Thermo Scientific CryoStar NX50 Operator'S Manual Download Page 46

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Setting Preferences

Quick Freezing of the Specimen

The cryobar provides on-demand, quick freezing of specimens.

Notes

When the cryobar is activated, it will take approximately 2 - 3 minutes for the cryobar to reach a temperature of -60°C.

The cryobar remains active for approximately 10 minutes.

The specimen is frozen onto the specimen chuck using a freezing compound and the cryobar.

Note

To ensure optimal adhesion of the specimen to the chuck, store the chucks at room temperature prior to use.



Press the "Cryobar" button on the Main screen to activate it.



The "Cryobar" button is highlighted in blue when the function is activated.



To turn off the Cryobar function, press the highlighted "Cryobar" button again.

Summary of Contents for CryoStar NX50

Page 1: ...Thermo Scientific CryoStar NX70 NX50 Operator Guide 387928 ...

Page 2: ...ientific representative This Operation Manual may not in whole or in part be copied photocopied reproduced translated or converted to any electronic or other form without prior written consent of Thermo Fisher Scientific All information contained in this manual is proprietary and confidential and the exclusive property of Thermo Fisher Scientific This Thermo Fisher Scientific manual is protected b...

Page 3: ...the instructions contained in this manual are not specific to any one variant Thus some features described may not be available on your instrument However in cases where information only pertains to either the NX70 or NX50 models this will be indicated by the presence of one of the following symbols Information only applies to CryoStar NX70 instruments Information only applies to CryoStar NX50 ins...

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Page 5: ...es that a surface is cold If this symbol appears on the instrument always refer to the operator guide Take suitable precautions This symbol is show in the manual and on the instrument to indicate that hand injuries are possible due to moving parts Take suitable precautions This symbol is shown in the manual to indicate the risk of injury caused by sharp microtome blades and knifes Take suitable pr...

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Page 7: ...agnetic Blade Tool 21 Blade Positioning and Orientation 22 Adjusting the Cutting Angle 23 Adjusting the Anti Roll Plate 25 Specimen Setup 26 Specimen Orientation NX70 26 Specimen Orientation NX50 28 Filling with Disinfectant to Perform the Cold D Function 31 Filling the Cryostat with Disinfectant Solution Sanosil 31 4 Operation 33 Switching On 33 System Interfacing 34 Using the Touchscreen 34 Main...

Page 8: ...ge 67 Time and Date 68 Start Time 72 Defrost 75 Immediate Defrosting 75 Programming the Daily Defrost Cycle 78 Screen 79 Retraction 81 Data Log 82 Default Settings 83 Service 85 Emergency Stop 86 Standard Emergency Stop Function 86 Optional Foot Pedal Emergency Stop 86 Cooling Processes 87 Specimen and blade holder cooling 87 Sectioning Procedures 88 Cutting Movement and Retraction 88 Trimming and...

Page 9: ...s for Sectioning Different Types of Fresh Specimen 102 6 Cleaning and Maintenance 103 Cleaning the Touch Screen Display 103 Shutting Off for Cleaning 103 Cleaning and care of the Blade Holder 103 Emptying the Defrosting and Cold D Liquids 104 Exchange the Vacutome Filter Bag 105 Adjustment of the Movable Specimen Head Jaw 107 Transportation of the Instrument 108 Appendices 110 Appendix A Spares an...

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Page 11: ... many of the reagents used with this instrument may be flammable Do not introduce any source of ignition into or near the instrument once it has been loaded with reagents Do not remove any panels or covers The instrument does not have any user serviceable parts The instrument must be properly connected to a good earth ground via the Mains input supply Position the instrument such that it is possib...

Page 12: ...t at the customers own risk All the chemicals recommended by Thermo Fisher Scientific have auto ignition temperatures considerably above any surface temperature that can be reached during a single fault failure on the instrument The instrument contains no source of ignition in any areas of the instrument where chemicals are stored or are likely to leak into in a single fault condition The operator...

Page 13: ...mo com WEEERoHS Warranty Statement We at Thermo Fisher Scientific are proud of our quality reliability and of our after sales service We continuously strive to improve our service to our customers Please ask you distributor or Thermo Fisher Scientific representative about Service Contracts which can keep your purchase in peak condition for many years to come Warranty provisions necessarily vary to...

Page 14: ...4 Product Return Safety Declaration ...

Page 15: ...Any other use of this instrument is considered as improper operation Instrument Features The CryoStar NX70 NX50 are precision engineered ergonomically designed high performance cryostats in modular design with a stainless steel cooling chamber Some of its features are Knife Blade Holder cooling Specimen cooling Ergonomic height adjustment with integrated ergonomic casing Colour touch screen user i...

Page 16: ...Operating Limits 5 C to 35 C 41 F to 95 F Temperature Recommended Operation 15 C to 30 C 59 F to 86 F Note Performance may deteriorate when operated outside of this range Temperature Transport and Storage 20 C to 40 C 4 F to 122 F 70 C 158 F for short exposure Relative Humidity Max 80 RH up to 31 C Decreasing linearly to 50 RH at 40 C Altitude Up to 2000 m 6 500 ft Pollution Degree 2 Over Voltage ...

