventive Maintenance CAUTION Usage beyond the rotor and lid latch lifetime limits might lead to sample loss and damage to the centrifuge In order to keep this product able to perform the intended application in a reliable a...

Page 46: ...or free during the warranty period and afterwards for a charge This is only valid if the centrifuge has only been maintained by an authorized Thermo Fisher Scientific service technician 8 Shipping and Disposal WARNING When removing the centrifuge and accessories from use for disposal you have to clean and additionally disinfect or decontaminate the entire system if biological or chemical substance...

Page 47: ...d after the rotor has come to a complete stop Always wait until the rotor has come to a stop without braking The brake does not work when there is no current The braking process lasts much longer than usual Proceed as follows 1 Make sure the rotor has stopped view port in the centrifuge door 2 Pull out the power supply plug Keep the centrifuge horizontal at all times 3 On the right side of the hou...

Page 48: ...the centrifuge is accessible Be sure that the room temperature is within the limits Let the centrifuge cool down for 15 min Be sure there is no condensed water in the centrifugation chamber If the error message shows again contact the customer service E 017 E 020 E 021 E 022 E 023 E 078 E 079 E 080 E 081 Read Manual Wait until the rotor has stopped Check if the rotor is qualified for the centrifug...

Page 49: ...ilable Thermo Scientific Rotors on page 13 Restart the centrifuge If the error message shows again contact the customer service E 030 Power Failure Check the power supply of the centrifuge Make sure not to operate too many devices at one power source Let the centrifuge cool down for 15 min If the error message shows again contact the customer service E 098 Imbalance Load Check the load placed in t...

Page 50: ...o High The installed rotor is not rated for the programmed speed Check the programmed speed and the rotor 3 When to contact Customer Service If you need to contact the customer service please provide the order no and the serial no of your centrifuge This information can be found on the nameplate In addition the customer service also needs the software ID and the NVRAM ID Both are available in the ...

Page 51: ...er S tainless S teel T itanium T ygon V iton 2 mercaptoethanol S S U S M S S U S S U S S S S S S U S S S S S S A cetaldehyde S U U M U M U U U M M M S U S U A cetone M S U U S U M S S U U S U S U U U S S U U S M M S U U A cetonitrile S S U S M S S S U S U M U U S M U U S S S S U U A lconox U U S S S S S S S S S S M S S S S S S S S S S S U A llyl A lcohol U S S S S M S S S M S S A luminum C hloride...

Page 52: ... S U M S S S S S S S S S M S A mmonium H ydroxide conc U U U U S U M S S S U S U U S S S M S S S S U A mmonium P hosphate U S S S S S S S S S S S M S S S S S S M S S S A mmonium S ulfate U M S S S U S S S S S S S S S S S S S S S U S S U A myl A lcohol S M U S S M S M S S S S M U S M A niline S S U U S U S M S U U U U U U U S M U U S S S S U S S odium H ydroxide 1 U M S S S S M S S S M M S S S S S ...

Page 53: ... S S S S S M S S S C esium C hloride M S S U S S S S S S S S S S S S S S S S M S S S C esium F ormate M S S S S S S S S S S S S S S S S S S M S S S C esium I odide M S S S S S S S S S S S S S S S S S S M S S S C esium S ulfate M S S S S S S S S S S S S S S S S S S M S S S C hloroform U U U U S S M U S U U M U M U U U M M U U S U U U M S C hromic A cid 10 U U U S U U S S S U S S M U M S S U M S M U...

Page 54: ...S S S M U D iethyl K etone S U U M S U S M U U U M M U S S U U D iethylpyro carbonate S S U S S S S S U S U S U S S S M S S S S S S D imethylsulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U D ioxane M S U U S S M M S U U S U M U U M M M U S S S S U U F erric C hloride U U S M S M S S S S M U S S A cetic A cid G lacial S S U U S S U M S U S U U U U U M S U M U S U U S U A cetic A cid 5 S...

Page 55: ...S S S U S S S S S S S S M S M S E thylene O xide V apor S U U S U S S M S S S U S U S S S U F icoll H ypaque M S S S S S S S S S S S S S S S S S S M S S S H ydrofluoric A cid 10 U U U M U U U S S M U S S S S M S U U U H ydrofluoric A cid 50 U U U U U U U U U S U U U S S M M S U U U M H ydrochloric A cid conc U U U U U U M U M U U M U U U S U S U U U F ormaldehyde 40 M M M S S S S M S S S S M S S S...

