Thermo Scientific HERACELL VIOS 160i User Manual Download Page 72

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Operation

Preparing the device

5-2

HERA

CELL VIOS

160i

250i

Thermo Scientific

Fill volume for

HERA

CELL VIOS

160i

and

HERA

CELL VIOS

250i

: 3 l

Note

Hygiene regulations:

Prior to any operation, the user must clean and disinfect the workspace in accordance with the
hygiene regulations set forth by the operator to protect the cultures.

Water supply: see

“Relative humidity:“

page 3-7

When the filling level drops below a minimum value, it must be possible to top up water during the
work process.

Summary of Contents for HERACELL VIOS 160i

Page 1: ...50144132 A July 31 2015 Thermo Scientific HERACELL VIOS 160i HERACELL VIOS 250i CO2 Incubator User Manual ...

Page 2: ... of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposesonly System configurationsand specificationsin thisdocument supersede all previous information received by the purchaser Thermo Fisher Scientific Inc make...

Page 3: ...d purpose of the device 5 Correct use 5 Incorrect use 6 Standards and directives 6 Safety notes on gases 7 Instruction of the personnel 7 Safety notes on carbon dioxide CO2 7 Safety notes on oxygen O2 8 Safety notes on nitrogen N2 8 Chapter 1 Delivery of the Incubator 1 1 Packaging 1 1 Acceptance inspection 1 1 Standard equipment components HERACELL VIOS 160i 250i 1 2 Optional equipment components...

Page 4: ...ower supply connection 3 16 Workspace Components 3 17 Interior container 3 17 Glass door and optional gas tight screen 3 17 Water reservoir 3 19 Heating system 3 21 Rear panel openings 3 21 Shelf system 3 22 Chapter 4 Start up 4 1 Adapting the unit to the ambient atmosphere 4 1 Preparing the workspace 4 1 Installing the MAX fill level indicator and the pre filter 4 2 Install the air duct 4 4 Insta...

Page 5: ...ting the temperature set value 6 8 Setting the CO2 set value 6 9 Setting the O2 set value 6 10 auto start function 6 12 Activating the auto start routine 6 14 Interrupting the auto start routine 6 15 Launching steri run 6 16 User configuration 6 17 Settings Setup 6 17 Data Logging 6 27 Options 6 31 Icon description 6 40 Enabling disabling the keypad lock 6 42 Software versions 6 43 Trend display s...

Page 6: ...Installing the USB port driver 12 2 Structure of the command sequences for data communication 12 4 Description of protocol 12 4 Overview of general parameters addresses 0xxx 12 5 Reading general parameters 12 5 Overview of incubator parameters addresses 2xxx 12 5 Reading basic parameters 12 6 Reading internal function parameters 12 7 Error memory structure 12 8 Error memory data set structure sche...

Page 7: ...250i 5 Program HERACELL VIOS 160i HERACELL VIOS 250i 12 21 Installing the HERACELL VIOS 160i HERACELL VIOS 250i 12 22 Operating the HERACELL VIOS 160i HERACELL VIOS 250i 12 23 Chapter 13 Device log 13 1 Chapter 14 Contact Information Thermo Scientific 14 1 ...

Page 8: ...Table of Contents 6 HERACELL VIOS 160i 250i Thermo Scientific ...

Page 9: ...faces left side of control box 3 14 Figure 3 11 HERACELL VIOS 160i with glass door 3 18 Figure 3 12 HERACELL VIOS 160i with threefold gas tight screen 3 19 Figure 3 13 Water reservoir 3 20 Figure 3 14 Drain valve of water reservoir 3 20 Figure 3 15 Rear panel openings 3 21 Figure 3 16 Shelf system components 3 22 Figure 4 1 MAX fill level indicator 4 2 Figure 4 2 MAX fill level indicator and pre f...

Page 10: ... O2 display and O2 menu 6 11 Figure 6 12 Setting the O2 set value 6 11 Figure 6 13 Activating the auto start routine 6 14 Figure 6 14 auto start status display window 6 15 Figure 6 15 Interrupting the auto start routine 6 16 Figure 6 16 Error message subsequent to aborted auto start 6 16 Figure 6 17 User configuration menu 6 17 Figure 6 18 Settings Setup menu 6 18 Figure 6 19 Changing the keypad l...

Page 11: ...oncentration graph display 6 44 Figure 6 51 Event error messages 6 46 Figure 6 52 Failure message Overtemperature 6 46 Figure 6 53 Failure message Overtemperature 6 47 Figure 7 1 Drain valve of water reservoir 7 1 Figure 8 1 Drain valve of water reservoir 8 2 Figure 8 2 HEPA filter and airbox 8 5 Figure 8 3 Air duct 8 6 Figure 8 4 Decontamination routine phases 8 9 Figure 8 5 steri run menu Progre...

Page 12: ...Figures 4 HERACELL VIOS 160i 250i Thermo Scientific ...

Page 13: ...vice may present potential hazards particularly if it is operated by inadequately trained personnel or if it is not used in accordance with the intended purpose Therefore the following must be observed to prevent accidents The CO2 incubator must be operated only by trained and authorized personnel For any operation of this device the operator must prepare clear and concise written instructions in ...

Page 14: ...you encounter problems that are not detailed adequately in these operating instructions please contact Thermo Scientific immediately for your own safety Warranty Requirements for warranty Thermo Scientific warrants the operational safety and functions of the CO2 incubator only under the condition that the device is operated and serviced exclusively in accordance with its intended purpose and as de...

Page 15: ... in the operating instructions WARNING Indicates a hazardous situation which if not avoided could result in death or serious injuries CAUTION Indicates a hazardous situation which if not avoided could result in minor or moderate injuries CAUTION Indicates a situation which if not avoided could result in property damage Note Is used for application al hints and useful information ...

Page 16: ...mation and symbols 4 HERACELL VIOS 160i 250i Thermo Scientific Additional symbols for safety informations Wear safety gloves Wear safety goggles Harmful liquids Electric shock Hot surfaces Fire hazard Explosion hazard Suffocation hazard ...

Page 17: ...t end a controlled set of physiological ambient conditions is produced in the workspace of the device by exerting precise control of the following parameters Temperature CO2 content O2 N2 content Relative humidity CE conformity mark confirms conformity according to EU Guidelines TÜV tested safety Mark of conformity USA Canada EAC conformity mark confirms conformity according to all technical regul...

Page 18: ...ve release vapors that form combustible or explosive mixtures when exposed to air release poisons Standards and directives The device complies with the following standards and guidelines Low Voltage Directive 2006 95 EC IEC 61010 1 2010 Corr 2011 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements IEC 61010 2 010 2003 Safety requireme...

Page 19: ...ty instructions must be observed when the CO2 incubator is started up and when the device is operated Note Installation work Any work on supply lines and pressurized gas containers cylinders or containers used for storing CO2 or O2 N2 must only be carried out by expert personnel using the appropriate tools Note It must be ensured that the exposure limits at work place for CO2 and or O2 N2 are not ...

Page 20: ... panel and display window protective foil For cleaning these device components use only oil and grease free lubricants Keep all connections and components of the oxygen system free from substances that contain oil grease or lubricant Fire hazard Released oxygen O2 promotes combustion Do not use open flames in the vicinity of oxygen operated systems Do not smoke in the vicinity of oxygen systems Do...

Page 21: ...wood Acceptance inspection After the incubator has been delivered check the delivery immediately for completeness possible damage If components are missing or damage is found on the device or the packaging esp damages caused by humidity and water please contact the linehauler as well as the Technical Support of Thermo Scientific immediately Content Packaging on page 1 1 Acceptance inspection on pa...

Page 22: ...upport rails for shelves 4 Shelf support for inserts 6 Plug for access port 1 Power Cord 1 CO2 connecting hose set 1 User Manual 1 Quick release connector with hose for water draining 1 Components delivered with oxygen supply control option Pieces O2 sensor head 1 O2 connecting hose set 1 Components delivered with CO2 gas tank monitor option CO2 gas tank monitor connecting hose set 1 Components de...

Page 23: ...ponents HERACELL VIOS 250i Thermo Scientific HERACELL VIOS 160i 250i 1 3 Optional equipment components HERACELL VIOS 250i Components delivered with 250 liter units with split inserts Pieces Support brackets 3 Split inserts 6 Sixfold gas tight screen 1 ...

Page 24: ...1 Delivery of the Incubator Optional equipment components HERACELL VIOS 250i 1 4 HERACELL VIOS 160i 250i Thermo Scientific ...

Page 25: ...capable of bearing the weight of the device and of accessories particularly if several devices are stacked The electrical system of the device has been designed for an operating height of up to 2000 m above sea level To ensure a constant incubation temperature of 37 C the ambient temperature must be within a range of 18 C to 34 C Relative humidity up to 80 max No direct exposure to sunlight Device...

