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Chapter 13: Glossary and Abbreviations

iEMS



Incubator/Shaker User Manual

Rev. 3.0, Cat. no. 1506200A

13 Glossary and Abbreviations

EIA

Enzyme immunoassay.

European Norm.

European Union.

IEC

International Electrotechnical Commission.

Incubator

An apparatus for maintaining optimal conditions (temperature, humidity, etc.) for
growth and development.

Microplate

A rigid or framed polystyrene plate with microwells in different well formats (e.g., 6,
12, 24, 48, 96, 384, 864, etc., wells) for ease of use in performing multiple tests
through techniques such as EIA or ELISA.

Orbital shaking

The shaking of a microplate in a circular movement with a fixed orbit and adjustable
speed.

rpm

Revolutions per minute.

13.1 Keywords for web pages

drug discovery

Microtiter plate

EIA microwell

plate

ELISA orbital

shaker

enzyme immunoassay

orbital shaking

immunoassay

orbital shaking incubator

incubating plate
incubator separation

technique(s)

Incubator shaker

shaker

liquid handling

shaking

microplate

solid phase assay(s)

microplate incubator

Thermo

Electron

Summary of Contents for iEMS

Page 1: ...iEMS Incubator Shaker Rev 3 0 Cat no 1506200A iEMS Incubator Shaker User Manual ...

Page 2: ...ht to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Electron assumes no liability for any errors or omissions nor for any damages resulting from the application or use of thi...

Page 3: ...nal Description 10 5 Installation 14 6 Operation 19 7 Maintenance 35 8 Troubleshooting Guide 40 9 Technical Specifications 45 10 Ordering Information 49 11 Warranty Certificate 50 12 Index 51 13 Glossary and Abbreviations 54 Appendix A iEMS Incubator Shaker Quick Reference Guide 55 Appendix B Certificate of Decontamination 56 Appendix C iEMS Incubator Shaker Feedback Form 57 Appendix D Addresses 5...

Page 4: ...ng transport 15 5 2 4 Climatic requirements 15 5 2 5 Things to avoid 16 5 2 6 Technical prerequisites 16 5 3 Setups before you put the instrument into operation 16 5 3 1 Voltage selector 16 5 3 2 Mains supply cable 17 5 3 3 Setup 18 6 Operation 19 6 1 Principle of operation 19 6 2 Programming protocols 20 6 3 Slot status control LEDs and audio signal 23 6 4 Normal operation 24 6 4 1 Placing the mi...

Page 5: ...lation 40 8 2 2 General 41 8 2 3 Temperature 42 8 2 4 Shaking 42 8 2 5 Operation 43 8 2 6 Maintenance 43 8 3 Service request protocol 44 9 Technical Specifications 45 9 1 General specifications 45 9 2 Incubator specifications 46 9 3 Shaker specifications 46 9 4 Safety specifications 47 9 5 In conformity with the requirements 48 10 Ordering Information 49 10 1 List of accessories and recommended sp...

Page 6: ...lecting the correct voltage 17 Fig 6 1 Principle of operation 19 Fig 6 2 Microplate in the thermal microplate holder 24 Fig 6 3 Inserting the thermal microplate holder into a slot 25 Fig 6 4 Pushing down the microplate holder to latch it properly 25 Fig 6 5 Removing the microplate holder lifting the handle 30 Fig 6 6 Removing the microplate holder pulling the holder out 30 Fig 7 1 Opening the volt...

Page 7: ...ON Power OFF SN Serial number REF Catalog number Date of manufacture Consult Instructions for Use Warning markings used in the documentation Warning Risk of electric shock Warning Biohazard risk Warning Hot surface risk of burns Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application Other markings u...

Page 8: ...signed to give you the information you need to Review safety precautions Install the iEMS Incubator Shaker Carry out incubating and shaking procedures Perform basic maintenance procedures Troubleshoot the instrument performance Maintain the instrument This User Manual also describes features and specifications of the iEMS Incubator Shaker hardware and on board software Chapter 6 Operation explains...

Page 9: ...bation modules Each module has three incubation slots for microplates All slots can be used for processing microplates individually within certain limitations A control LED above each slot indicates the status of that slot The keyboard is used to enter incubating and shaking parameters and to start thus programmed protocols These protocols can be the same for all slots or entered individually for ...