Page 17: ...m steps Trimming Thickness Range 5 500 μm Trimming Thickness Resolution 5 10 μm in 5 μm steps 10 μm 100 μm in 10 μm steps 100 μm 200 μm in 20 μm steps 200 μm 500 μm in 50 μm steps Specimen Retraction Return Travel 20 μm Vertical Specimen Stroke 64 mm Horizontal Specimen Movement 48 mm Specimen Approach Manual variable speed by joystick control Chuck Size 30 40 mm additional special sizes are avail...

Page 18: ...emperature 15 C Specimen Temperature 15 C Cutting Window 0 mm OFF Favorites Program 1 Program 2 Program 3 15 C 15 C 20 C 20 C 25 C 25 C Cryobar OFF Vacutome OFF Disinfection OFF Disinfection Cycle Automatic ON 03 00 Night Defrost Cycle Automatic ON 00 00 Night Standby Mode 1h After 1 hour the Cryostat enters the Standby Mode automatically Kept temperature 15 C Sleep Mode 3h After 3 hours the Cryos...

Page 19: ...rument are encased in cardboard and cushioned with foam spacers inside the container A wooden cover protects the top of the instrument In unpacking be aware of the weight 200 Kg 440 lbs of the instrument Safe handling of the installation requires the involvement of two people Handling Place the crate on a flat surface while ensuring that there are no objects underneath the pallet Remove the wooden...

Page 20: ...pallet stand BEHIND NOT in front of the instrument Carefully roll the instrument off the pallet and on to the floor Move the instrument to the desired location while leaving space on the left and right hand Keep all packing and transportation materials together in case of future need In the future if the instrument is transported by forklift truck train ship or aircraft it must be packed in the or...

Page 21: ...t be accessible at all times The location must be free from draughts open doors or air conditioning systems The location must not expose the cryochamber to direct sunlight Enough room for height adjustment Note These measures are intended to reduce the build up of frost and maintain consistent cooling temperatures resulting in more favorable working conditions Locations which have high humidity an...

Page 22: ...ation of Parts Overview 1 Cryochamber 6 Connections Panel 2 Cryochamber Window 7 Handwheel 3 Touch Screen 8 Handwheel Mechanical Brake 4 Cold D Reservoir Cover 9 Water Drain Reservoir Cover 5 Emergency Stop Button ...

Page 23: ...3 Instrument Setup 13 Connections Panel 10 Power Switch 13 X1 RJ45 port for Service purposes only 11 Power Socket 14 X2 USB port 12 Rating and Serial Number Label 15 X3 Foot Pedal Connector ...

Page 24: ...7 Trim Button 24 Motorized Cutting Start Stop Button 18 Joystick 25 Handwheel Electronic Brake Button 19 Specimen Head 26 Vacutome Filter Cover 20 Blade Holder with Anti Roll Plate 27 Vacutome Filter Cover Release Knob 21 Vacutome 28 Section Waste Tray 22 Mode Selection Button ...

Page 25: ...trument Setup 15 Blade Holder 29 Lateral Adjustment Lever 33 Blade Holder Base 30 Anti Roll Plate 34 Anti Roll Plate Adjustment 31 Blade Clamping Lever 35 Clearance Angle Adjusting Screw 32 Blade Holder Base ...

Page 26: ...men Head NX70 36 Y axis Fine Adjustment Knob 39 Specimen Head Clamping Lever 37 Specimen Chuck Release Lever 40 Upper Specimen Chuck Jaw Static 38 X Axis Fine Adjustment Knob 41 Lower Specimen Chuck Jaw Moving ...

Page 27: ...3 Instrument Setup 17 Specimen Head NX50 42 Specimen Chuck Release Lever 45 Upper Specimen Chuck Jaw Static 43 Orientation Handle 46 Lower Specimen Chuck Jaw Moving 44 Specimen Head Clamping Lever ...

Page 28: ...should be kept at room temperature The heated sliding window should be closed during periods of inactivity and while preparing specimens to avoid warm air getting into the chamber This will result in more favorable working conditions and a lower frost build up Ensure that the vacutome filter is properly inserted in the filter unit and is suitably maintained see Cleaning and Maintenance Fitting or ...

Page 29: ...ADE WITH THE KNIFE BLADE GUARD AND ACTIVATE THE BRAKES PRIOR TO MAKE MODIFICATIONS TO THE BLADE KNIFE AND SPECIMEN OR CHANGING THE SPECIMEN The blade holder should be fitted with low profile blades 80mm x 8mm 35 facet angle as standard To fit or exchange low profile blades Move the anti roll plate to the side Pull the blade clamping lever towards the front of the instrument to release the clamping...

Page 30: ...20 Carefully slide a low profile blade into the slot Push the blade clamping lever towards the back of the instrument to clamp the blade in position ...