Page 26: ...e room climate Therefore the HERACELL VIOS 160i HERACELL VIOS 250i must only be installed in rooms with sufficient ventilation Do not install the device in room recesses without ventilation The room ventilation should be a technical ventilation that complies with the national requirements for laboritories or some other suited ventilation system with appropriate capacity Space requirements Figure 2...

Page 27: ...ts to be movable The rollers of the base rack are fitted with a locking brake Note Lift points Lift the device only using the lift points CAUTION Heavy loads Lift with care To avoid injury through physical strain such as strain traumata and slipped discs do not attempt to lift the incubator alone To avoid injury through dropped loads be sure to wear Personal Protection Equipment such as safety sho...

Page 28: ...device Stacking 2 4 HERACELL VIOS 160i 250i Thermo Scientific Figure 2 3 Stacking Note Moving stacked devices Stacked devices provided with a mobile base rack must only be moved inside rooms on even floors without any slopes ...

Page 29: ...r unit 8 Figure 2 5 3 On both sides align the bores in the connecting tab 9 Figure 2 5 of the adapter plate 7 Figure 2 5 with the bores in the rear wall of the lower unit 8 Figure 2 5 Figure 2 5 Fastening the adapter plate to the lower unit 4 Use the fastening screws supplied to bolt the adapter plate 7 Figure 2 5 to the rear wall of the lower unit 8 Figure 2 5 5 Fasten adapter plate 7 Figure 2 5 ...

Page 30: ...porting stacked devices Stacking elements are no connection elements Stacked devices provided with a mobile base rack must only be moved inside rooms on even floors without any slopes Note Fastening on mobile racks If the devices are installed onto mobile racks make sure that the rollers are secured with a locking brake during the operation of the incubators and that the rollers are oriented towar...

Page 31: ...L VIOS 160i VIOS 250i Front view on page 3 2 HERACELL VIOS 160i VIOS 250i Rear view on page 3 4 Safety devices HERACELL VIOS 160i VIOS 250i on page 3 6 Workspace atmosphere on page 3 6 Door switch on page 3 10 Sensor system on page 3 11 Supply interface on page 3 13 Workspace Components on page 3 17 ...

Page 32: ...S 160i VIOS 250i Front view 3 2 HERACELL VIOS 160i 250i Thermo Scientific HERACELL VIOS 160i VIOS 250i Front view Figure 3 1 HERA CELL VIOS 160i VIOS 250i Front view 1 18 4 3 2 8 6 5 7 19 20 21 16 14a b 17 15 13 10 9 11 4 9 16 10 9 12 14a 14b ...

Page 33: ...6 Glass door or gas tight screen optional 7 Outer door 8 Magnetic door seal replaceable 9 Prefilter 10 Water drain 11 Stand height adjustable 12 Glass door seal replaceable 13 Airbox with seal and HEPA filter 14 a Bottom insert standard feature set b Bottom insert with cut out for refilling optional only for HERA CELL VIOS 160i 15 Support rail 16 Insert with shelf support 17 rH sensor with TCD sen...

Page 34: ...and O2 N2 gas connection without gas monitoring 3 O2 N2 gas cylinder 4 CO2 gas cylinder 5 Primary CO2 gas supply A with optional gas monitoring 6 Secondary CO2 gas supply B with optional gas monitoring 7 Control box with supply interface for combined gas connection optional CO2 and O2 N2 with gas monitoring optional 8 Primary O2 N2 gas supply A with optional gas monitoring 9 Secondary O2 N2 gas su...

Page 35: ...RACELL VIOS 160i HERACELL VIOS 250i Figure 3 3 Looped through gas supply HERACELL VIOS 160i HERACELL VIOS 250i 1 Control box with supply interface for combined gas connection optional CO2 and O2 N2 with gas monitoring optional 2 Distributor connection for looping through the CO2 gas supply for up to three additional devices independent of device type 3 Distributor connection hidden for looping thr...

Page 36: ...ilures A pressure compensation opening ensures pressure compensation in the device workspace The alarm relay circuit uses audible and visual alarms to indicate errors during operation Workspace atmosphere In the workspace of the incubator the particular physiological ambient conditions for the preparation and cultivation of cell and tissue cultures are simulated The workspace atmosphere is determi...

Page 37: ...in the workspace can be set to a lower level The relative humidity in the workspace is lowered from approx 93 to approx 90 The modification requires an extended adaption phase To ensure that it effectively prevents dew formation on culture containers it must be used as a permanent setting Instructions for activating the Low Humidity function are given in section Low humidity setting on page 6 33 P...

Page 38: ...the airbox 1 Figure 3 4 from below The airbox rests on the water reservoir cover panel 2 Figure 4 5 and is pushed against the air intake ç Figure 3 4 HEPA filter and airbox Instructions for activating the HEPA filter monitor are given in section Activating de activating the HEPA filter on page 6 39 The air duct feeds the flow of aire from the fan along the rear wall 3 Figure 3 5 to the workspace c...

Page 39: ...f the bicarbonate buffered culture media largely depends on the CO2 content of the workspace atmosphere The CO2 content of the workspace atmosphere can be controlled within a range of 0 20 The supplied CO2 must have one of the following quality characteristics Purity 99 5 min medical gas quality O2 supply If the CO2 incubator is to be operated with more than 21 oxygen the workspace is supplied wit...

Page 40: ...pper edge of the workspace opening When the door switch is activated by opening the glass door the gas supply and workspace heating are suspended The display shows a corresponding message An alarm sounds if the door is open longer than 30 seconds If the door remains open for more than 10 minutes an audible alarm sounds and the alarm relay responds The outer door can only be closed after the glass ...

Page 41: ...the workspace atmosphere 3 Figure 3 7 Depending on the configuration a TCD sensor or IR sensor option is mounted in this location rH sensor for monitoring the humidity of the workspace atmosphere 4 Figure 3 7 The rH sensor option teams up with the TCD sensor to provide for humidity compensation It is not present on units operated with an IR sensor Figure 3 7 Temperature O2 CO2 and humidity sensors...

Page 42: ...temperature distribution throughout the workspace The overtemperature protection feature is factory preprogrammed and requires specially trained service personnel in case changes are needed It protects the stored cultures from overheating If the set temperature is exceeded by more than 1 C the thermal protection responds and the workspace temperature is automatically reduced to the selected set va...

Page 43: ...3 Description of the device Supply interface Thermo Scientific HERACELL VIOS 160i 250i 3 13 Supply interface Standard interfaces Figure 3 9 Interfaces right side of control box CO2 O2 ...

Page 44: ...2 connector not available with CO2 and O2 N2gas supply sources and simultaneous use of gas monitoring option 3 CO2 connector 4 Label 5 USB interface 6 Alarm contact 7 Power supply connection Optional interfaces Figure 3 10 Optional interfaces left side of control box The connectors on the left side Figure 3 10 are only available with units equipped with the respective options 1 four connectors to ...

Page 45: ...onnection USB 1 1 USB 2 0 USB 3 0 full speed compatible allows the fast and temporary acquisition of the major operating parameters temperature CO2 O2 N2 concentration error codes etc 4 20 mA interface The optional 4 20 mA interface 2 Figure 3 10 converts the digital actual values displayed for temperature CO2 concentration O2 concentration optional and relative humidity into a continuous output c...

Page 46: ... unit 6 Figure 3 9 Power supply connection Mains power is connected to the unit using a cable with an IEC connector plugged into the IEC socket in the control box 7 Figure 3 9 The mains socket must be easily identifiable by the user and freely accessible at all times Current Significance 4 mA The measured value is smaller than or equal to the lower boundary of the measurement range 20 mA The measu...

Page 47: ... of the interior container workspace components such as the air duct and shelving is also made of the same stainless steel or copper material The airbox for the HEPA filter is made of thermally resistant plastics material and must remain installed while the Steri Run decontamination routine is running The shelving system components the airbox the air duct and the cover of the water reservoir can b...

Page 48: ...s devices that are equipped with the optional gas tight screen have a considerably lower contamination hazard and the recovery times of the incubation parameters are shorter for workspace temperature CO2 concentration O2 N2 concentration relative humidity Gas tight screens are available as options for both types of products HERACELL VIOS 160i threefold gas tight screen see Figure 3 12 HERACELL VIO...

Page 49: ...r reservoir allows for quickly draining the water through a drain valve 3 Figure 3 13 located at the front section of the device Placed on the front section of the water reservoir cover is a pre filter 4 Figure 3 13 The pre filter consists of a dual layer wire mesh with a silicone frame and is autoclavable as well as heat resistant The pre filter must remain installed in the device during the Ster...

