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Thermo Scientific

Evolution One Series Spectrophotometer User Guide

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Evolution One Series Spectrophotometers

The Evolution™ One series UV-Visible spectrophotometers includes two models:

• Evolution One

• Evolution One Plus

Safety Considerations

Each person using the spectrophotometer should read the general safety information in the
Site and Safety Information guide (see the documentation CD) and the instrument-specific
safety information provided here.

Contents

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Safety Considerations

•

Operating Precautions

•

Spectrophotometer Basics

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Installing and Removing Accessories

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Using the Spectrophotometer

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Contents

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Safety Label Locations

•

Lifting or Moving the Instrument

WARNING

Do not operate this system without following the safety precautions described

in this manual and the documentation that came with your system.

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Summary of Contents for Evolution One Series

Page 1: ...Evolution Series UV Visible Instruments Evolution One Series Spectrophotometer User Guide 269 350500 Revision A October 2021 ...

Page 2: ...nly System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in t...

Page 3: ... for Items Covered by Warranty 6 Update Policy 6 Trademarks 7 Site Preparation and Safety 7 Chapter 2 Evolution One Series Spectrophotometers 1 Safety Considerations 1 Safety Label Locations 2 Lifting or Moving the Instrument 3 Operating Precautions 4 Spectrophotometer Basics 4 Spectrophotometer Components 5 Connectors 6 Removable Panels 8 Corrosion Protection 13 Installing and Removing Accessorie...

Page 4: ...ectrophotometer User Guide Thermo Scientific Using the Spectrophotometer 23 Computer Control Instruments 23 Maintenance 24 Routine Maintenance 24 Cleaning the Instrument 25 Removing and Installing the Detector Module 25 Ordering Parts 27 ...

Page 5: ...Contents Thermo Scientific Evolution One Series Spectrophotometer User Guide v ...

Page 6: ......

Page 7: ...e series UV Visible spectrophotometers and accessories About the Hardware Contents Overview Setting Up the Instrument Before Using the Spectrophotometer About This Document Contacting Us E mailing Data Files Product Warranty Trademarks Site Preparation and Safety Contents Overview Setting Up the Instrument Before Using the Spectrophotometer ...

Page 8: ...f wavelengths Quant to set up and perform quantitative analyses of sample data Kinetics to make time and temperature based kinetics measurements Live Display for quick measurements and simplified data collections in Fixed or Scan mode Setting Up the Instrument The spectrophotometer can be run from a Windows compatible computer connected to the instrument Computer Control Computer Control instrumen...

Page 9: ...y Evolution One Series Spectrophotometers Complete instructions for using and maintaining the instrument Accessories Complete instructions for using accessories with the instrument WARNING Indicates a hazardous situation which if not avoided could result in death or serious injury CAUTION Indicates a hazardous situation which if not avoided could result in minor or moderate injury NOTICE Follow in...

Page 10: ...rized dealers Thermo Fisher Scientific warrants that this product is free from defects in labor and materials and shall conform to its product specifications as defined in the product user documentation This warranty covers parts except those specified below and labor and applies only to equipment which has been installed and operated in accordance with the documentation supplied by Thermo Fisher ...

Page 11: ...diately upon receipt and any problems reported to Thermo Fisher Scientific within the above specified warranty period The following warranties apply to instrument light sources 1 Xenon lamps are warranted against failure for a period of three 3 years 2 Tungsten and deuterium lamps will be replaced under warranty if they fail on the initial power up or within the first month of operation Thermo Fis...

Page 12: ...mation A Return Authorization Number must be obtained from Thermo Fisher Scientific Technical Service before returning any product for in warranty repair or replacement Shipping Cost for Items Covered by Warranty It is customer s responsibility to pay for the shipment of the product under warranty to Thermo Fisher Scientific This warranty covers return shipment standard ground charges for returnin...

Page 13: ...Pont or its affiliates in the United States and or other countries Starna is either a trademark or a registered trademark of Starna Scientific Limited in the United States and or other countries Hellma is either a trademark or a registered trademark of Hellma GmbH in the United States and or other countries AccuVac and Hach are either trademarks or registered trademarks of Hach Company in the Unit...

Page 14: ...1 Introduction Site Preparation and Safety 8 Evolution One Series Spectrophotometer User Guide Thermo Scientific ...

Page 15: ...rmation in the Site and Safety Information guide see the documentation CD and the instrument specific safety information provided here Contents Safety Considerations Operating Precautions Spectrophotometer Basics Installing and Removing Accessories Using the Spectrophotometer Maintenance Ordering Parts Contents Safety Label Locations Lifting or Moving the Instrument WARNING Do not operate this sys...