Page 10: ...he microplate holder For effective mixing a powerful variable speed orbital shaker is incorporated Featuring an orbit of 1 mm and speeds from 400 to 1400 rpm in 250 rpm increments the shaker motion ensures efficient mixing of even very viscous liquids All slot positions within a module are shaken simultaneously Each slot of the iEMS Incubator Shaker has a control LED light emitting diode that disp...

Page 11: ...0 Cat no 1506200A 11 4 2 iEMS Incubator Shaker rear view 1 Cooling air inlet 2 DIP switches not in use 3 Device link RS 485 not in use 4 Computer RS 232C device link RS 485 not in use 5 Cooling air outlet 6 Instrument label Fig 4 2 iEMS Incubator Shaker rear view 2 1 3 4 5 6 ...

Page 12: ...mal microplate holders to the temperature set in processing protocol parameters shake Starts shaking with the speed and time set in processing protocol parameters start Starts operation defined by processing protocol parameters 0 9 Are used for entering slot numbers and parameter values C Clears an incorrectly keyed numeric value Enters a keyed value or continues the program Fig 4 4 iEMS Incubator...

Page 13: ...tom with heating element 4 Adjustment screws 5 Handle A1 Position of the A1 well on the microplate Fig 4 5 Thermal microplate holder The thermal microplate holder is designed for holding the microplate during the incubation process ensuring accurate temperature control Note Adjust the screws Fig 4 5 item 4 with a screwdriver so that the microplate is held firmly in place during shaking 1 2 3 4 A1 ...

Page 14: ... to the mains input socket Refer to 5 3 2 5 2 What to do upon delivery 5 2 1 How to unpack Move the unpacked instrument to its site of operation Unpack the iEMS Incubator Shaker instrument and accessories carefully with the arrows on the transport package pointing upwards The following notes and instructions are sent with the instrument and are immediately available when you open the package the W...

Page 15: ...om the carrier Upon receipt of the carrier s inspection report arrangements will be made for repair or replacement Visually check all interconnections in the basic instrument Check that there are no loose parts inside the instrument If any parts are damaged contact your local Thermo representative 5 2 4 Climatic requirements When you set up your iEMS Incubator Shaker avoid sites of operation with ...

Page 16: ... instrument is 30 kg 66 lbs The instrument operates at voltages of 100 120 Vac 220 240 Vac and the frequency range 50 60 Hz 5 3 Setups before you put the instrument into operation 5 3 1 Voltage selector The voltage selector fuse block assembly Fig 5 1 is located on the left hand side Fig 4 1 item 8 of the instrument Check that the selected voltage corresponds to the local voltage You can see the c...

Page 17: ...ack into the power entry port housing Snap the assembly securely into place 5 Reinsert the power cable 5 3 2 Mains supply cable Warning Never operate your instrument from a power outlet that has no ground connection Never use a mains supply cable cord other than the Thermo mains supply cable designed for your region 1 Ensure that the mains switch Fig 4 1 item 7 at the back of the left side panel i...

Page 18: ...mware version is shown on the display Set the clock time 1 While in the READY state press C to select the setup menu 2 Select 1 for clock Press Enter 3 Program the new time hh mm ss and press 24 hour clock The baud rate is used for service purposes only If you want to interrupt the setup press stop to return to the READY state ...

Page 19: ...d slots Section 6 5 Preheating and heating before starting the operation Section 6 4 Normal operation It is also possible to start shaking the slots without heating as a separate function Section 6 6 Shaking For an overview of the operations see Fig 6 1 below Fig 6 1 Principle of operation Power ON READY shake start Shaking only SHAKING ON READY Change protocol parameters Slot no 1 9 or 0 for all ...

Page 20: ...Section 6 5 Preheating and heating Operation Display Switch the power ON Note If the power is already on any shaking or heating must be turned off in the slot to be able to program a protocol To program the incubation and shaking parameters press the appropriate slot number 1 9 For example Press 1 Enter If you want to program the same parameters to all slots Press 0 Program the new temperature val...