Page 50: ... MAX 1 Figure 3 13 is suspended above the water reservoir to indicate the maximum fill level The maximum fill volume of the water reservoir is 3 liters In order to minimize interference to the workspace atmosphere during water replacement without disruption of incubation operation the unit has a front side quick drain valve Plugging the drain tube supplied into the front side quick drain valve sta...

Page 51: ...nterior glass door gas tight screen prevents condensate formation The workspace of the device always remains visible despite high humidity Rear panel openings Figure 3 15 Rear panel openings The access port with insert Figure 3 15 Ø 42 mm 1 66 inch can be sealed with a plug 1 Figure 3 15 and allows for feeding cables tubes or additional sensors into the workspace of the unit The pressure compensat...

Page 52: ...ide in Figure 3 16 HERACELL VIOS 160i with a cutout for refilling 4 Figure 3 16 For details on using the shelf system see Start up on page 4 1 Note Operating conditions When accessories are to be operated in the workspace of the CO2 incubator the ambient condition requirements must be observed see table The energy introduced into the workspace affects the beginning of the temperature control range...

Page 53: ... page 4 1 Installing the MAX fill level indicator and the pre filter on page 4 2 Install the air duct on page 4 4 Installing the shelf system on page 4 7 Mount the split inserts optional into the HERACELL VIOS 250i on page 4 9 Gas connection on page 4 10 Power supply connection on page 4 15 Connecting the USB interface on page 4 16 Connecting the alarm contact on page 4 16 CAUTION Adapting the uni...

Page 54: ... sure that the tube is removed from the drain valve 3 Figure 4 2 2 Check whether the drain 2 Figure 4 2 in the front section of the water reservoir is free of obstruction it is needed for discharging the water through the drain valve 3 Figure 4 2 on the front side of the unit 3 Suspend the MAX fill level indicator 1 Figure 4 1 in the slot provided in the water reservoir cover panel 5 Figure 4 1 Fi...

Page 55: ...MAX fill level indicator and the pre filter Thermo Scientific HERACELL VIOS 160i 250i 4 3 4 Insert the pre filter 4 Figure 4 2 into the water reservoir cover panel Figure 4 2 MAX fill level indicator and pre filter 1 2 3 4 2 2 1 ...

Page 56: ...ure that in step C of Figure 4 3 the positioning tab at the rear part clicks into place in the rectangular hole of the bottom part 2 Place the tab at the bottom of the rear part 2 Figure 4 3 on the two rear wall studs and tilt the air duct backwards 3 Lock the lateral keyholes of the top part step G Figure 4 3 into the retaining screws in the workspace ceiling Figure 4 3 Assembling the air duct B ...

Page 57: ... pushed against the air intake Figure 4 4 Assembling the HEPA filter and the airbox 1 Place the HEPA filter 2 Figure 4 4 on a flat surface 2 Tilt the airbox 1 Figure 4 4 to the left and insert the tabs on the left 3 Figure 4 4 into the corresponding slots provided in the HEPA filter 4 Figure 4 4 3 Insert the tabs 5 Figure 4 4 on the right side of the airbox into the HEPA filter catches 6 Figure 4 ...

Page 58: ...nel on the bottom of the workspace 7 Lift the water reservoir cover at the front side and push it towards the rear wall 1 Figure 4 6 Figure 4 6 Installing the airbox 8 Push the water reservoir cover panel all the way towards the rear wall Doing so lets the cover panel slide into its final position in the reservoir and the tube end of the airbox into the fan outlet 9 Let the front edge of the water...

Page 59: ...to the rail the shelves are pushed onto the support hooks Installation removal of the support rail The support rails are guided and held in place by the embossings The locksprings of the support rails must point upwards 1 Position the support rail onto the lower embossing and tilt it toward the workspace side wall so that the rail is positioned over the two embossings 2 Clamp the lockspring behind...

Page 60: ...es tilt with the protection device 5 Figure 4 8 ahead onto the shelf support The tilt protection is also used as a guide for the shelf 2 Slightly raise the shelf so that the withdrawal stop 6 Figure 4 8 can be routed over the shelf supports 3 Make sure that the shelf support is positioned in the two tilt protections in a way that allows it to move freely Levelling the device 1 Position a bubble le...

Page 61: ...to front Mount the split inserts optional into the HERACELL VIOS 250i Figure 4 9 Installation of split inserts For a HERACELL VIOS 250i equipped with an optional six fold gas screen and 6 segment split inserts three support frames with two shelves each are placed onto the shelf supports of the lateral support rails instead of the one piece shelves of the standard version 1 Place both these shelves...

Page 62: ...p 1 Figure 4 10 onto the gas pressure hose 2 Figure 4 10 and connect hose to the sleeve 4 Figure 4 10 of the sterile filter 5 Figure 4 10 Note Gas quality The gases must have one of the following qualities Purity 99 5 min medical gas quality CAUTION Overpressure The operating pressure of the gas applied to the device must not exceed 1 bar 14 5 psi If the gas is supplied at a higher pressure the va...

Page 63: ...hose clamp Gas connection without gas monitoring system Figure 4 11 Gas connection without gas monitoring system Note Pressure compensation opening To ensure permanent pressure compensation the pressure compensation opening must not be connected to an exhaust air system The pipe of the pressure compensation opening must not be extended or redirected ...

Page 64: ...nal On units with a combined CO2 O2 N2 connection the gas supply tubes must be routed according to the following connection diagram 3 Figure 4 11 Connect the O2 N2 supply line to the upper gas inlet filter 4 Figure 4 11 connect the CO2 supply line to the lower gas inlet filter 5 Figure 4 11 CO2 connection with gas monitoring system optional Figure 4 12 CO2 connection with gas monitoring system opt...

Page 65: ... three additional devices For looping the gas supply through the devices must be interconnected with a gas pressure hose Item 2 Figure 4 12 Connect the gas container B to the upper connection of the gas monitoring system Item 3 Figure 4 12 Connect the gas container A to the lower connection of the gas monitoring system Items 4 5 Figure 4 12 The outlet of the gas monitoring system is connected at t...

Page 66: ...nection of the gas monitoring system Item 1 Figure 4 13 The outlet of the gas monitoring system is connected at the factory to the gas inlet filter item 8 Figure 4 13 by a short gas pressure hose CO2 supply Item 4 Distributor connection for the CO Figure 4 132 gas supply for up to three additional devices For looping the gas supply through the devices must be interconnected with a gas pressure hos...

Page 67: ...st not be connected to the power supply 2 Connect the IEC connector to the socket of the control box of the device 3 Connect the grounding plug of the power supply cable to a correctly grounded and fused socket 4 Make sure the power supply cable is not subjected to tensile or compressive force 5 Mains connection The mains socket must be easily identifiable by the user and freely accessible at all ...

Page 68: ...0 Baud Baud rate adjustment is described in section USB interface baud rate setting on page 6 23 Connecting the alarm contact Function When system errors and failures occur in the temperature or gas control circuits an alarm message is issued to the connected alarm monitoring system The potential free contact 1 changeover contact has been designed for the following circuits Alarm relay Note Skille...

Page 69: ... voltage and the fusing of external alarm circuitry are given in the table above 1 Wire the individual conductors as shown in the wiring diagram 2 Connect the connector of the alarm system connecting cable to the interface at the control box at the rear panel of the device Error power failure ON X o Key X Contact closed O Contact open Note Switching structure The alarm relay switches for all failu...

Page 70: ...trofitting of the 4 20 mA data interface is reserved exclusively to Thermo Fisher Scientific Technical Field Service staff Note Skilled work Thermo Scientific warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly The connection of the device to an external alarm system must only be carried out by adequately trained and authorize...

Page 71: ...pressure compensation opening must be permeable its insert must be installed in the workspace The glass door seal must not be damaged The glass door gas tight screen measurement opening must be capped The shelf system components must be installed safely The airbox with the HEPA filter and the air duct must be installed according to the instructions Decontaminating the device workspace Run the ster...

Page 72: ...te Hygiene regulations Prior to any operation the user must clean and disinfect the workspace in accordance with the hygiene regulations set forth by the operator to protect the cultures Water supply see Relative humidity on page 3 7 When the filling level drops below a minimum value it must be possible to top up water during the work process ...

Page 73: ... 2 Figure 5 1 3 Insert with cut out for refilling HERACELL VIOS 160i Open flap 3 Figure 5 1 Single piece insert HERACELL VIOS 250i Remove bottom insert if there is not enough space for the container used for filling 4 Fill reservoir with sufficiently purified water beneath cover item 1 in Figure 5 1 of the workspace Figure 5 1 Water reservoir 1 2 1 2 3 ...

Page 74: ...1 Adjust set values for temperature and CO2 O2 concentration on the iCan touchscreen Starting the device 12 Start the device with the auto start routine see Activating the auto start routine on page 6 14 13 The auto start progress indicator appears on the display and the automatic start routine is run 14 The temperature control adjusts the temperature to the selected set value humidity rises 15 Wh...

