4
– Example Applications
Thermo Scientific
Product Manual for the ERS 500 Suppressor
Page 47 of 84
031956-11
For Research Use Only. Not for Use in Diagnostic Procedures.
4.6
Anion Example Applications
4.6.1
Dionex IonPac
®
AS11 Gradient
A.
Without Guard
Eluent 1:
Type I DI Water
Eluent Flow Rate:
2.0 mL/min
Eluent 2:
5.0 mM NaOH
Eluent 3:
100 mM NaOH
TIME (min)
% E1
% E2
%E3
Comments
Equilibration
0
90
10
0
0.5 mM NaOH for 7 min
7.0
90
10
0
Analysis
0.0
90
10
0
0.5 mM NaOH, Inject
0.2
90
10
0
Inject Valve to Load Position
2.0
90
10
0
0.5-5.0 mM NaOH in 3 min
5.0
0
100
0
5.0-38.25 mM NaOH in 10 min
15.0
0
65
35
B.
With Guard
Eluent 1: Type I DI Water
Eluent Flow Rate: 2.0 mL/min
Eluent 2: 5.0 mM NaOH
Eluent 3: 100 mM NaOH
TIME (min)
% E1
% E2
%E3
Comments
Equilibration
0
90
10
0
0.5 mM NaOH for 7 min
7.0
90
10
0
Analysis
0.0
90
10
0
0.5 mM NaOH, Inject
0.2
90
10
0
Inject Valve to Load Position
2.5
90
10
0
0.5-5.0 mM NaOH in 3.5 min
6.0
0
100
0
5.0-38.25 mM NaOH in 12 min
18.0
0
65
35
The steps for sample neutralization can be performed while the Thermo Scientific Dionex
IonPac AS11 is equilibrating with the starting eluent (90% E1/10% E2).
Seven minutes are required at the beginning of the above program for equilibration of the
Dionex IonPac AS11 with E1 prior to injecting the next sample. If the system is not used
continuously, that is, the run program (equilibration plus analysis) is not started exactly every 22
minutes (without Dionex IonPac AG11) or 25 minutes (with Dionex IonPac AG11), the run
program can be modified to start with 2 minutes of the highest eluent concentration for
regeneration and then to equilibrate with E1 for 7 minutes with the next injection 9 minutes into
the program.
NOTE
!