Page 21: ...rsor For example for a shaking time of 20 seconds Press 00 00 20 Note If you want to interrupt programming press stop to return to the READY state Your changes will be saved Interval time is a programmable parameter that allows you to specify at what interval the shaking is alternately switched off and on e g an interval time of 00 00 10 results in 10 seconds of shaking turned on 10 seconds of sha...

Page 22: ...e shaker Value 0 1 2 3 4 5 Shake speed rpm 0 400 650 900 1150 1400 Caution Splashing may occur if you use shaking with wells that contain too much liquid for a given speed To avoid splashing and potential cross contamination from well to well do not overfill wells for a given shaking speed setting When the well is filled to 50 of its nominal volume the shake speed setting should not exceed value 4...

Page 23: ...mperature has not yet been reached Orange The slot has reached the set temperature and is ready for operation Red The slot is in use shaking and or incubating Green The processing protocol is complete and the heating of the slot is still on The orange control LED flashes when a slot is heating up to the programmed target temperature The LED turns to a continuous orange color when the slot reaches ...

Page 24: ...ts cover since it will come off Use the handle Fig 6 2 Caution Do not place the microplate holder into the instrument while the instrument is shaking This may damage the shaking mechanism Fig 6 2 Microplate in the thermal microplate holder Be careful not to spill the contents of the microplate when you load it into the thermal microplate holder and when you insert the holder into the slot Carefull...

Page 25: ...ing position Caution Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism Fig 6 3 Inserting the thermal microplate holder into a slot Fig 6 4 Pushing down the microplate holder to latch it properly Note If the thermal microplate holder is not latched into position it may lose contact with the temperature contro...

Page 26: ...ng protocol The slot has reached the target temperature when the orange LED has stopped flashing and stays continuously orange Before you start the processing protocol ensure that the temperature of the slot is within the acceptable range appropriate for the application to be carried out You can view the actual slot temperature on the instrument display see Section 6 5 Preheating and heating Chang...

Page 27: ...king This may damage the shaking mechanism 6 4 3 2 Checking the operation time During operation you can check the remaining incubation time by pressing the slot number For example Press 1 If the remaining time is 23 minutes and 15 seconds the display will show Press C to return to the READY state 6 4 3 3 Starting a program during operation in another slot Within a module if a slot has a protocol i...

Page 28: ...6 4 3 5 Stopping the operation If you press a slot number and stop before the processing protocol time elapses the protocol stops and incubation stays on The LED will light up with a green color and you will hear a beeping sound For turning off the beeping sound see Section 6 4 3 6 The READY state appears 6 4 3 6 Turning off the audio signal Caution Do not place the microplate holder in or remove ...

Page 29: ...will stop and the green led and the audio signal will turn off 6 4 4 Removing the microplate Caution Do not remove the microplate holder from the instrument while the instrument is shaking This may damage the shaking mechanism 1 Wait until the slot s control LED is green and you hear the audio signal The processing protocol is complete but the heating of the slot is still on If you wish to turn th...

Page 30: ...nual Rev 3 0 Cat no 1506200A 30 Fig 6 5 Removing the microplate holder lifting the handle Fig 6 6 Removing the microplate holder pulling the holder out 3 Place the microplate holder on a heat resistant surface Open the cover and remove the microplate ...

Page 31: ...he current time of day will be shown on the display 6 5 Preheating and heating Operation Display Press temp followed by the slot number to display the temperature in a particular slot or to start the preheating of the thermal microplate holders Press temp Press the slot number 1 9 For example Press 1 The actual TRUE slot temperature and heating status HEAT OFF or HEAT ON are displayed If the therm...

Page 32: ...ng is OFF in every slot the display will show If some slots are ON and some are OFF the display will show At this point press start to turn the heating ON in all slots or press stop to turn the heating OFF in all slots Press C to return to the READY state PLACES 1 9 HEAT OFF PLACES 1 9 HEAT ON OFF READY 09 29 10 ...

Page 33: ...pressing the shake key Press shake Press the slot number 1 9 For example Press 1 To start shaking press start The shaking time and speed are the same as the parameters set in the programming phase After pressing shake you can select the slot numbers by pressing number keys or by scrolling with the Enter key Within a module if a slot has a protocol in progress with a shaking speed of 0 another prot...