Page 75: ...and when the ambient temperature is low the auto start routine may take up to 10 hours Loading To ensure sufficient air circulation and even heating of the samples the loading surface within the workspace should be used up to 70 max Bulky objects in the workspace that dissipate heat may impair heat distribution Bulky objects in the use able space that dissipate heat may impair heat distribution ...

Page 76: ...5 Operation Starting operation 5 6 HERACELL VIOS 160i 250i Thermo Scientific ...

Page 77: ... 7 Heat up phase of the control loop sensors on page 6 7 Behavior of keys during adjustments on page 6 8 Setting the temperature set value on page 6 8 Setting the CO2 set value on page 6 9 Setting the O2 set value on page 6 10 auto start function on page 6 12 Launching steri run on page 6 16 User configuration on page 6 17 Trend display scaling on page 6 43 Error messages on page 6 45 Measures aft...

Page 78: ... in the side panel of the unit Powering up Press the power switch the switch illumination comes on After a short beep the blank screen vanishes and the touchscreen is displayed The control loop sensors pass the heat up phase Heat up phase of the control loop sensors on page 6 7 Powering down Press the power switch the switch illumination goes off ...

Page 79: ...nsitive screen areas The following screen areas of the operating panel are touch sensitive and accept operating commands Temperature display T CO2 display CO2 Display field water level RH O2 display optional Icon bar with symbolic representations of operating states allowing direct access to installed options see also Icon description on page 6 40 steri run key Menu key auto start key Note Extende...

Page 80: ... with actual value large numeric readout at center set value small numeric readout top right and physical unit bottom right 2 CO2 concentration display field with actual value large numeric readout at center set value small numeric readout top right and physical unit bottom right 3 HEPA filter activity display icons for further options see Icon description on page 6 40 4 Key for starting the steri...

Page 81: ...dout at center set value small numeric readout top right and unit bottom right 3 HEPA filter activity display icons for further options see Icon description on page 6 40 4 Key for starting the steri run decontamination routine 5 Key for opening the menu navigation 6 Key for starting the auto start routine 7 O2 concentration display field with actual value large numeric readout at center set value ...

Page 82: ...6 Handling and control Operating panel and operating structure 6 6 HERACELL VIOS 160i 250i Thermo Scientific Figure 6 5 Menu structure overview A B C ...

Page 83: ...ing duration during the start process 1 Temperature control loop 10 s 2 CO2 sensor heat up period TCD and IR 5 min 3 O2 control loop 5 min Figure 6 6 Heat up phase display The start process is indicated by an audible signal During the heat up phase the displays show dots instead of values Temperature display CO2 display and O2 display field Note CO2 O2 control Since the CO2 concentration of the ai...

Page 84: ...emperature key The Temperature menu Figure 6 7 is displayed Figure 6 7 Temperature display field and temperature selection menu 2 To exit the Temperature menu Press the End key 3 3 To set the temperature set value Press the Set value key Note CO2 gas supply During the 5 minute heat up phase of the O2 control circuit the CO2 supply to the workspace and monitoring of the CO2 control circuit are susp...

Page 85: ... key To reduce the set value Press the key 4 To accept and save the set value Press the Enter key The system returns to the main menu The temperature displays shows the actual value currently measured in the workspace Setting the CO2 set value 1 Press the CO2 display key The CO2 menu is displayed Figure 6 9 CO2 display and CO2 menu 2 To exit the CO2 menu Press the End key 3 ...

Page 86: ...the workspace Figure 6 10 Setting the CO2 set value Setting the O2 set value This setting is possible only on versions with the optional O2 N2 control 1 Press the O2 display key The O2 menu is displayed Note Deactivating the CO2 control loop To deactivate the CO2 control the set value is set to 0 If the control loop is deactivated error monitoring is enabled as well Gas monitoring The switching st...

Page 87: ...nu Press the End key 3 To set the O2 set value Press the Set value key To increase the set value Press the key To reduce the set value Press the key 4 To accept and save the set value Press the Enter key The system returns to the main menu The O2 display shows the actual value currently measured in the workspace Figure 6 12 Setting the O2 set value ...

Page 88: ...O2 control ranges has been preset at the factory Control range I 1 21 Control range II 5 90 Usage of the process gases For set values below 21 O2 the device must be connected to a nitrogen supply system For set values above 21 O2 the device must be connected to an oxygen supply system For set values of 21 O2 the O2 control is switched off and error monitoring is disabled as well The control loop e...

Page 89: ...is opened or if the power supply of the device is interrupted while the auto start routine is running the routine is interrupted and rerun after the glass door has been closed or the power supply has been reestablished Start conditions for the auto start routine Prior to running the auto start routine set the CO2 and O2 set values to the desired values and make sure that the atmosphere in the work...

Page 90: ...n menu and stop auto start Press the End key 3 3 Refill water if necessary 4 Activate the auto start routine Press the Start key 5 To air the workspace open both device doors When the audible alarm sounds after 30 seconds Auto start routine aborted due to error The auto start routine is aborted if an error is detected in the temperature control loop an error is sensed in the CO2 control circuit th...

Page 91: ...ently cancelled or resumed 1 To resume the auto start routine Press the Back key The system returns to the status display and the auto start routine is resumed 2 To cancel the auto start routine Press the End key 3 The warning triangle is displayed as a failure message together with the audible signal Note Cancellation The auto start routine can be cancelled at any time Press the Stop key Automati...

Page 92: ...ialog box with the description of the error is displayed Figure 6 16 Error message subsequent to aborted auto start 4 To accept the failure message Press the End key 3 The system returns to the main menu Launching steri run The steri run decontamination routine is used to decontaminate the complete workspace An in depth description of the function is given in the chapter Cleaning and disinfection ...

Page 93: ... user configuration menu is split into six categories Settings Event logging Options Icon description Keypad lock Software versions To make a user specific setting in a dialog box navigate through the submenus listed in the illustrations and open the dialog box Settings Setup The Settings selection menu Figure 6 18 allows for accessing a series of dialog windows with adjustment options for customi...

Page 94: ...numbers Upon delivery the default code is 0000 This default can be changed into a user defined code that is then enabled using the KEYPAD LOCK dialog box Enabling disabling the keypad lock on page 6 42 Changing the keypad lock code 1 Press the Menu key 2 Select the menu command Keypad lock code The input dialog shown in Figure 6 19 is displayed Figure 6 19 Changing the keypad lock code 3 To enter ...

Page 95: ... Backspace key 6 To accept and save the input value Press the Enter key The system returns to the Settings Setup menu A padlock icon appears on the Icon bar in the main menu Figure 6 2 on page 3 as an activity indicator for the keypad lock Date time setting The input dialog allows date and time to be set to the required time zone 1 Press the Menu key 2 Select the menu command Date Time The selecti...

Page 96: ...displayed Figure 6 22 Setting the date 4 To enter the date Press the numeric keys The input numbers are displayed in the input box 5 To set the cursor to the left to overwrite a value Press the Backspace key 6 To accept and save the input value Press the Enter key 7 The system returns to the Date Time menu 8 For changing the time choose the Time option The input dialog shown in Figure 6 23 is disp...

Page 97: ...overwrite a value Press the Backspace key 11 To accept and save the input value Press the Enter key 12 The system returns to the Date Time menu Adjusting display brightness The selection menu allows for adjusting the brightness of the operating panel over a range from 1 to 100 Setting the contrast 1 Press the Menu key 2 Select the menu command Settings Setup 3 Select the option Display contrast Th...

Page 98: ...on has been changed but not saved yet 7 To accept and save the change Press the Enter key The system returns to the Settings Setup menu Key tone setting The input dialog allows the loudness of the key tone that sounds whenever a key is depressed to be set The value range is 0 to 100 The change occurs in increments of 5 Adjusting key tone volume 1 Press the Menu key 2 Select the menu command Settin...

Page 99: ...selection has been changed but not saved yet 4 To accept and save the change Press the Enter key The system returns to the Settings Setup menu USB interface baud rate setting This selection menu allows for adjusting the data rate of the USB interface The baud rate can be changed within the defined baud rates 9 600 19 200 38 400 57 600 baud Setting the baud rate 1 Press the Menu key 2 Select the me...

Page 100: ... of the USB interface Figure 6 27 To increase the baud rate Press the key To reduce the baud rate Press the key 5 The value change appears in the display The New message indicates that the selection has been changed but not saved yet 6 To accept and save the change Press the Enter key The display returns to Interface USB selection menu 7 To activate the new settings Browse back to the main menu Wa...

Page 101: ...lecting the display language 1 Press the Menu key 2 Select the menu command Language The input dialog shown in Figure 6 28 is displayed Figure 6 28 Language setting 3 To browse upward in the selection Press the key 4 To browse downward in the selection Press the key 5 The new language appears in the display The New message indicates that the selection has been changed but not saved yet 6 To accept...