Page 31: ...der in a save way Remove the used blade with the magnetic blade tool Move the anti roll plate to the side Pull the blade clamping lever towards the front of the instrument to release the clamping plate Move the magnetic blade tool over the blade Carefully lift the blade out of the blade holder and dispose of it in accordance with laboratory regulations ...

Page 32: ...of the blade is no longer usable the upper part of the blade holder can be moved without removing the blade To move the upper part of the blade holder Loosen the lateral adjustment lever The upper part of the blade holder should now be free to move left and right Move it either to the left or to the right until the desired area of the blade is directly below the specimen head ...

Page 33: ... cutting angle can be adjusted the recommended cutting angle is approximately 10 for Thermo Scientific low profile blades Note The factory preset cutting angle is 10 this value is the recommended cutting angle for Thermo blades The minimum angle is 8 the maximum angle is 12 Loosen the clearance angle adjustment screw on the lower left site of the blade holder ...

Page 34: ...E HOLDER BASE MIGHT BE COLD USE GLOVES IF NECESSARY Note The clearance angle can be read on the scale on the left side on the upper part of the blade holder Note 10 is indicated by the longer increment in the middle of the scale Re tighten the clearance angle adjusting screw to fix the selected clearance angle ...

Page 35: ...f the anti roll plate is carried out using the knurled screw To raise the height of the Anti Roll Plate turn the screw anti clockwise To lower the height of the Anti Roll Plate turn the screw clockwise Note Always adjust the height of the Anti Roll Plate in small increments ...

Page 36: ...cimen head To clamp and orient the specimen Loosen the specimen chuck release lever and insert the specimen chuck into the jaws of the specimen head Release the specimen chuck release lever again to allow the jaws to grip the chuck Loosen the specimen head clamping lever to allow adjustment of the specimen parallel to the cutting edge ...

Page 37: ...g lever again For further specimen orientation the specimen chuck can also be rotated by 360 as described below Loosen the specimen head clamping lever slightly Rotate the chuck as required Move the specimen head clamping lever back to its original position After the specimen is adjusted set the cutting window limits see Setting the Cutting Window Use the motorized coarse feed to position the blad...

Page 38: ...ch WARNING TO AVOID SEVERE DAMAGE ENSURE THAT THE BLADE DOES NOT CRASH INTO THE SPECIMEN Specimen Orientation NX50 Before sectioning can begin the specimen must be correctly clamped and oriented in the specimen head To clamp and orient the specimen Loosen the specimen chuck release lever and insert the specimen chuck into the jaws of the specimen head ...

Page 39: ...s to grip the chuck Loosen the specimen head clamping lever to allow adjustment of the specimen parallel to the cutting edge Use the orientation handle to orient the specimen on the x and y axes Note The orientation handle will click into place when the center position has been reached ...

Page 40: ... clamping lever back to its original position After the specimen is adjusted set the cutting window limits see Setting the Cutting Window Use the motorized coarse feed to position the blade close to the specimen Tilt the joystick forwards to move the blade holder towards the specimen Note Tilting the joystick further forwards will increase the speed of approach WARNING TO AVOID SEVERE DAMAGE ENSUR...

Page 41: ...MES PER DAY PLUS ONE CYCLE OVERNIGHT Filling the Cryostat with Disinfectant Solution Sanosil To load the cryostat with disinfectant Open the Cold D reservoir cover to access the filling inlet of the disinfectant tank Notes Before filling the tank check the shelf life and the hazardous substance warnings on the disinfectant Always follow the manufacturer s instructions for handling storage and disp...

Page 42: ...oir cover Notes The maximum capacity of the disinfectant tank is 1 liter which is sufficient for approximately 18 Cold D cycles If the fluid level in the disinfectant tank is too low an error message will be shown on the touch screen display Dispose of the empty disinfectant bottle in accordance with laboratory regulations ...

Page 43: ... needs high surge currents when started Do not use multi socket power outlets with small wire sizes for the supply of the instrument Insert the supplied power cord into the power socket and then connect the power cord to the mains supply Install the blade holder if necessary Close the heated window Turn on the power switch Notes The default setting of the blade holder and specimen head temperature...

Page 44: ...SCREEN Main Screen The Main screen appears after the instrument has been switched on 1 Disinfection Cold D Function 9 Favorite Temperatures 2 Cryobar 10 Vacutome Settings 3 Menu 11 Height Adjustment 4 Trimming Thickness 12 Handwheel Brake On Off 5 Blade Temperature 13 Multi Function Button 6 Specimen Head Temperature 14 Cutting Mode 7 Fine Section Thickness 15 Time or Date Service Message Wrench s...

Page 45: ...without saving any changes Arrow keys The Arrow keys are used in several menus to increase or decrease values Use the arrow keys to adjust the value to the required setting 1 Decreases the selected value by multiple steps 3 Increases the selected value by 1 step 2 Decreases the selected value by 1 step 4 Increases the selected value by multiple steps Note The number of steps by which the multiple ...

