3
– Operation
Thermo Scientific
Product Manual for the ERS 500 Suppressor
Page 29 of 84
031956-11
For Research Use Only. Not for Use in Diagnostic Procedures.
down to lock the Dionex ERS 500 in place. Lift up and pull out to remove the suppressor.
Ensure the suppressor is plumbed properly according to the selected mode of operation. Refer to
, “Installation,” for complete installation instructions.
Keep the regenerant chambers full with the appropriate regenerant solution or water. The
membranes and screens in the Dionex
ERS 500, Dionex ERS 500e or Dionex AERS 500
Carbonate must be completely hydrated to maintain liquid seals and chromatographic
performance.
The correct amount of back pressure on the conductivity detector for optimum operation is
40 psi. Connect the back pressure coil(s) appropriate for your column i.d. and flow rate.
Back pressures over 450 psi after the Dionex
ERS 500, Dionex ERS 500e or Dionex AERS
500 Carbonate can cause irreversible damage.
Do not cap the waste reservoir.
Hydrogen gas generated by the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500
Carbonate is not dangerous unless the gas is trapped in a closed container and allowed to
accumulate. The Gas Separator Waste Tube must be open to the atmosphere, and not in a
confined space, to operate properly.
3.2.1
Hydration
Hydrating the suppressor ensures that the ion exchange membranes are in a swollen form for
proper operation. A 20 minute static step is recommended during first time installation to ensure
complete hydration.
1.
If also installing a new guard and/or analytical column, follow the column startup
procedure(s) before moving on to Step 2. The column waste should be diverted to
waste for at least 10 column volumes before installing the new suppressor.
2.
Install the suppressor in the system and plumb the unit in the recycle mode (refer to
, below).
3.
Pump ≤10 mM eluent at the application flow rate into the suppressor for 5 minutes,
from the Eluent IN port.
The power to the suppressor must be off during this step.
Care should be taken not to exceed 100 psi of backpressure on the ERS suppressor.
The backpressure to the suppressor includes any tubing, cell and backpressure coils
that are connected to the eluent out port of the suppressor.
4.
Turn off the pump and allow the suppressor to sit for approximately 20 minutes to fully
hydrate the suppressor resin, screens, and membranes.
5.
After completion of Steps 1 through 4, normal operation may resume using the
application eluent strength and current recommendation.
WARNING
!
CAUTION
!
CAUTION
!
SAFETY
!
