5
– Troubleshooting Guide
Thermo Scientific
Product Manual for the ERS 500 Suppressor
Page 61 of 84
031956-11
For Research Use Only. Not for Use in Diagnostic Procedures.
5.2
Small or Increasing Analyte Peak Areas
This problem is caused by running eluent through the Dionex ERS 500, Dionex ERS 500e, or
Dionex AERS 500 Carbonate with the power off while using the AutoSuppression Recycle
Mode or the AutoSuppression External Water Mode. It may also be caused by application to too
little current to the suppressor for an extended period of time. A regeneration protocol is needed
to ensure good operation.
The Dionex AERS 500 Carbonate suppressor has different electrical current requirements
than the Dionex AERS 500 and Dionex ERS 500e, suppressors. If an AERS_Carbonate
mode is not available the current applied to a Dionex AERS 500 Carbonate should be
adjusted by a factor of 1.30 compared to a standard AERS 500 suppressor. Chromeleon 7.2
SR3 MUa and later versions have an option for selecting an AERS_Carbonate suppressor
in the Instrument Method Wizard and Editor dialog box. Selecting this suppressor from the
drop-down list will automatically apply the recommended current setting for Dionex AERS
500 Carbonate suppressors.
5.2.1
Suppressor Chemical Regeneration Steps
For fast regeneration it is recommended to follow the steps below for Chemical Regeneration of
the suppressor.
A.
Disconnect the eluent line from the analytical column attached to the ELUENT IN port
of the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate at the
analytical column end of the line. Direct this line to a separate waste beaker.
B.
Disconnect the eluent line from the ELUENT OUT port of the suppressor.
C.
For the Dionex AERS 500, Dionex AERS 500e or Dionex AERS 500 Carbonate:
Using a plastic syringe push approximately 3 mL of 200 mN H
2
SO
4
through the
ELUENT OUT port and approximately 5 mL of 200 mN H
2
SO
4
through the REGEN
IN port. Flush with 3 mL degassed, deionized water through the ELUENT OUT port
and 5 mL degassed, deionized water through the REGEN IN port.
D.
For the Dionex CERS 500 or Dionex CERS 500e:
Using a plastic syringe slowly push approximately 3 mL of freshly prepared 200 mN
NaOH (made up using degassed, deionized water) through the ELUENT OUT port and
approximately 5 mL of 200 mN NaOH through the REGEN IN port. Flush with 3 mL
degassed, deionized water through the ELUENT OUT port and 5 mL degassed,
deionized water through the REGEN IN port.
E.
Reconnect the eluent line from the ELUENT IN port of the suppressor to the analytical
column and the eluent line from the eluent out port of the suppressor to the
conductivity detector cell.
F.
If you are in the Auto Suppression Recycle mode of operation, begin pumping eluent
and immediately turn on the power. DO NOT LET THE ELUENT FLOW THROUGH
THE SUPPRESSOR FOR MORE THAN A FEW MINUTES WITHOUT TURNING
ON THE POWER.
If you are in the Auto Suppression External water mode of operation, establish water
flow through the regenerant chambers, begin pumping eluent and immediately turn on
the power. DO NOT LET THE ELUENT FLOW THROUGH THE SUPPRESSOR
FOR MORE THAN A FEW MINUTES WITHOUT TURNING ON THE POWER.
NOTE
!
