Thermo Scientific CarboPac PA200, Product Manual

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3 – Operation
Thermo Scientific Product Manual for CarboPac PA200 Columns Page 16 of 37
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For Research Use Only. Not for use in diagnostic procedures.
3. Operation
3.1 Dionex CarboPac PA200 column Operational Parameters
 pH 0-14
 Temperature limit: 4-60 °C
 Pressure limit: 4500 psi
 Organic Solvent Limit: 100% compatible
 Typical Eluents: potassium hydroxide, potassium methanesulfonate
 Standard Flow Rate: 1 mm: 0.063 mL/min
0.4 mm: 0.010 mL/min
3.1.1 The Best Operational Guidelines
3.1.1.1 ALWAYS…
 Use dedicated glass- and plastic- ware for sample handling.
 Use high purity water (>18.2 MΩ-cm resistivity).
 Keep your water eluent bottle blanketed with ~ 5 psi helium or nitrogen. Use new filtered
water if left un-blanketed for more than 30 minutes.
3.1.1.2 NEVER…
 Proceed to a next installation step if the previous step has failed.
 Start an installation with any of the check list items below missing.
 Use ‘communal’ filtration units or filters made of unknown or unsuitable (e.g., cellulose
derivatives) materials.
 Use MeOH or other organic solvents as rinse fluid in the autosampler. Use only water,
replaced daily, or use sampler wash bottles blanketed with ~3 psi Nitrogen or helium.
 Run above 60 °C or 5000 psi.
3.1.2 Initial Check List
The following items MUST be available in your lab. The absence of any of these may compromise
your analysis.
 Laboratory water unit delivering >18.2 megohm-cm water at the installation site.
 Vacuum system for eluent vacuum filtration
 Inert gas cylinder (helium or nitrogen) with a regulator valve (for example, a 0-200 psi
gauge on the low pressure side) and the appropriate size adaptors plus tubing
 Plastic eluent bottles with gas-tight cap-fittings.