Page 46: ...emains active for approximately 10 minutes The specimen is frozen onto the specimen chuck using a freezing compound and the cryobar Note To ensure optimal adhesion of the specimen to the chuck store the chucks at room temperature prior to use Press the Cryobar button on the Main screen to activate it The Cryobar button is highlighted in blue when the function is activated To turn off the Cryobar f...

Page 47: ...to the right to toggle between Fine and Trimming thickness settings Fine Section Thickness Trim Section Thickness Note The selected function is displayed highlighted on the Main screen Turn the joystick knob left to decrease the selected thickness Turn the joystick knob right to increase the selected thickness For details of the thickness steps see Technical Specifications ...

Page 48: ...00μm 200μm 100μm or 10μm or adjust the value using the arrow keys For details of the thickness steps see Technical Specifications Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes To set the fine section thickness Press the Fine Section Thickness button on the Main screen The Fine Section screen will be...

Page 49: ... without saving the changes Temperature Both the specimen head and the blade holder are actively cooled and the user can adjust either temperature independently Setting the temperature of the specimen head Press the Specimen Head Temperature button on the Main screen The Temperature Specimen Head screen will be displayed Either select one of the preset temperatures 40 C 30 C 20 C or 10 C or adjust...

Page 50: ...re button on the Main screen The Temperature Blade screen will be displayed Either select one of the preset temperatures 35 C 30 C 20 C or 10 C or adjust the temperature using the arrow keys Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes Note The new set temperature is displayed in white on the Main ...

Page 51: ...peration 41 Cutting Window The cutting window allows the user to customise this setting for each specimen On the Main screen press the Cutting Window button The Set Cutting Window screen will be displayed ...

Page 52: ...window Continue turning the handwheel clockwise so that the upper edge of the specimen is positioned just below the blade edge Press the End button The End button is highlighted in yellow Next Steps Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes Press the Start Stop button on the right control panel ...

Page 53: ...ng of sections Notes The user sets the desired value depending upon the intended function stretching or suction process section thickness and the size of the specimen The vacuum applied for stretching sections and or disposing of section waste is only active when within the cutting window Outside of the cutting window the selected vacuum is turned off by means of a valve After 10 minutes of inacti...

Page 54: ...screen The Vacutome screen will be displayed Activate the Vacutome To activate the Vacutome Press Vacutome to display the Vacutome settings screen Use the arrow keys to increase or decrease the Vacutome suction value the default setting is 100 ...

Page 55: ... the suction value in 1 increments Turn the Joystick to the right to increase the suction value in 1 increments Note When the Vacutome is activated the Vacutome button on the Main screen will appear yellow Turning Off the vacutome function Press the yellow Vacutome button on the Main screen Press the yellow Vacutome button on the Vacutome screen the button will now appear white Press OK to confirm...

Page 56: ...Cleaning Mode button turns yellow to indicate that this function is now activated The cleaning mode will switch of automatically after 10 minutes Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes Remove the Vacutome hose from the blade holder and use it to remove any section waste from the cryo chamber ...

Page 57: ... the Main screen Press the yellow Cleaning Mode button on the Vacutome settings screen the button will now appear white Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes Re attach the Vacutome hose to the blade holder ...

Page 58: ...t Take care that the power cord is free to move To adjust the height of the cryostat Press the Height button on the touch screen A sliding menu will appear Use the arrow buttons to raise or lower the cryostat to the required level Press OK to confirm the adjustment The sliding menu disappears after approximately 3 seconds if OK is not pressed Note Continuous use of the height adjustment for longer...

Page 59: ...ton 2 Cutting Mode 4 Brake Time and Date To toggle between Time or Date indication Press the Time Date button Note When there is an outstanding service message a wrench symbol is show instead of the Date Time button Press the Wrench symbol to see the service message Cutting Mode Selection The cutting mode selection aids in utilizing the motorized cutting function Four cutting modes for the motoriz...

Page 60: ...gency stop button or unlock the emergency stop in the foot pedal The cutting mode can be changed in three ways Via the Main screen in the cutting mode submenu or via the mode button on the right touch panel To switch between the cutting modes using the Main screen Press the Multi Function button on the Main screen to switch between the cutting modes To switch between the cutting modes using the cu...

Page 61: ...e button The Menu Cutting Mode screen will be displayed Press on the desired cutting mode Continuous Interval Single or Multi Press OK to confirm the setting and to return to the Main screen Press the Back button to exit back to the Main screen without saving the changes ...

Page 62: ...left touch panel The actual cutting mode is displayed on the multi function bar on the Main screen Mode Single By using this mode a single full cutting movement is carried out with the specimen head stopping in the down position Press either the Start Stop button twice or press the foot pedal twice to carry out a single cutting stroke ...