Page 34: ...ters press the appropriate slot number and then press Enter Continue to press to display the programmed parameters for temperature incubation time shake time interval time and shake speed Caution You can only view programmed incubation and shaking parameters of a slot while the slot is not heating or shaking When you review the parameters ensure that you do not make any unintentional changes to th...

Page 35: ...lean low pressure compressed air or a cloth dampened with water or a mild detergent when necessary Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Clean the keyboard with a mild laboratory detergent P...

Page 36: ...power input socket 2 Use a small flat screwdriver to pry the voltage selector fuse block assembly away from the power entry port housing and remove Fig 7 1 Fig 7 1 Opening the voltage selector fuse block assembly 3 Replace the blown fuses with the same type 3 15 A See Fig 7 2 Fig 7 2 Fuse block assembly 4 Place the fuse holder back into the slot and push it in until you hear a click 5 Connect the ...

Page 37: ...h the reagents used should be followed A decontamination procedure is only recommendable when infectious substances have been in direct contact with any part s of the instrument If there is any risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended that the complete decontam...

Page 38: ... towel with 2 glutaraldehyde containing decontaminating solution and clean the surfaces of the thermal microplate holder 4 Place the disposable towels into a biohazard container If the abovementioned cleaning procedure is insufficient because of the extensiveness of the spill of infectious agent perform the following decontamination procedure 1 Place the thermal microplate holder into a container ...

Page 39: ...t or other items Enclose the return authorization number RGA given by your Thermo representative Indicate the fault after you have been in touch with your local Thermo representative or Thermo s service department See Section 9 1 for details on storage and transportation temperatures See also Section 8 3 Service request protocol 7 6 Disposal of the instrument Warning Decontaminate the instrument p...

Page 40: ...eating after you have powered on the instrument Refer to Section 6 5 Preheating and heating Module incubation temperature All slots within a module will be heated to the same temperature The slots within a module are kept at the temperature that is programmed into the first started processing protocol in the module If another temperature is programmed to another slot in the same module the program...

Page 41: ...ing with wells that contain too much liquid for a given speed To avoid splashing and potential cross contamination from well to well do not overfill wells for a given shaking speed setting When the well is filled to 50 of its nominal volume the shake speed setting should not exceed value 4 It is recommended to use the setting 3 in most cases Refer to Section 6 2 Programming protocols Splashing aer...

Page 42: ... processing protocol from starting if the slot is not at the target temperature You can view the actual slot temperatures on the instrument display Refer to Section 6 5 Slot warmup time Allow the slots to reach the programmed target temperature before you start any processing protocol The slot has reached the target temperature when the orange LED has stopped flashing and stays continuously orange...

Page 43: ...n the power off to the instrument and unplug the power cable during maintenance procedures Dirty thermal microplate holder contacts Ensure that the power contacts on the back of the thermal microplate holder are clean and free from dirt or corrosion Dirty contacts may affect temperature control of the microplate holder in the slot Corrosive materials Do not use corrosive materials during maintenan...

Page 44: ...ke care of sending a complaint form Complaint order to Thermo s service department The Complaint order contains a more detailed description of the fault symptom or condition Give all the necessary information to the distributor who will fill in and forward the Complaint order to Thermo s service department Check Section 7 5 How to pack for service You will find instructions on how to proceed befor...

Page 45: ...Mains power supply 100 120 Vac 60 Hz nominal 200 240 Vac 50 Hz nominal Power consumption 380 VA max Fuses 2 x T3 15 A 250 V 5 x 20 mm IEC 127 3 III Operating conditions indoor use 10 C 40 C RH 90 Transportation conditions 40 C 70 C packed in transport packaging Storage conditions 25 C 50 C packed in transport packaging Microplate dimensions Maximum 86 mm W x 128 mm L x 15 mm H Device interfaces 1 ...

Page 46: ... 37 C with an ambient temperature of 24 C with 200 µl water well without a plate sealer and without shaking Uniformity 0 3 C across the entire plate calculated as the difference between the maximum and minimum well temperatures of wells A1 A12 H1 H12 and E7 after 1 hour at 37 C with an ambient temperature of 24 C with 200 µl water well without a plate sealer and without shaking Evaporation 300 mg ...

