Thermo Scientific CarboPac PA200, Product Manual

Page: 5 / 37

Contents
Thermo Scientific Product Manual for CarboPac PA200 Columns Page 5 of 37
065734-01
For Research Use Only. Not for use in diagnostic procedures.
Contents
1. Introduction ......................................................................................................... 7
1.1 CarboPac PA200 ............................................................................................................................................ 7
2. Installation ........................................................................................................... 8
2.1 The Dionex High Pressure Ion Chromatography Systems ............................................................................. 8
2.2 System Configuration .................................................................................................................................... 9
2.3 System Requirements................................................................................................................................... 10
2.3.1 Installation of Disposable Electrode into a Dionex ICS-5000 ED Cell,
pH-Ag/AgCl Reference Electrode or PdH Reference Electrode ......................................................... 10
2.3.2 System Void Volume .......................................................................................................................... 10
2.4 The Injection Volume .................................................................................................................................. 11
2.4.1 The 1 mm System Injection Volume, 0.4 µL ...................................................................................... 11
2.4.2 The 0.4 mm System Injection Volume, 0.1 µL ................................................................................... 11
2.5 The Dionex CarboPac PA200 Guard Column ............................................................................................. 11
2.6 System Start-up ............................................................................................................................................ 11
2.6.1 ICS-6000 Dual EG Start-Up Procedure for CarboPac PA200, 1 mm Column ................................... 12
2.6.2 ICS-6000 Dual EG Start-Up Procedure for CarboPac PA200, 0.4 mm Column ................................ 13
2.6.3 Verification of Column Cleanliness .................................................................................................... 15
3. Operation ...........................................................................................................16
3.1 Dionex CarboPac PA200 column Operational Parameters .......................................................................... 16
3.1.1 The Best Operational Guidelines ........................................................................................................ 16
3.1.2 Initial Check List ................................................................................................................................. 16
3.2 Purity Requirements and Preparation of Eluents and Standards .................................................................. 17
3.2.1 Deionized Water.................................................................................................................................. 17
3.2.2 Carbohydrate Sialyated N-linked Alditol Standard ............................................................................. 17
3.3 Sample Preparation ...................................................................................................................................... 17
3.4 Method Development................................................................................................................................... 18
3.5 System Re-start ............................................................................................................................................ 18
3.5.1 System Re-Start Procedure for Dionex CarboPac PA200, 1 mm Column .......................................... 18
3.5.2 System Re-Start Procedure for Dionex CarboPac PA200 0.4-mm Column ........................................ 19
4. Applications .......................................................................................................20
4.1 Fetuin Oligosaccharide Alditol Profiling ..................................................................................................... 20
4.2 Profiling of Inulin ........................................................................................................................................ 22
4.3 Profiling of Galacto-oligosaccharides .......................................................................................................... 24