Page 63: ...pedal twice Hold the Start Stop button or the foot pedal on the second press Release either the Start Stop button or the foot pedal to stop cutting immediately Mode Multi Stroke A preset number of strokes will be carried out Note When Multi Stroke is first selected the default number of strokes is two To set the number of strokes for the Multi Stroke Mode Press the Menu button on the Main screen T...

Page 64: ...54 Press the Cutting Mode button The Menu Cutting Mode screen will be displayed Press the Multi button to highlight it Use the arrow keys to select the number of strokes ...

Page 65: ...bed above Press either the Start Stop button twice or press the foot pedal twice to start sectioning The cutting movement stops automatically after the preset number of strokes have been carried out Mode Continuous Stroke An unlimited number of strokes will be performed when this mode is selected To start the continuous stroke mode Press either the Start Stop button twice or press the foot pedal t...

Page 66: ...e Handwheel Brake Press the Brake button on the Main screen or press the Brake button on the right touch pad Note The Brake button is highlighted if the handwheel brake is activated To deactivate the Handwheel Brake Press the red highlighted Brake button on the Main screen or the Brake button on the right touch pad ...

Page 67: ...ce to the Front End Position Indicates the remaining distance to front end position of the blade holder Section Thickness Sum This button indicates the total section thickness sum of the actual mounted specimen To reset this value press this button and keep it pressed for approximately 3 seconds Note It is recommended to reset the section thickness sum if a new specimen is mounted in the specimen ...

Page 68: ...ed from 0 to 100 using the knob on the right hand site of the instrument Turning the knob to the left decreases the speed turning to the right increases the speed Note The selected speed will be used in the cutting window Outside of the cutting window a higher return speed determined by the system is used Use either the operating controls or the foot pedal if fitted to turn on off the drive The cu...

Page 69: ...s the Section Counter button for approximately 3 seconds to reset it to zero Menu The Menu button provides access to submenus for adjustments settings and the user guide To enter the Menu screen Press the Menu button The Menu screen will be displayed Press the Back button to return to the Main screen Press the desired function button to access the respective submenu Chamber Light To adjust the bri...

Page 70: ...es Note The chamber illumination can be adjusted from the lowest 1 to the highest 100 level Standby When the CryoStar NX70 is switched on but has been inactive for 1 hour it will automatically enter the Standby mode After 3 more hours the instrument enters the sleep mode The cryostat maintains the user adjusted temperatures for both blade holder and specimen head during Standby When the cryostat e...

Page 71: ...ndby button to switch the instrument immediately into the Standby mode Press the touchscreen to exit the Standby or Sleep mode Display Lock Note The display lock is used to avoid unwanted changes to the settings of the cryostat To activate the Display Lock Press the Menu button on the Main screen The Menu screen will appear ...

Page 72: ...mperature The user can program three favorites to allow quick selection of the set of temperatures most often used Note Whenever a temperature setting is altered the cryostat will require a period of time to reach the set temperature Observe the readings on the display and begin sectioning when the preset value is reached To program Favorites Press the Menu button on the Main screen The Menu scree...

Page 73: ...four sections from left to right Favorite name displayed on the main screen if highlighted Specimen head temperature Blade temperature Alternative indication displayed on the main screen if highlighted To change the Favorite name Press the name of the preset to be altered The Keyboard screen will appear Use the upper left button to toggle between capital letters small letters and numbers ...

Page 74: ...without saving the changes Note If the value for either the specimen head temperature or blade temperature is changed on the Main Screen the favorite is no longer displayed but settings are still stored in memory User Guide This function contains an electronic version of this user guide To activate the User Guide Press the Menu button on the Main screen The menu screen will appear Press the User G...

Page 75: ...tton to return to the first page of the User Guide Use the arrow keys to browse forward or backwards through the User Guide Press the Back button to return to the settings menu screen Press the Back button twice to return to the Main screen Settings Menu To enter the Settings menu Press the Menu button on the Main screen ...

Page 76: ...66 The menu screen will appear Press the Settings button The Menu Settings screen will appear Press the Back button to return to the Menu screen Press the Back button twice to return to the Main screen ...

Page 77: ...s button The Menu Settings screen will appear Press the Language button The Menu Settings Language screen will appear Select the desired language by pressing the respective button Press OK to save the changes and to return to the Main screen Press the Back button three times to return to the Main screen without saving the changes ...

Page 78: ... must finish before the time or date can be adjusted Press the Menu button on the Main screen The Menu screen will be displayed Press the Settings button The Settings Menu screen will be displayed Set the Date Press the Date button The Menu Settings Date screen will appear ...

Page 79: ...ss the Month button to highlight it Use the arrow keys to select the desired month Set the Year Press the Year button to highlight it Use the arrow keys to select the desired year Press OK to confirm the setting and to return to the Main screen Press the Back button three times to exit back to the Main screen without saving the changes ...

Page 80: ...s OK to confirm the setting and to return to the Main screen Press the Back button three times to exit back to the Main screen without saving the changes Setting the Time Press the Menu button on the Main screen The Menu screen will be displayed Press the Settings button The Menu Settings screen will appear ...

