4
– Applications
Thermo Scientific
Product Manual for CarboPac PA200 Columns
Page 24 of 37
065734-01
For Research Use Only. Not for use in diagnostic procedures.
4.3
Profiling of Galacto-oligosaccharides
Galacto-oligosaccharides (GOS) are non-digestible neutral carbohydrates with the ability to
manipulate the composition of colonic microflora in order to improve the gastrointestinal health.
These carbohydrates are enzymatically produced by transgalactosylation reactions of lactose
catalyzed by β-galactosidases to give rise to galactose oligomers with a terminal glucose, with
different glycosidic linkages and degrees of polymerization (DP). Depending on the enzymatic
source used for their synthesis, the chemical structure of these oligosaccharides varies and,
consequently, their effect on gut microflora can change. The characterization of GOS structures
is a required and important task to understand their mechanism of action on human gut.
The CarboPac PA200, 3 mm column (Document No. 031992) is NOT compatible with the ICS-
6000 Dual EG mode but it is recommended to be used with manually-prepared sodium
acetate/sodium hydroxide eluents. The illustration of the application on the CarboPac PA200, 3
mm column is for comparison only, to demonstrate the comparable performance of the CarboPac
PA200, 1 mm and 0.4 mm columns with the ease of use.
Columns:
CarboPac PA200, 3-mm (guard + separator)
CarboPac PA200, 1-mm (guard + separator)
CarboPac PA200, 0.4-mm (guard + separator)
Gradient:
CarboPac PA200, 3-mm:
-5 min: 50 mM NaOAc in 100 mM NaOH
0-45 min: 50-150 mM NaOAc in 100 mM NaOH
45-55 min 500 mM NaOAc in 100 mM NaOH
55-60 min: 50 mM NaOAc in 100 mM NaOH
CarboPac PA200, 1-mm:
0-45 min: 20-70 mM KMSA in 70 mM KOH
45-55 min: 100 mM KMSA in 70 mM KOH
55-70 min: 20 mM KMSA in 70 mM KOH
CarboPac PA200, 0.4-mm
0-45 min: 18-65 mM KMSA in 70 mM KOH
45-55 min: 100 mM KMSA in 70 mM KOH
55-70 min: 18 mM KMSA in 70 mM KOH
Flow Rate:
CarboPac PA200, 3-mm: 0.5 mL/min
CarboPac PA200 1-mm: 0.063 mL/min
CarboPac PA200 0.4-mm: 0.010 mL/min
Detection:
CarboPac PA200, 3-mm: PAD, Au on PTFE, 2 mil gasket, Ag/AgCl ref.
CarboPac PA200, 1-mm: PAD, Au on PTFE, 1 mil gasket, Ag/AgCl ref.
CarboPac PA200, 0.4-mm: PAD, Au on PTFE, 1 mil gasket, Ag/AgCl ref.
Samples:
CarboPac PA200, 3-mm: 50 mg/L GOS (Bimuno GOS powder)
CarboPac PA200, 1-mm: 5 mg/mL GOS (Bimuno GOS powder)
CarboPac PA200, 0.4-mm: 5 mg/mL GOS (Bimuno GOS powder)