Page 102: ...ase run Steri Run routine auto start Please run auto start routine Appears upon successful completion of the Steri Run decontamination routine Service interval Request service The service message can be confirmed In this case the Request Service icon is displayed After the routines have been run successfully the reminder messages are hidden Factory settings Steri run decontamination routine 90 day...

Page 103: ... New message indicates that the selection has been changed but not saved yet 4 To deactivate the reminder interval Set the value to Off Press the key 5 To accept and save the change Press the Enter key The display returns to the reminder intervals selection menu Data Logging The Event logging selection menu Figure 6 31 allows for accessing dialog screens for logging and displaying events that occu...

Page 104: ...with the most recent event at the top position The list can be displayed but not edited If the event display consists of several pages the user can browse through the list The status indicator indicates which page of the total number of pages is currently being displayed Invoking the event log 1 Press the Menu key 2 Select the menu command Event logging The selection dialog shown in Figure 6 31 is...

Page 105: ...ging the logging cycle time Due to limited memory resources the oldest entries are deleted as new entries are logged The period from which the displayed entries originate depends largely on the selected logging cycle time Invoking the event log 1 Press the Menu key 2 Select the menu command Event logging The selection dialog shown in Figure 6 31 is displayed 3 Select the menu command Recording int...

Page 106: ...vice integral monitoring system in descending chronological order The most recently detected error is listed at the top position of 22 possible entries An entry consists of the control loop in which the error occurred the date the time and an error description The error table can be displayed but not edited If the event display consists of two pages the user can browse through the list The status ...

Page 107: ... Exit the display Press the End key 3 The system returns to the Event logging menu Options The Options selection menu Figure 6 35 allows for accessing all selection dialogs for the functional options of the unit Alarm Low humidity Gas tight screen optional Humidity sensor Audible alarm O2 gas supply optional HEPA filter Note Troubleshooting For a detailed overview of causes for errors and their co...

Page 108: ...ystem network monitoring can be enabled or disabled If network monitoring is enabled a power failure is detected as an error Wiring details for the alarm relay are described in the section Connecting the alarm contact on page 4 16 1 Press the Menu key 2 Select the menu command Options The selection dialog shown in Figure 6 35 is displayed 3 Select the menu command Alarm The selection dialog shown ...

Page 109: ...pace can be set to a lower level At the factory the device control is preset to high humidity approx 93 relative humidity The relative humidity in the workspace is lowered from approx 93 to approx 90 The modification requires an extended adaption phase To ensure that it effectively prevents dew formation on culture containers it must be used as a permanent setting Reducing humidity in the workspac...

Page 110: ...s tight screen achieve shorter recovery times for the incubation parameters Workspace temperature CO2 concentration O2 concentration relative humidity After conversion of the device the device control system must be adjusted to the gas tight screen option Gas tight screen setting 1 Press the Menu key 2 Select the menu command Options Note Low humidity The enabling disabling of the Low humidity fun...

Page 111: ...on with ambient humidity or if the auto start routine is to be run dry without water the water level sensor can be switched off This prevents alarm messages for the water level sensor by the device integral monitoring system from being issued Activating de activating the water level sensor 1 Press the Menu key 2 Select the menu command Options The selection dialog shown in Figure 6 35 is displayed...

Page 112: ...ons menu Switching the audible alarm on off If the device integral monitoring system detects an error an audible alarm sounds in addition to the visual error message and the switching of the alarm relay The audible alarm can be permanently disabled Switching the audible alarm on off 1 Press the Menu key 2 Select the menu command Options The selection dialog shown in Figure 6 35 is displayed 3 Sele...

Page 113: ...ns to the Options menu Switching the O 2 control on and off Depending on the requirements to the work process the O2 control can be switched on and off This setting is possible only on versions with the optional O2 N2 control Switching the O 2 control on and off 1 Press the Menu key 2 Select the menu command Options The selection dialog shown in Figure 6 35 is displayed 3 Select the menu command O...

Page 114: ... or N2 supply the workspace must be vented after de activating O2 control Gas monitoring The switching state of the O2 control loop does not affect the function of the optional gas monitoring system The gas monitoring system remains active even when the O2control is switched off or the O2 sensor is disabled Note Display of O2 value When O2 control is de activated the actual value readout shows thr...

Page 115: ...ions Activating de activating the HEPA filter 1 Press the Menu key 2 Select the menu command Options The selection dialog shown in Figure 6 35 is displayed 3 Select the menu command HEPA The selection dialog shown in Figure 6 43 is displayed Figure 6 43 HEPA configuration 4 Select the menu command HEPA configuration The input dialog shown in Figure 6 44 is displayed Figure 6 44 Activating de activ...

Page 116: ...dition to the entries in the event or in the error table The Icon description dialog box explains the meaning of the individual icons Invoking the icon description 1 Press the Menu key 2 Select the menu command Icon description The information dialog shown in Figure 6 45 is displayed Figure 6 45 Icon description Exit the display Press the End key 3 The system returns to the User configuration menu...

Page 117: ...ator showing the enabled state of the HEPA filter in the workspace see Activating de activating the HEPA filter on page 6 39 for instructions Request service This indicator shows that the routine service is due The display of the icon is controlled by the time entry in the Reminder Interval dialog box and it appears after the reminder message has been confirmed Gas cylinder filling level display o...

Page 118: ...r table an entry is written into the event list Enabling disabling the keypad lock This input dialog box allows the keypad lock to be enabled or disabled At the factory the keypad lock is preset to the standard code 0000 1 Enter the 4 digit code using the keypad The input appears encrypted in the display 2 To delete complete incorrect input Press the Delete key 3 To break off the input Press the B...

Page 119: ...shows the unit software versions in the display Exit the display Press the End key 3 The system returns to the User configuration menu Figure 6 48 Software versions Trend display scaling The trend display of the three control loops temperature 0 20 CO2 0 100 O2 can be scaled to two different versions 1 Full screen display Press the CO2 display field in the main menu ...

Page 120: ... dragging a diagonal from the start press onto screen at left of upper diagram frame to the end release at right of lower diagram frame Press any position within the marked rectangle area The section is now enlarged This may be repeated as often as necessary until the section of the graph is shown at the desired magnification or until the maximum magnification max 30 data logger elements equivalen...

Page 121: ...knowledged that error can be redisplayed by pressing a red key T CO2 O2 RH or system Menu key Response to an error message event If the alarm relay was switched due to a user action the switching state can be reset by accepting the error message e g for the manual cancellation of the steri run decontamination routine If the alarm relay was switched due to a technical defect the switching state rem...

Page 122: ... unit has activated overtemperature protection and switched over to emergency control mode the main menu is overlaid by a flashing warning triangle and the pertinent icon Figure 6 52 Failure message Overtemperature 1 Displaying the cause of an error Press anywhere on the touchscreen The Error dialog box appears and the detected error is displayed The audible alarm is switched off ...

Page 123: ...utage the formation of condensation can occur at the sensors due to moisture This can impair the sensor function and lead to erroneous values being displayed or to failure messages sensor breakage see Troubleshooting on page 6 48 The following measures must be taken to ensure safe operation 1 Drain the water and wipe the interior dry 2 Let the device warm up without water for 1 hour to 55 C 3 Then...

Page 124: ...ective Reset unit If the error occurs repeatedly call service X X X Error data logger Error while writing to the memory of the data logger Incubator is still operative Reset unit If the error occurs repeatedly call service Error steri run Error in Steri Run routine Reset unit If the error occurs repeatedly call service X X X Power down during steri run Power failure during Steri Run routine Restar...

Page 125: ...ient temperature call field service X X X Actual value low Actual value Set value 1 C 3 4 If the error does not reset automatically call field service X X X Actual value not plausible Implausible temperature signal Reset unit If the error occurs repeatedly call service X X X Calibration values too high low Max adjust value for temperature exceeded not attained Acknowledge alarm enter different tar...

Page 126: ...d automatic X X X Calibration values too high low Max adjust value for CO2 exceeded not attained Acknowledge the alarm X Error communication Sensor not communicating with the main board automatic X X X Error Gas cylinder changeover switch Gas cylinder changeover switch not communicating with the main board automatic X X X No gas Both CO2 cylinders are empty Swap at least one CO2 cylinder X X X RH ...

Page 127: ...e gas supply source Replace gas cylinder Increase primary pressure to 1 bar 14 5 psi max Check feeding line X X X Error communication Sensor not communicating with the main board Request service X X X Error Gas cylinder changeover switch Gas cylinder changeover switch not communicating with the main board automatic X X X No gas Both O2 gas cylinders are empty Swap at least one O2 cylinder X X X Cy...