Page 81: ...Set the minutes Press the Minute button to highlight it Use the arrow keys to select the desired minute Set the hour Press the Hour button to highlight it Use the arrow keys to select the desired hour Set the time format Press the 12 24 button to toggle between the 12 and 24 hour modes ...

Page 82: ...utton three times to exit back to the Main screen without saving the changes Start Time The Start Time is the time were the cryostat switches from the sleep mode to the Active mode on every programmed day To set the Start Time Press the Menu button on the Main screen Press the Settings button The Menu Settings screen will appear Press the Start Time button ...

Page 83: ...specify the day s and the exact time when the cryostat should start automatically Note The preset start time is from Monday to Sunday at 06 00 a m To set the Day Press the Day button The Menu Settings Start Time screen will appear Note The default setting is every day from Monday to Sunday ...

Page 84: ...eekdays will turn white Press the Hour and Minute button and use the arrow keys to adjust the time at which the cryostat should start When all settings have been done press OK to save the settings and to return to the Main screen or press the Back button three times to return to the Main screen ...

Page 85: ... MALFUNCTIONS OF THE INSTRUMENT THE SPECIMEN MIGHT BE DAMAGED Notes Set the time of the daily defrosting on the control panel The period of the defrosting takes approximately 1 hour and varies according to the thickness of the frost Possible frost built up on the microtome or on the knife carrier are not removed by defrosting the instrument However the frost recedes after defrosting as now the eva...

Page 86: ...layed Press the Back button three times to return to the Main screen without starting the defrosting Press the Now button to start the defrosting immediately The Defrosting screen will appear showing the progress of the defrosting and the actual temperature of the evaporator ...

Page 87: ...he defrosting Press the Abort button on the Defrosting progress screen The Main screen will appear displaying an error message Press the OK button to remove the error message Note The Defrosting cycle can be aborted at any time ...

Page 88: ...l working hours to avoid delays in the workflow To program the Daily Defrost Cycle Press the Menu button on the Main screen The Menu screen will be displayed Press the Settings button The Menu Settings screen will appear Press the Defrost button The Menu Settings Defrost screen will be displayed Press the Time button twice ...

Page 89: ...g press the Hour and or the Minute button Adjust the time by using the arrow keys Press the OK button to confirm your settings Press the Back button three times to return to the Main screen Screen The brightness of the Screen can be adjusted To set the Screen brightness Press the Menu button on the Main screen The Menu screen will be displayed Press the Settings button ...

Page 90: ...ayed Select one of the preset values 25 50 75 or 100 or adjust the brightness using the arrow keys Press OK to confirm the setting Press the Back button three times to return to the Main screen without saving the changes Note The chamber illumination can be adjusted from the lowest 1 to the highest 100 levels ...

Page 91: ...Press the Settings button The Menu Settings screen will appear Press the Retraction button The Menu Settings Retraction screen will be displayed Press the OK button to turn off the Retraction and to return to the Main screen Press the Back button three times to return to the Main screen without saving the changes To turn on the Retraction Enter the Retraction menu screen as described above ...

Page 92: ...ton three times to return to the Main screen without saving the changes Data Log The Data Log records all events and error messages on the cryostat The Data Log file can be exported to an USB Stick for Service purposes in case of a malfunction To enter the Data Log Press the Menu button on the Main screen The Menu screen will be displayed ...

Page 93: ...e Data Log Insert the USB Stick Note The Export button is now highlighted in white Press the Export button to transfer the log data to the USB Stick Press the Back button three times to return to the Main screen Note The Data Log file can only be exported to the USB Stick supplied with the instrument Default Settings If required the instrument can be reset to the default settings To restore the De...

Page 94: ...ings Default Settings screen will be displayed Press the OK button to restore the default settings and to return the Main screen Press the Back button three times to return to the Main screen without restoring the default settings Note A message appears on the Main screen to inform the user that the default settings have been restored The message disappears after approximately 3 seconds ...

Page 95: ...ter the Menu Settings Default Settings screen as described above Uncheck the box and press the Back button three times to save the changes and to return to the Main screen WARNING PRESSING OK WILL RESET THE INSTRUMENT TO THE DEFAULT SETTINGS Service This submenu is only for service purposes A password is required to enter the Service menu ...

Page 96: ...tton The motorized cutting drive can then be started again Optional Foot Pedal Emergency Stop A second emergency stop device is integrated into the optional foot pedal To activate the emergency stop using this method Firmly step on the foot pedal to immediately stop the motorized cutting drive Notes The button for the handwheel brake does not light up when the foot pedal emergency stop is activate...

Page 97: ...e located on the left side inside the cryo chamber These specimen stations passively cool specimens to a temperature of 30 C and can be used as a staging area The temperature range of the specimen head is adjustable between 10 C and 50 C The instrument is also equipped with an active cryobar The cryobar area actively cools down to 60 C Note Use the heat extractor to accelerate the freezing of the ...