Page 16: ... Safety Label Locations This section shows the locations of safety labels on the spectrophotometer If there are questions or problems with the safety labels contact us using the information provided at the beginning of this document Back view Right side view Compliance label UV radiation warning label under mercury lamp access cover ...

Page 17: ...otometer User Guide 3 Left side view Related Topics Removing and Installing the Detector Module Lifting or Moving the Instrument To avoid risk of injury use proper techniques when lifting or moving the instrument or other system components UV radiation warning label under detector access cover Hand hold Hand hold ...

Page 18: ...recise optical components Handle it carefully and follow these precautions Do not allow moisture to leak into the instrument interior Wipe off spilled chemicals immediately Do not drop the instrument Protect the instrument from mechanical shock Protect the instrument from dust Spectrophotometer Basics Contents Spectrophotometer Components Connectors Removable Panels Corrosion Protection ...

Page 19: ...stance from the bottom of the cell to the center of the light beam for the spectrophotometer is 8 5 mm Related Topics Opening and Closing the Sample and Reference Compartment Doors Computer Control Insight Pro Software Opening and Closing the Sample and Reference Compartment Doors To open the sample compartment press the button on its door To close the compartment slide the door forward until it l...

Page 20: ...nected to an external computer with the proper software installed Insight Pro Software All instruments include our Insight Pro software for data collection and analysis See the Insight Pro Software User Guide Connectors This section shows the locations of the connectors inside and outside the spectrophotometer Outside Cover This illustration shows the connectors on the instrument outside cover Bac...

Page 21: ...Sample Compartment Trigger Connects to optional accessories that accommodate external trigger input or output For more information find the Integrated Fiber Optic Module User Guide in the documentation media Insight Pro can be used to send a 3 3V TTL output signal that triggers an installed accessory to begin an operation The signal can be sent at the start or end of a measurement See Settings Pre...

Page 22: ...lustration identifies the connectors inside the sample compartment Related Topics Installing a Smart Accessory Removable Panels This section covers panels that can be removed to allow access to features on the instrument Smart Accessory connector Contents Sample Compartment Front Panel Tubing Access Panel Mercury Lamp Access Cover Detector Access Cover Detector Access Panel ...

Page 23: ... To remove the front panel open the sample compartment door and then pull the panel forward and up to free it To replace the panel align the tab on the bottom edge with the slot in the instrument cover and press the panel in place Related Topics Installing a Manual Accessory Installing a Liquid Thermostatted Accessory Installing a Smart Accessory Removable panel Tab ...

Page 24: ... The connectors fit 1 4 inch internal diameter tubing An adapter may be required to connect the tubing to the accessory or recirculator Purchase adapters separately Cords and cables for installed accessories can also be routed behind this panel Push the cord through the foam and the opening below it The panel itself can be removed temporarily to accommodate a large connector To remove the panel lo...

Page 25: ...ccess Cover Some accessories require removing the instrument detector module The module is beneath the detector access cover shown below Avoid UV radiation hazard in the detector compartment Protect eyes and skin from exposure WARNING Avoid UV radiation hazard in the mercury lamp compartment Protect eyes and skin from exposure Mercury lamp access cover CAUTION Safety glasses with side shields or g...

Page 26: ...ermo Scientific Related Topics Removing and Installing the Detector Module Detector Access Panel Detector Access Panel To remove the panel between the detector and sample compartment remove the sample compartment window and then loosen the thumbscrew Detector module cover removed Thumbscrew Window optional Removable panel ...

Page 27: ...rophotometer contains precise optical components that may be damaged by a corrosive environment Before using the instrument to analyze volatile corrosive or caustic samples install the optional sample compartment windows and if necessary purge the sample compartment Related Topics Installing Sample Compartment Windows Purging the Sample Compartment Installing Sample Compartment Windows The optiona...

Page 28: ...lling Sample Compartment Windows Selecting a Purge Gas Installing Purge Selecting a Purge Gas Use dry air or dry nitrogen to reduce or eliminate condensation The purge gas must be free of moisture oil and other reactive materials To remove particulate matter and oil install a 10 micrometer filter Related Topics Installing Sample Compartment Windows Installing Purge Installing Purge Use 1 4 inch in...