Page 56: ... I odoacetic A cid S S M S S S S M S S M S S M S S S S S M S S M M P otassium B romide U S S S S S S S S S S S S S S S S S S S M S S S P otassium C arbonate M U S S S S S S S S S S S U S S S S S S S S S S S S P otassium C hloride U S S S S S S S S S S S S S S S S S S S S U S S S P otassium H ydroxide 5 U U S S S S M S S S S S U S S S S S S S M U M S U P otassium H ydroxide conc U U M U M M S S U M...

Page 57: ... S U M U S S M M S M M M M U S S A qua R egia U U U U U U U U U U U S M S olution 555 20 S S S S S S S S S S S S S S S S S S S S M agnesium C hloride M S S S S S S S S S S S S S S S S S S S S S M S S S M ercaptoacetic A cid U S U S M S S M S U U U U S U U S M S U S S S S M ethyl A lcohol S S S U S S M S S S S S U S U M S S S S S S S M S M U M ethylene C hloride U U U U M S S U S U U S U U U U U M ...

Page 58: ...S S U S odium B orate M S S S S S S S S S S U S S S S S S S S S S M S S S S odium B romide U S S S S S S S S S S S S S S S S S S S M S S S S odium C arbonate 2 M U S S S S S S S S S S S S U S S S S S S S S S S S S S odium D odecyl S ulfate S S S S S S S S S S S S S S S S S S S S S S S S S odium H ypochlorite 5 U U M S S M U S S M S S S M S S S S M S S S M U S M S S odium I odide M S S S S S S S S ...

Page 59: ...A cid S U S S S U U S U S S M S S S S S S S S S M U S M M O xalic A cid U U M S S S U S S S S S U S U S S S S S S S S U M S S P erchloric A cid 10 U U S U U S M M M U M S M M M S U S S P erchloric A cid 70 U U U U U S U M U U M U U U M M U M S U U S U S P henol 5 U S U S M M S U M U U S U M S M S U U S U M M M S P henol 50 U S U S U M S U M U U U U U S U M U U S U U U M S P hosphoric A cid 10 U U ...

Page 60: ... M S S S S S S S S S S S S S S S S S S S S S S S S S S ucrose A lkaline M S S S S S S S S S S S U S S S S S S S S M S S S S ulfosalicylic A cid U U S S S S S S S S U S S S S S S S S S U S S S N itric A cid 10 U S U S S U U S U S U S S S S S S S S S M S S S S N itric A cid 50 U S U M S U U S U S U U M M U M M M S S S U S S M S N itric A cid 95 U U U U U U U U U M U U U U M U U S U S S S H ydrochlor...

Page 61: ...U U M U U U U S U S S U U T oluene S S U U S S M U S U U S U U U S U M U U U S U S U U M T richloroacetic A cid U U U S S U M S U S U U S M M S S U U S U U U M U T richloroethane S U M U U S U U U U U U U U U S U S S T richloroethylene U U U U S U U U U U U U U U S U U S T risodium P hosphate S M S S S S S S S T ris B uffer neutral p H U S S S S S S S S S S S S S S S S S S S S S S S S S T riton X ...

Page 62: ...l T itanium T ygon V iton Z inc S ulfate U S S S S S S S S S S S S S S S S S S S S S S S S S C itric A cid 10 M S S M S S M S S S S S S S S S M S S S S S S S S S S Polyethlyeneterephtalate S Satisfactory M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc suggest testing under actual conditions of use U Unsatisfactory not recommended Perfo...

Page 63: ...Thermo Scientific Sorvall ST 8FR Centrifuge 63 ...

Page 64: ...Supplied 6 L Leveling 19 Location 16 M Mains Connection 19 Mains Supply 15 Maintenance and Care 38 Mechanical Emergency Door Release 47 N NVRAM ID 36 50 O Open the Centrifuge Door 24 Operation 24 P Precautions 6 Preface 5 R Refrigerant 15 Removing the Rotor 34 Rotor Installation 25 S Service of Thermo Fisher Scientific 46 Set Up 16 Shipping 22 46 Software ID 36 50 Storage 21 Switching on 24 Switch...

Page 65: ...re available in all countries Please consult your local sales representative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg 37520 Osterode am Harz Germany en Australia 61 39757 4300 Austria 43 1 801 40 0 Belgium 32 9 272 54 82 China 800 810 5118 400 650 5118 France...

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