9
Chapter 1: Introduction to the Multiskan FC
The Multiskan FC (Figure 1) is a high-quality filter-based microplate photometer. It is used to measure
absorbance from appropriate 96 or 384-well plate formats in the wavelength range of 340 to 850 nm.
The instrument allows shaking. The instrument is controlled through the built-in graphical user
interface and keypad. Multiskan FC can be connected to the SkanIt Software.
The Multiskan FC is available in the following configurations:
Multiskan FC
96-well plate reading with internal shaking
Multiskan FC with incubator
96 and 384-well plate reading with shaking and incubation
Figure 1.
Multiskan FC microplate photometer
Intended use
The Multiskan FC is a microplate photometer for measuring absorbance from suitable microplates
and strips in 96-well plate formats and optionally microplates in 384-well plate formats that meet the
ANSI/SBS standards.
The Multiskan FC, as standalone instrument or with SkanIt Software, can be used in research or
routine-test laboratories by professional personnel.
The Multiskan FC is part of the analyzing system used by the end user, who therefore is responsible
for validation of the whole system for reliable and safe results.
If the assay performance is essential for the analysis, the test result has to be ensured with internal
quality controls or with an alternative test.
It is recommended to use Good Laboratory Practice (GLP) during the analyzing process.
Principle of operation
The Multiskan FC is a filter-based microplate photometer. Measurement is vertical. The instrument
reads from bottom to top.
The optical system is based on one-channel optics with a tungsten halogen lamp as the light source
(Figure 2). Wavelength selection is made by means of interference filters.