Page 29: ...ference Cell Holder 10 mm Rectangular Sample Cell Holder This single cell holder is mounted on the standard baseplate and accommodates standard 10 mm pathlength cells See Installing a Manual Accessory Use the cell lifter to raise the cell for easy removal This cell holder allows fine adjustment of the vertical and horizontal position of the cell This feature is useful for aligning reduced volume a...

Page 30: ... holder in the reference position See Installing a Reference Cell Holder The standard reference holder accommodates rectangular reference cells with these pathlengths 10 mm 20 mm 40 mm 50 mm and 100 mm Related Topics 10 mm Rectangular Sample Cell Holder Installing a Manual Accessory Manual sampling accessories are mounted on a common baseplate The kinematic mount ensures correct alignment of the a...

Page 31: ...wo alignment holes on its baseplate fit over pins A and B in the sample compartment 4 Gently push down on the front edge of the baseplate until it snaps in place under the toggle 5 Close the sample compartment door See Opening and Closing the Sample and Reference Compartment Doors For operating instructions and installation details see the user guide for the accessory To remove a manual accessory ...

Page 32: ...s ensure software methods are properly configured These accessories install in the sample compartment A connector under the accessory baseplate provides data communication and power It is not necessary to power off the instrument while installing or removing a Smart Accessory Sample compartment top view To install a Smart Accessory 1 Open the sample compartment door See Opening and Closing the Sam...

Page 33: ...ress down on the front of the accessory to secure the connection The software displays a prompt to initialize the accessory 5 Replace the sample compartment front panel See Sample Compartment Front Panel 6 Close the sample compartment door See Opening and Closing the Sample and Reference Compartment Doors 7 Click OK to initialize the accessory Initialization reads information about the accessory a...

Page 34: ...cs Installing a Liquid Thermostatted Accessory Installing a Manual Accessory Installing a Reference Cell Holder Installing a Liquid Thermostatted Accessory A liquid thermostatted accessory has ports and internal chambers for circulating fluids through the unit for temperature controlled measurements Other accessories circulate fluids to cool heated components The Recirculator Tubing Kit provides p...

Page 35: ... use a tubing adapter 4 Use additional tubing and adapters not included if necessary to connect the internal chambers in the sample compartment to the ports on the accessory For a cell changer set the changer to position 1 before connecting the tubing to the ports on the accessory Use just enough tubing to allow the cell changer to move through all possible positions This requires approximately 10...

Page 36: ...er See Opening and Closing the Sample and Reference Compartment Doors 2 Grasp the cell holder by the handle and lower it into the reference compartment 3 Insert the pin in the alignment hole and press the holder in place 4 Close the reference compartment cover For descriptions of the available reference holders see Reference Cell Holders or search for that name in the Insight Pro Help system Note ...

Page 37: ...Related Topics Setting up a Computer Control Instrument Turning off a Computer Control Instrument Maintenance Setting up a Computer Control Instrument To set up a Computer Control instrument 1 Connect the supplied USB cable to the Computer port on the back of the spectrophotometer 2 Connect the other end of the cable to the USB port on the back of the computer 3 Turn on the spectrophotometer main ...

Page 38: ...Pro software 2 If necessary turn off the spectrophotometer main power switch on the back panel Related Topics Setting up a Computer Control Instrument Maintenance Maintenance Routine Maintenance Clean the instrument regularly using approved methods wipe off spilled chemicals immediately Related Topics Maintenance Tip For routine use leave the power switch on the instrument back panel in the On pos...

Page 39: ...moving and Installing the Detector Module Some accessories have a dedicated detector and require removing the instrument detector module before they can be installed Avoid UV radiation hazard in the detector compartment Protect eyes and skin from exposure To remove the detector module 1 Turn off the spectrophotometer main power switch on the back panel 2 Remove the detector access cover See Detect...

Page 40: ...ter See Using the Spectrophotometer 2 Remove the detector access cover See Detector Access Cover 3 Unplug the accessory detector cable if necessary 4 Position the detector module so its thumbscrew faces left 5 Line up the connector on the bottom of the detector module with the connector in the detector compartment and then press down on the module to secure the connection 6 Tighten the thumbscrew ...

Page 41: ...Thermo Scientific Evolution One Series Spectrophotometer User Guide 27 Related Topics Maintenance Detector Access Cover Detector Access Panel Ordering Parts To order replacement parts contact us using the information provided at the beginning of this document ...

Page 42: ...2 Evolution One Series Spectrophotometers Ordering Parts 28 Evolution One Series Spectrophotometer User Guide Thermo Scientific ...

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