Page 128: ...6 Handling and control Measures after power outage 6 52 HERACELL VIOS 160i 250i Thermo Scientific ...

Page 129: ...cle 4 The water reservoir starts draining Figure 7 1 Drain valve of water reservoir 5 Allow the water reservoir to drain completely into the collecting vessel 6 Remove drain hose valve again 7 Start the Steri Run decontamination routine Launching steri run on page 6 16 Table of Contents Shutting the device down on page 7 1 CAUTION Contamination hazard If the workspace surfaces are contaminated ger...

Page 130: ...een completed 9 Unplug the power connector and protect it against accidental reconnection 10 Close shut off valves of the CO2 O2 N2 supply system 11 Disconnect the gas pressure hoses from the sleeve at the rear of the device 12 Until the device is shut down the workspace must be continuously ventilated Leave the glass door and the outer door open and secure them in this state ...

Page 131: ... disinfection on page 8 3 Steri run decontamination routine on page 8 7 CAUTION Incompatible cleaners Some device components are made of plastic Solvents can dissolve plastics Strong acids or caustic solutions can cause to become brittle of the plastic For cleaning plastic components and surfaces do not use solvents that contain hydrocarbons solvents with an alcohol content of more than 10 or stro...

Page 132: ...ine uses an automated program cycle to decontaminate the complete workspace including the shelf system and the sensors Preparing for disinfection or steri run 1 Remove all samples from the workspace and store them in a safe place 2 Procure a collecting vessel of sufficient capacity 3 Place open end of hose into collecting vessel and lock drain hose valve safely into receptacle 4 The water reservoi...

Page 133: ...s only in adequately ventilated rooms After the disinfectant has been allowed to react wipe the cleaned device components thoroughly dry Observe safety regulations to avoid fire and explosion hazard caused by alcohol containing disinfectants CAUTION Chloride containing disinfectants Chloride containing disinfectants can corrode stainless steel Use only disinfectants that do not affect stainless st...

Page 134: ...e airbox and the HEPA filter The installation and removal of the airbox and the HEPA filter are described in section Replacing the HEPA filter on page 9 7 CAUTION Health hazard The surfaces of the workspace may be contaminated Contact with contaminated cleaning liquids may cause infections Disinfectants may contain harmful substances When cleaning and disinfecting always observe the safety instruc...

Page 135: ... top part the air duct 1 Figure 8 3 towards the front side of the unit then lift out with a downward movement when the keyholes in the front side mounting tabs clear the retaining pins in the workspace ceiling 4 Separate the top part from the rear part of the air duct 2 Figure 8 3 and remove it from the workspace CLICK 1 2 3 3 4 4 7 8 5 6 ...

Page 136: ...sidues and deposits using a solution of tepid water and dishwashing agent 2 Wipe the surfaces clean using a clean cloth and plenty of clear water 3 Remove the cleaning liquid from the water reservoir and wipe all surfaces of the workspace thoroughly dry 4 Wipe accessories thoroughly dry Final disinfection 1 Spray the surfaces of the workspace the shelving system and parts removed in previous steps...

Page 137: ...e 2 Turn on the device using the power switch 3 Activate and start the decontamination routine 4 After a steri run routine power the unit down 5 Remove the airbox 1 Figure 8 2 and re install the HEPA filter 2 Figure 8 2 Note Conditions that prevent the start of the Steri Run decontamination routine The steri run decontamination routine cannot be started if one of the following failure conditions e...

Page 138: ...prox 1 5 h After the decontamination temperature has been created the decontamination phase of approx 90 minutes is started The temperature is maintained at 180 C Cool down phase approx 8 h The device cools down until the originally set temperature set value is reached CAUTION Hot surfaces The handle and the screen of the glass door the interior panel of the outer door as well as the surfaces of t...

Page 139: ...eri run menu Progress 2 steri run menu Exit and stop steri run Press the End key 3 The system returns to the main menu 3 Activating steri run Press the START key The steri run Progress dialog window is displayed 4 To air the workspace open both device doors when the audible alarm sounds after 30 seconds 5 Remove all samples from the workspace 6 Drain water from the reservoir wipe up any residual w...

Page 140: ...upted at any time 1 Cancel steri run Press the Stop key If the Stop key in the status display is depressed the steri run stop dialog box is displayed for a safety scan The routine can now be permanently cancelled or resumed 2 To stop steri run Press the End key 3 The error message is displayed If the error message is confirmed the system returns to the main menu 3 To resume steri run Press the Bac...

Page 141: ...ation routine automatically changes to the cool down phase the audible alarm sounds 1 To confirm the audible alarm Press any position on the display The audible alarm is switched off The End key is displayed If the decontamination routine is not cancelled after this cool down to the set temperature occurs 2 Cancel steri run Press the End key 3 The error message is displayed If the error message is...

Page 142: ...y The decontamination routine must be stopped manually Figure 8 8 To stop steri run To stop steri run Press the End key 3 The system returns to the main menu Note Opening door during contra con decontamination If the door will be opened and closed again whilst contra con decontamination is running the routine returns to a phase which will ensure a continuation without faults Note Risk of burns Do ...

Page 143: ... controller Electrical safety check in accordance with the relevant national regulations e g BGV 3 Table of Contents Inspections and checks on page 9 1 Service Intervals on page 9 2 Preparing Temperature Calibration on page 9 2 Temperature Calibration Procedure on page 9 3 Preparing the CO2 calibration on page 9 5 CO2 calibration procedure on page 9 6 Replacing the HEPA filter on page 9 7 Replacin...

Page 144: ...ure controller of the oven is set to the value measured during the temperature comparison measurement Use a calibrated measuring instrument with an accuracy of 0 1 C for this test To minimize temperature variations during the measurement put the measuring sensor in an isothermal container such as a bowl filled with glycerol before placing it in the workspace Use the center of the work space as the...

Page 145: ...of the workspace Alternatively a temperature sensor may be positioned in this location Route the connecting cable either through the measurement opening in the glass door 3 or through the access port 1 in the rear panel of the device 4 Close the doors 5 Wait until the temperature value displayed on the measuring instrument has stabilized 6 Temperature calibration procedure Temperature Calibration ...

Page 146: ...ration key Figure 9 3 Temperature Calibration Procedure 4 Enter the measured value target value The targeting value can be increased or reduced in increments if you keep the key or the key depressed the function switches to a rapid increase reduction after approx 3 seconds another increase reduction occurs To increase the targeting value Press the key To reduce the set value Press the key 5 Accept...

Page 147: ...surement must be performed when the device is completely heated up Comparison measurement procedure 1 Turn on the device using the power switch 2 Adjust the CO2 set value and wait until the unit has fully heated up and developed the correct humidity level 3 Insert the measuring instrument probe through the measurement opening into the workspace Wait until the CO2 value displayed by the instrument ...

Page 148: ...igure 9 4 Gas tight screen measurement opening CO2 calibration procedure Measurement example CO2 set value 5 Reference value 5 6 1 Press the CO2 display key The CO2 menu is displayed 2 To exit the CO2 menu Press the End key 3 3 To enter the Calibration submenu Press the Calibration key 1 ...

Page 149: ...ss the Save key The system returns to the main menu The CO2 display shows the actual value currently measured in the workspace Replacing the HEPA filter The HEPA filter is mounted under a plastic airbox at the bottom of the rear wall in the workspace Steps for replacing the HEPA filter 1 Power down the unit shut off the gas supply and allow the workspace to vent 2 Take the center and bottom insert...

Page 150: ...ERACELL VIOS 160i 250i Thermo Scientific 3 Lift the water reservoir cover at the front side 1 Figure 9 6 Figure 9 6 Removing the airbox 4 Pull the water reservoir cover out at the front 2 Figure 9 6 5 Remove the airbox with the HEPA filter 2 1 2 2 ...

Page 151: ...ervoir cover panel 10 If the unit has been operated without the HEPA filter previously activate the HEPA filter in the user configuration according to instructions Activating de activating the HEPA filter on page 6 39 11 If necessary set the reminder interval for the HEPA filter replacement in the user configuration according to instructions Reminder interval setting on page 6 26 The reminder inte...

Page 152: ... Figure 9 8 from the threaded hole 3 Figure 9 8 6 When installing the new gas inlet filter make sure that the plastic thread is not canted Install the filter using caution 7 Install the retainer Procedure for gas supply gas inlet filter 8 Connect the gas hose to the gas inlet filter sleeve and secure it using the hose clamp Check to see if the gas hose is securely seated on the sleeve Figure 9 8 G...

Page 153: ...9 Maintenance Replacing the Door Seal Thermo Scientific HERACELL VIOS 160i 250i 9 11 Replacing the Door Seal Note We recommend having the door seal replaced by a service technician or by expert staff ...