Page 98: ...cimen being damaged during the return stroke of the specimen head The retraction can be switched off see Switching the Retraction On Off The retraction distance required is 20 microns and cannot be changed Trimming and First Cuts After the specimen orientation and the blade angle have been set trimming can be carried out using the trimming function ...

Page 99: ...down the Trim button and turn the hand wheel in a clockwise direction to begin trimming Note The blade holder will move forwards by the preset trim thickness value every cycle Sectioning and Taking Off Sections Set the desired section thickness see Cutting Thickness Set the desired vacuum level see Vacutome and Cleaning Mode Place the anti roll plate against the blade ...

Page 100: ...ection slides into the space between the blade and the anti roll plate Use the handle to move the anti roll plate assembly away from the specimen The section should remain on the clamping plate Arrange specimen section gently with a brush Transfer the specimen onto a glass microscope slide with help of adhesion forces ...

Page 101: ...D WHEEL BRAKES MUST ALWAYS BE ACTIVATED WHEN WORKING ON THE SPECIMEN HEAD OR BLADE CARRIER Electronic Handwheel Brake To activate the electronic Handwheel Brake Press the Brake button on the Main screen The Brake button is highlighted in red to indicate that the electronic brake is active Note The motorized cutting drive cannot be started if the electronic Handwheel brake is activated To release t...

Page 102: ...trument is turned off and the electronic handwheel brake is inoperable To mechanical handwheel brake can be activated by a switch on the handwheel To activate the mechanical Handwheel Brake Bring the handwheel handle into a 12 o clock position The switch for the handwheel brake can now be pressed into the locked position ...

Page 103: ...sed and move the handwheel slowly forward and backwards until the switch is locked Starting the cutting motor drive is not possible when the instrument is turned off or when the mechanical hand wheel brake is activated To unlock the mechanical Handwheel Brake Pull out the Handwheel Brake switch ...

Page 104: ...CHAMBER MUST ALWAYS BE CLOSED BEFORE STARTING AND DURING A COLD D CYCLE Note If the window is not closed the warning Window not closed will be displayed WARNING BEFORE STARTING THE COLD D FUNCTION REMOVE ALL SPECIMENS FROM THE CRYO CHAMBER Press the Disinfect button on the Main screen The Disinfection screen will appear Press OK to start the disinfection immediately Press the Back button to return...

Page 105: ...d D cycle will normally evaporate from the cryo chamber within a period of three hours The liquid droplets can be removed with a paper towel and if necessary any icing can be removed with an alcohol spray Neither the liquid droplets nor the icing will prevent the use of the cryostat immediately after completion of a Cold D cycle Any occasional build up of crystalline material in the cryo chamber c...

Page 106: ... adequately disinfect the cryo chamber However the instrument can be used immediately afterwards Programming Cold D In addition to the instant use automatic Cold D cycles can be programmed Follow the directions below to activate or deactivate the automatic Cold D feature Press the Menu button on the Main screen Press the Settings button The Settings Menu screen will be displayed ...

Page 107: ...y s and the specific time when the Cold D process should automatically be carried out To set the Day Press the Day button The Menu Settings Disinfection day selection screen will appear Note The default setting is every day from Monday to Sunday at 3 00 am Press the weekdays on which the automatic disinfection should not be carried out ...

Page 108: ...ute button and use the arrow keys to adjust the time at which the Cold D cycle should start When all settings have been done press OK to save the settings and to return to the Main screen Press the Back button to return to the Setting screen without saving the changes ...

Page 109: ... 1 Specimen is too cold Raise specimen temperature Specimen curls after lifting ARP 1 Anti Roll plate clamp plate too warm Lower chamber temperature Tip use freezer spray on clamp plate and blade to confirm 2 Static electricity in chamber 3 Blade blunt Change blade Sections tear or crack 1 Specimen too cold Raise temperature 2 Blade damaged or dirty Change blade 3 Specimen frozen too rapidly or sp...

Page 110: ...imen head jaws Check there is no debris or ice on the back of the chuck or on specimen head Sections thick thin 1 Check knife holder and specimen correctly clamped 2 Ensure specimen is securely attached to the Cryocassette chuck 3 Temperature of specimen incorrect Raise or lower temperature 4 Blade not sharp Change blade ...

Page 111: ...to the cutting temperature Adjustment of proper clearance angle Select a clearance angle of 8 12 The preset clearance angle is 10 Select an appropriate cutting speed The harder the material the slower the cutting speed Take care when bringing knife and specimen together WARNING DURING DEFROSTING REMOVE THE SPECIMENS FROM THE CRYO CHAMBER AS THE TEMPERATURE INSIDE THE CHAMBER WILL INCREASE DO NOT L...

