3
– Operation
Thermo Scientific
Product Manual for the ERS 500 Suppressor
Page 36 of 84
031956-11
For Research Use Only. Not for Use in Diagnostic Procedures.
2.
Using a coupler (Item # 039056) and a 1/8” o.d. piece of tubing (Item # 035728)
from the Installation Kit, connect one end of this line to the REGEN OUT port of
the suppressor and then connect the other end of the line to the Gas Separator
Waste Tube.
C.
Fill the water source reservoir. Make sure that the O-ring is inside the cap of the
reservoir before screwing the cap onto the reservoir. Screw the cap onto the reservoir
tightly and place the reservoir near the Chromatography Module.
D.
With current applied
, adjust the external water flow rate to match the eluent flow rate
by using a graduated cylinder and measuring the flow from the REGEN OUT waste
line. The pressure applied to the reservoir can vary from 0–25 psi (the lower and upper
pressure limits of the water reservoir) but the typical operating pressure is between 10
–15 psi for the Dionex ERS 500, or 5 – 10 psi for the Dionex ERS 500e or Dionex
AERS 500 Carbonate suppressor. Please note that this value is highly system
dependent and may vary from one suppressor to the next. In summary, the final
external water flow rate is dependent on two factors: the pressure applied to the water
reservoir and the current setting. It is best to measure it with the current on since the
application of current can affect the final flow rate significantly.
A safety relief valve on the reservoir regulator prevents pressure greater than 25 psi from
being applied to the water reservoir.
Table 6
Recommended External Water Flow Rate for Dionex ERS 500
Recommended External Water Flow Rate
Equal to the Eluent Flow Rate
Maximum External Water Flow Rate
2 mL/min (2 mm suppressors)
5 mL/min (4 mm suppressors)
Recommended External Flow Rate
(Neutralization Mode)
5 mL/min (4 mm suppressor only)
Table 7
Recommended External Water Flow Rate for Dionex ERS 500e and Dionex
AERS 500 Carbonate
Recommended External Water Flow Rate
At least 2x the eluent flow rate
Maximum External Water Flow Rate
2 mL/min (2 mm suppressors)
5 mL/min (4 mm suppressors)
3.4.3
Regenerant Flow Path Connections in the AutoSuppression External Water Mode with
Peristaltic Pump
For peristaltic pump plumbing refer to the “MASTERFLEX
®
C/L
®
Peristaltic Pump Quick Start
Guide” (Item # 065203). For the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500
Carbonate plumbing refer to
, The AutoSuppression External Water Mode Plumbing
Diagram and pump the Deionized water into the “Regen in” port of the suppressor using the
peristaltic pump at the same flow rate as the eluent flow rate, see
SAFETY
!