Page 154: ...9 Maintenance Replacing the Door Seal 9 12 HERACELL VIOS 160i 250i Thermo Scientific ...

Page 155: ...e decontaminated Clean the unit components thoroughly then disinfect or decontaminate them depending on application Attach a declaration of non objection with details of performed decontamination measures to the items that are to be disposed of Note Recycling Service Thermo Fisher Scientific offer a recycling service for discarded components Component Material Thermal insulation components Glass w...

Page 156: ...shelves Stainless steel 1 4301 Copper Insert for pressure compensation opening Stainless steel 1 4301 base 1 4404 sinter filter Glass screen Sodium silicate glass Sensor block TCD Stainless steel 1 4301 Cables Plastics and silicon sheathed copper stranded wire Elastomers general Silicone Filter HEPA filter micro fine glass Gas filter with polypropylene casing and GF PTFE membrane Pre filter stainl...

Page 157: ...Thermo Scientific HERACELL VIOS 160i 250i 11 1 11 Specifications Content HERACELL VIOS 160i on page 11 2 HERACELL VIOS 250i on page 11 6 ...

Page 158: ...accessories kg 78 4 Thermal Thermal safety features to DIN 12880 2007 05 Class 3 1 Overtemperature controller providing alarming function upon excessive temperature condition Ambient temperature range C 18 34 Ambient temperature for stacked devices C 18 28 Temperature control range incubation operation C RT 3 55 Temperature deviation from set value over time DIN 12880 part 2 at 37 C C 0 1 Temperat...

Page 159: ... standard feature set See calibration instructions for detailed information Constant humidity at 37 C high humidity mode Constant humidity at 37 C low humidity mode rH rH approx 93 approx 90 Others Sound pressure level DIN 45 635 part 1 dB A 50 Relative ambient humidity rH max 80 Altitude of site m above sea level max 2000 Name Unit Value ...

Page 160: ... 8 max 1 11 6 14 5 psi Measurement and control range Vol 1 21 or 5 90 Control deviation over time Vol 0 2 O2 measuring cell Accuracy at 37 C and 21 O2 O2 0 5 option 1 21 O2 2 0 option 5 90 O2 Electrical Rated voltage V 1 N PE 230 V AC 10 1 N PE 220 V AC 10 1 N PE 120 V AC 10 1 N PE 100 V AC 10 Rated frequency Hz 50 60 Degree of protection IEC 60529 IP 20 Protection class I Overvoltage category EN ...

Page 161: ...ntific HERACELL VIOS 160i 250i 11 5 Rated power kW 230 V Decontamination 1 06 Incubation 0 56 220 V Decontamination 0 97 Incubation 0 51 120 V Decontamination 1 01 Incubation 0 55 100 V Decontamination 0 72 Incubation 0 39 EMV class B Name Unit Value ...

Page 162: ... to DIN 12880 2007 05 Class 3 1 Overtemperature controller providing alarming function upon excessive temperature condition Ambient temperature range C 18 34 Ambient temperature for stacked devices C 18 28 Temperature control range C RT 3 55 Temperature deviation from set value over time DIN 12880 part 2 C 0 1 Temperature deviation from set value spatial DIN 12880 part 2 at 37 C 1 C 0 3 Duration o...

Page 163: ... 7 1 determined on the basis of DIN 12880 for devices with standard feature set See calibration instructions for detailed information Others Sound pressure level DIN 45 635 part 1 dB A 50 Relative ambient humidity rH max 80 Altitude of site m above sea level max 2000 Name Unit Value ...

Page 164: ... 8 max 1 11 6 14 5 psi Measurement and control range VoI 1 21 or 5 90 Control deviation over time VoI 0 2 O2 measuring cell Accuracy at 37 C and 21 O2 O2 0 5 option 1 21 O2 2 0 option 5 90 O2 Electrical Rated voltage V 1 N PE 230 V AC 10 1 N PE 220 V AC 10 1 N PE 120 V AC 10 1 N PE 100 V AC 10 Rated frequency Hz 50 60 Degree of protection IEC 60529 IP 20 Protection class I Overvoltage category EN ...

Page 165: ...ntific HERACELL VIOS 160i 250i 11 9 Rated power kW 230 V Decontamination 1 26 Incubation 0 76 220 V Decontamination 1 16 Incubation 0 69 120 V Decontamination 1 25 Incubation 0 75 100 V Decontamination 0 89 Incubation 0 53 EMV class B Name Unit Value ...

Page 166: ...11 Specifications HERACELL VIOS 250i 11 10 HERACELL VIOS 160i 250i Thermo Scientific ...

Page 167: ...cation on page 12 4 Overview of general parameters addresses 0xxx on page 12 5 Overview of incubator parameters addresses 2xxx on page 12 5 Error memory structure on page 12 8 Data logger structure on page 12 11 Examples of data logger codes on page 12 15 Program HERACELL VIOS 160i HERACELL VIOS 250i on page 12 21 Note Installing the USB port with the virtual COM port If the USB port is to be used...

Page 168: ...WIN VISTA Installing the USB port driver Plug the USB cable into the USB interface port option in the control box of the HERACELL VIOS 160i HERACELL VIOS 250i and connect with a PC As soon as the Windows Hardware Detector has identified the USB port the Find New Hardware Wizard dialog box opens 1 Choose the INSTALL FROM A LIST OR SPECIFIC LOCATION ADVANCED option ...

Page 169: ...nstall from a list or specific location Advanced option 3 Select the data CD as source 4 On the data CD select the DRIVER sub directory 5 The installation routine installs the driver EVAL22 Board USB After the installation has been completed successfully the routine is completed with FINISH ...

Page 170: ...tion of protocol Character coding ASCII characters capital letters are not allowed Reading parameters Query aaaa bb cc CR or aaaa bb XXXX cc CR Response aaaa bb XXXXX cc CR where aaaa parameter adress bb amount of payload in this telegram 00 ff cc checksum Inverted XOR of all bytes without checksum and CR XXXX bb bytes user data Description of the response elements aaaa parameter address bb amount...

Page 171: ...er Reading general parameters Overview of incubator parameters addresses 2xxx Incubator parameters are divided into the basic parameters of the three control loops temperature CO2 and O2 the internal function parameters of operating functions and data logging Failure message Description 0 Error in telegram structure or check sum 1 Unknown error or unknown parameter 2 Internal memory error 3 Data e...

Page 172: ...perature CO2 O2 rH ref temp 33 bytes hexadecimal value with the format xxxxxxxx xxxx xxxx xxxx xxxx 2010 Specified actual and reference temperature 2 23 bytes decimal value with the format xxx xx xxx xx xxx xx 2020 Specified and actual CO2 content 2 15 bytes decimal value with the format xxx xx xxx xx 2030 Specified and actual O2 content 2 15 bytes decimal value with the format xxx xx xxx xx 204a ...

Page 173: ... xx xx xx xx xx 2105 Status of run 1 current CO2 offset waiting period minutes seconds auto start Date and time of last start 25 bytes decimal value with the format xx xx x xxx xx xx xx xx xx xx 2140 Status of as cylinder changeover switch Read CO2 3 2 bytes hexadecimal with the format xx 2141 Status of as cylinder changeover switch Read O2 3 2 bytes hexadecimal with the format xx 2300 Read the Er...

Page 174: ...consists of 1 characters Bytes 2 11 consist of 2 characters Therefore a response consists of 1 10 x 2 21 data bytes plus separator A data set always delivers the date the time the faulty control loop the device status and the error message Bit Disinfection auto start 0x00 Steri run not activated auto start not activated 0x01 Initialization Initialization 0x02 Wait until open door time expires Wait...

Page 175: ...has occurred and the set temperature is too high 00 0b 01 06 0f 37 22 8000 0002 Typ fehlerhafter Regelkreis 0x00 Fehler im Regelkreis Temperatur 00 Datum Tag 0x0b der 11 des Monats 0b Datum Monat 0x01 Januar 01 Datum Jahr 0x06 2006 06 Uhrzeit Stunden 0x0f 15 Uhr 0f Uhrzeit Minuten 0x37 55 Minuten 37 Uhrzeit Sekunden 0x22 34 Sekunden 22 Gerätestatus 0x8000 Gerätefehler vor handen 8000 Fehlermeldung...

Page 176: ...t communicating 0x0008 Implausible mainboard parameter EEPROM defective 0x0010 Data logger defective unit can continue to function 0x0020 Error for disinfection steri run 0x0040 Power down during steri run 0x0080 Error auto start 0x0100 ADC test failed 0x0400 Fan error 0x1000 IR sensor system replaced info 0x2000 auto start active info 0x4000 Disinfection active info 0x8000 Device error has occurr...

