2
– Installation
Thermo Scientific
Product Manual for CarboPac PA200 Columns
Page 8 of 37
065734-01
For Research Use Only. Not for use in diagnostic procedures.
2.
Installation
Read the instrument manuals. This manual assumes that you are using Thermo Scientific
Dionex instrumentation and are familiar with the installation and operation of the Thermo
Scientific Dionex Ion Chromatograph (IC). If you do not understand the operation of the
system, take the time to familiarize yourself with the various system components before
beginning an analysis.
The proper configuration of an Ion Chromatography System (ICS) is dependent on column
format. Although they can typically use the same system, the use of a 1-mm or 0.4-mm column
requires different set up to ensure maximum performance. Under the Dual EG mode, a high-
pressure isocratic pump is required for applications for both 1-mm and 0.4-mm column formats.
For high pressure applications, the use of high pressure fittings is recommended.
2.1
The Dionex High Pressure Ion Chromatography Systems
A minimum of a Dionex High Pressure Ion Chromatography System (HPIC) is recommended due
to the higher backpressures generated at typical operational flow rates. Systems should have the
capability to operate up to at least 5000 psi. Standard IC systems, with an upper limit of 3000 psi,
are insufficient for proper column operation.
It is highly recommended to ensure that the systems used for carbohydrate analysis are metal-free.
Metal ions from a metal system will contaminate the Dionex CarboPac column and may also
contaminate the working electrode. Running a Dionex CarboPac column on a metal system voids
the column warranty.
Care should always be taken not to exceed the maximum operating pressure of the system
component. ICS systems with lower backpressure capabilities are not recommended as
reduced flow rates may result in loss of performance.
Contact your local representative for information on how to customize your system to
your application needs.
NOTE
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WARNING
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NOTE
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