Page 112: ... to 40 C 30 C or below Cervix 25 C to 30 C 20 C Connective Tissue 25 C 16 C Gut 25 C 20 C Heart 25 C 18 C Kidney 25 C 15 C Lip 25 C 13 C Liver 25 C 13 C Lung 25 C 15 C Lymph Node 25 C 13 C Muscle 25 C 16 C Omentum 35 C to 40 C 35 C Ovary 25 C to 30 C 20 C Pancreas 25 C to 30 C 20 C Prostate 30 C 20 C Skin 25 C 16 C Skin with fat 30 C to 35 C 25 C Spleen 25 C 16 C Testis 25 C 10 C Thyroid 25 C 15 C...

Page 113: ...e For this remove the vacuum hose from the front side of the blade carrier Attach the cleaning nozzle to the vacutome hose Remove the filter unit and dispose of the vacutome filter together with the section waste and or the micro filter according to the respective lab regulations The warming up of the microtome chamber can actively be accelerated by softly using a hair dryer Clean wash and dry the...

Page 114: ... Notes The capacity of the bottle is 2 5 Liters approximately 0 66 Gallons It is recommended to empty and disinfect the bottle once a week during the routine cleaning procedure To empty the Defrosting Liquid Bottle Open the front door and pull out the bottle from the rack Dispose of the liquid in accordance to the laboratory regulations and clean it Note After cleaning the bottle it is recommended...

Page 115: ...th the vacutome function WARNING Section waste can be hazardous Dispose of the used vacutome filter bag in accordance to the laboratory regulations The Vacutome Filter Bag is placed under the cover on the right hand site of the cryo chamber Open the lever of the cover Remove the vacutome hose 1 and lift off the cover 2 ...

Page 116: ...flexible brush Slip off the old vacutome filter and replace it by a new one Clean and disinfect the empty vacutome filter chamber with disinfectant Replace the cover rear end first Reattach the vacutome hose Lock the cover with the lever ...

Page 117: ...urn the pin counter clockwise to increase the tension of the moveable clamping jaw By turning the pin counter clockwise a click can be heard as a pin moves over the teeth on the ratchet wheel After two clicks stop turning and check the clamping tension with a specimen chuck If the tension is not sufficient increase the tension by a further click and re test the clamping tension Repeat this procedu...

Page 118: ...e defrosting and Cold D liquids in accordance to the laboratory regulations and clean the bottle see Emptying the Defrosting and Cold D Liquids Remove the defrosting liquid bottle Note Clean and disinfect all accessories according to the respective applicable lab regulations and transport them in dry condition WARNING ANY TRANSPORTATION OF THE INSTRUMENT REQUIRES ORIGINAL PACKAGING MATERIALS Note ...

Page 119: ...shelf the blade holder and all other accessories as well as tools from the cryo chamber Dispose of the defrosting and Cold D liquids in accordance to the laboratory regulations and clean the bottle see Emptying the Defrosting and Cold D Liquids Remove the defrosting liquid bottle Note Clean and disinfect these accessories according to the respective applicable lab regulations and transport them in...

Page 120: ...110 Appendices ...

Page 121: ...Extractor Specimen Chucks Ø 20 mm 715530 Ø 30 mm 715600 Ø 40 mm 715610 Ø 60 mm 715620 Cryo Molds 10 mm 570400 15 mm 570380 22 mm 570390 Brushes Brush 334000 Small brush beveled 334180 Large brush 334170 Neg 50 Freezing Medium 2 x 118 ml bottles 161425 6 x 118 ml bottles 161426 Sanosil S010 1L 175200 Cryostat Disinfectant Protect II 350270 Coarse filter for Vacutome 281080 Nozzle for trimming Blade...

Page 122: ...m 152230 Disposable Blades High Profile Blades 50 pcs in dispenser 152450 Low Profile Blades SEC 35 50 pcs in dispenser 152200 Low Profile Blades SEC 35e 50 pcs in dispenser 152215 Low Profile Blades SEC 35p 50 pcs in dispenser 152570 Paraffin repellent PARA GARD 100 ml 350170 Lubrication Oil 1 x 100 ml bottle 350110 1 x 250 ml bottle 350120 ...

Page 123: ... eye wear Remove all disposable blades and knives from the chamber and disinfect them separately Using a paper towel remove all tissue trimmings and waste material from the cryostat chamber Bring the cryostat chamber to room temperature before beginning the disinfection process Use 3 Sanosil as the disinfection agent Spray the solution throughout the chamber let sit for 20 minutes drain and then w...

Page 124: ...ting Movement 90 Cutting Speed 7 16 59 103 Cutting Window 7 8 36 43 D Data Log 84 Date 36 70 Defrost 8 77 80 E Emergency Stop Button 14 52 88 F Favorites 8 45 64 H Heated Window 35 77 Height Adjustment 5 36 50 J Joystick 5 7 8 16 39 L Language 8 69 Lock 11 63 94 109 M Multi Function Bar 36 51 Multi Function Button 36 59 P Power Save 63 74 R Reservoir 8 14 33 Retraction 7 8 83 90 S Section Counter ...

Page 125: ...Index 115 W Waste Tray 16 ...

Page 126: ...116 ...

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Thermo Scientific CryoStar NX50, Operator'S Manual

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