Page 177: ...he oldest entry and output the first data sets Query 2401 00 cc CR Output of the following data sets the reading pointer automatically moves gradually from the older entries to the current entries Query 2402 00 cc CR 0x0004 Actual value low 0x0010 Calibration values too high low 0x0020 Error communication to sensor 0x0040 Error communication to gas cylinder changing unit 0x0080 No gas available bo...

Page 178: ...acters 0x32 2 and 0x33 3 Therefore a response consists of up to 7 X 16 112 bytes i e 224 ASCII characters The date and time without seconds the device status and the type of data logger entry are always transmitted in a data set byte 0 7 and ASCII characters 0 15 Also depending on the entry the current actual values or set values of the control loops or other parameters can be entered byte 8 15 or...

Page 179: ... 0000 0177 2 0028 00d4 0000 Type of data logger entry 0x01 Standard entry which is saved every 60 s Date Day 0x0b 11th of months Date Months 0x01 January Date Year 0x06 2006 Time Hours 0x0f 15 o clock Time Minutes 0x37 55 minutes Device status 0x0000 everything ok Based on type here the current temperature in 1 10 Celsius 0x0177 37 5 C Based on type here the current CO concentration in 1 10 0x0028...

Page 180: ...erature CO2 O2 and rH 0x21 New error CO2 Status error registration for temperature CO2 O2 and rH 0x22 New error O2 Status error registration for temperature CO2 O2 and rH 0x2F New error system Status error registration for temperature CO2 O2 and rH 0x30 Power reset Set value for temperature CO2 O2 and rH 0x31 Door open Current actual values for temperature CO2 O2 and rH 0x32 Door closed Current ac...

Page 181: ...ure CO2 O2 and rH 0x60 Start steri run Status error registration for temperature CO2 O2 and rH 0x61 steri run successfully completed Current actual values for temperature CO2 O2 and rH 0x62 steri run completed with error Status error registration for temperature CO2 O2 and rH 0x63 steri run stopped manually Status error registration for temperature CO2 O2 and rH 0x70 Gas mon cylinder A empty Gas m...

Page 182: ... num_2_string uses hexadecimal values to create ASCII characters to be entered into a file read_datalogger edits the received data and writes them into a file Example of a code for a a data logger query char ahex char a char ahex char a char i char hexa 16 0123456789abcdef for i 0 i 16 i if a hexa i return i return 0 send_telegramm void send_telegramm char p char string 15 unsigned char bcc 0xFF c...

Page 183: ...reading_count 1 until reception of CR while p reading_count r return number of received characters return reading_count time_2_str char time_2_str int z char b char i output two numerals for i 1 i 0 i calculate value b i z 10 0x30 reduce default value z z 10 return 2 num_2_string char num_2_str int z char b number with one decimal place char a 12 char i l int rest 0 l 0 Negative number if z 0 set ...

Page 184: ...gger int read_datalogger define SIZE_DATA2 16 define EVENT_STATUS 0x01 unsigned char buffer 300 string 300 unsigned char zahlenstring 150 datestring timestring unsigned char excelstring 150 unsigned char len h i unsigned int read_count status define EVENT_DATA END 0xFF char data int GetTele 0 GetError 0 writing the title line in the file WriteFile FileHandle Date Time Comment Temp Act CO2 Act O2 A...

Page 185: ...e into file len time_2_str numeric string 1 i SIZE_DATA2 excelstring len excelstring len len time_2_str numeric string 2 i SIZE_DATA2 excelstring len excelstring len len time_2_str numeric string 3 i SIZE_DATA2 excelstring len excelstring len len time_2_str numeric string 4 i SIZE_DATA2 excelstring len excelstring len len time_2_str numeric string 5 i SIZE_DATA2 excelstring len excelstring len len...

Page 186: ...E_DATA2 0x100 numeric string 9 i SIZE_DATA2 excelstring len excelstring len len num_2_str numeric string 10 i SIZE_DATA2 0x100 numeric string 11 i SIZE_DATA2 excelstring len excelstring len len num_2_str numeric string 12 i SIZE_DATA2 0x100 numeric string 13 i SIZE_DATA2 excelstring len excelstring len len num_2_str numeric string 14 i SIZE_DATA2 0x100 numeric string 15 i SIZE_DATA2 excelstring le...

Page 187: ...SollO2 numeric string 12 i SIZE_DATA2 0x100 numeric string 13 i SIZE_DATA2 SollrH numeric string 14 i SIZE_DATA2 0x100 numeric string 15 i SIZE_DATA2 WriteFile FileHandle excelstring len WriteFile FileHandle Power on n 10 break case query all events here see Overview of possible event entries in bit coding Overview of event entries Part I on page 12 14 cancel 0xFF indicates the end of the data log...

Page 188: ...e information of the service file is very useful for systematic troubleshooting The data sets are stored in the proprietary format SRF Installing the HERACELL VIOS 160i HERACELL VIOS 250i 1 Starting the installation routine On the data CD in the subdirectory PROGRAMS double click on the file SETUP EXE to open this file 2 Locate the installation directory for the program 3 In the sequence of the gi...

Page 189: ...SION Switch for exiting the program QUIT GENERAL with the submenus PRESETTING for setting the transmission speed and for selecting the serial port TEST COM for testing the communication connection between PC and incubator DATE TIME for adjusting date and time to the desired time zone ERROR LOGGER for reading the error messages DATA LOGGER for reading the event entries SERVICEFILE for reading error...

Page 190: ...Select a transmission speed within the range of 9 600 to 115 200 baud 2 Select the serial port of the PC If the USB driver is installed the virtual Com Port assigned to the USB port can be selected see USB interface on page 12 1 3 Accept the settings Press the CHANGE key 4 Save the settings in an INI file Press the SAVE TO FILE key Note Transmission speeds The transmission speed settings in the us...

Page 191: ...ample of a query for the currently measurable temperature values of the incubator Query default not changeable Address 2010 Address temperature value set value actual value reference value 2 Send the query to the incubator Press the SEND key If the incubator returns a response string the communication connection to the incubator has been established If a connection cannot be established an error d...

Page 192: ...ext boxes must be entered in the format DD MM YY day month year 2 Accept the input data Press the SET key ERROR LOGGER The submenu ERROR LOGGER is used for reading the error messages into the text box of the user surface The data sets can be stored in the metaformat CSV Saving data sets as files Press the SAVE TO FILE key DATA LOGGER The submenu DATA LOGGER is used for reading the event entries in...

Page 193: ... The submenu SERVICEFILE is used for reading error informations and for creating a service file from it saved with the proprietary extension srf The service file is transmitted to the Technical Service of Thermo Fisher Scientific for fault analysis 1 Create the service file Press the CREATE SERVICEFILE key Define file name and saving directory in the Windows dialog box 2 Start saving process Press...

Page 194: ...a communication Program HERACELL VIOS 160i HERACELL VIOS 250i 12 28 HERACELL VIOS 160i 250i Thermo Scientific PASSWORD The submenu PASSWORD can only be accessed by the service personnel of Thermo Fisher Scientific ...

Page 195: ...mo Scientific HERACELL VIOS 160i 250i 13 1 13 Device log Please list carried out works here Device type Part No Serial number Service number Location Operator s note Work carried out Comments Date Signature ...

Page 196: ...13 Device log 13 2 HERACELL VIOS 160i 250i Thermo Scientific ...

Page 197: ...desk email service lpg germany de ThermoFisher com Enquiries from Europe Middle East and Africa Phone 49 0 6184 90 6940 Fax 49 0 6184 90 7474 Email info labequipment de thermofisher com Postal address USA Thermo Scientific 275 Aiken Road Asheville NC 28804 USA Enquiries from North America Phone 1 800 879 7767 1 800 879 7767 Fax 1 828 658 0363 Emai linfo labequipment thermofisher com Enquiries from...

Page 198: ...505118 or 86 10 8419 3588 India Sales 91 22 6716 2200 Service Toll free 1 800 22 8374 or 91 22 6716 2200 Japan Sales 81 45 453 9220 Service 81 45 453 9224 Enquiries from the Rest of Asia Australia New Zealand Sales 852 2885 4613 Service 65 6872 9720 Enquiries from Countries not listed Rest of EMEA Sales 49 6184 90 6940 or 33 2 2803 2000 Service 49 6184 90 6940 Enquiries from Europe Austria Sales 4...

Page 199: ... 250 Netherlands Sales 31 76 579 5555 Service 31 76 579 5639 Russia CIS Sales 7 812 703 4215 Service 7 812 703 4215 Spain Portugal Sales 34 93 223 0918 Service 34 93 223 0918 Switzerland Sales 41 61 716 7755 Service 41 61 716 7755 UK Ireland Service 44 870 609 9203 Sales 44 870 609 9203 2013 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientifi...

Page 200: ...l rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Thermo Fisher Scientific 81 Wyman Street Waltham MA 02451 Thermo Fisher Scientific San Jose CA USA is ISO Certified ...

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