Page 47: ...ee 2 according to IEC 60664 1 see Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its means of overvoltage protection For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to...

Page 48: ...Generic emission standard Residential commercial and light industry EN 50082 1 1997 Generic immunity standard Residential commercial and light industry EN 61326 1 1997 A1 1998 Product family standard Test standards Performance limits EN 55022 1998 Class B 150 kHz 1 GHz EN 61000 3 2 1995 A1 1998 A2 1998 A13 1997 A14 2000 Class A EN 61000 3 3 1995 ANSI C63 4 1992 Class B 450 kHz 1 GHz EN 61000 4 2 1...

Page 49: ...icroplate holders 220 240 V 5112207 iEMS Incubator Shaker with nine 9 thermal microplate holders 100 120 V 5921200 iEMS thermal microplate holder 10 1 List of accessories and recommended spare parts Code Item 1610420 Dust cover for iEMS Incubator Shaker 3 modules 5180190 RS Device link cable 1506200A IEMS Incubator Shaker User Manual 1210070 Fuse 3 15 A 1210550 Power cord Euro 230 V 1210520 Power ...

Page 50: ...m an authorized Thermo distributor The guarantee is not transferable to a third party without prior written approval from Thermo This guarantee is subject to the following exclusions Any defects caused by normal wear and tear Defects caused by fire lightning flood earthquake explosion sabotage war riot or any other occurrence of the character listed above Refurbished products that are subject to d...

Page 51: ...ns 50 Contact information 58 Control LEDs 10 23 40 D Decontamination 37 Certificate of 56 instrument surface 38 procedure 37 thermal microplate holder 38 Dimensions 45 Directives 48 Disinfectants 37 Display 12 Disposal instrument 39 materials 37 E Environmental conditions 45 Evaporation 41 46 F Feedback Form 57 Fuse block assembly 16 Fuses 36 45 H Heating 31 Heating time 21 Holder 13 I Incubation ...

Page 52: ...keys 12 L LC display 12 M Mains supply cable 17 Mains switch 17 Maintenance 35 Microplate disposal of 37 43 placing of 24 Microplate holder 13 O Operating conditions 15 Operation normal 27 28 preheating and heating 31 principle 9 19 programming protocols 20 shaking 33 Ordering information 49 P Packing disposal of materials 39 for service 39 list 15 Parameters incubation temperature 20 incubation t...

Page 53: ...tup 18 Shaking 33 speed 26 40 42 time 21 Site of operation 15 Slot status 23 Slot temperature 42 Slot warmup time 42 Specifications 45 general 45 incubator 46 safety 47 shaker 46 Speed settings 22 Spills 35 41 Splashing 22 33 41 T Temperature setting 26 42 Thermal microplate holder 13 cleaning 35 placing of 24 Transport damage 15 Transport package 14 15 U Unpacking 14 V Viewing parameters 34 41 Vo...

Page 54: ...nt well formats e g 6 12 24 48 96 384 864 etc wells for ease of use in performing multiple tests through techniques such as EIA or ELISA Orbital shaking The shaking of a microplate in a circular movement with a fixed orbit and adjustable speed rpm Revolutions per minute 13 1 Keywords for web pages drug discovery Microtiter plate EIA microwell plate ELISA orbital shaker enzyme immunoassay orbital s...

Page 55: ...perature C Incubation time hh mm ss Shaking time hh mm ss Interval time hh mm ss Shaking speed as shown below Value 0 1 2 3 4 5 Shake speed rpm 0 400 650 900 1150 1400 5 Press temp followed by the slot number to start the preheating of the thermal microplate holder Refer to 6 5 6 Wait until the control LED changes from a flashing orange to a continuous orange 7 Press the slot number 1 9 and then s...

Page 56: ... any instrument s or item s ensure that they are fully decontaminated Confirm A or B status Name Address Tel Fax Name Serial no A I confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to hea...

Page 57: ...y Internet home page Date of purchase Your research area Dr Mr Mrs Ms Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above As Below Comments expectations expected expectations Reagent kit Instructions Instrument User manual Operational reliability Design Ease of use Operational costs Customer support Further instrument system developments desi...

Page 58: ...506200A 58 Appendix D Addresses For the latest information on products and services visit our worldwide web sites on the Internet at http www thermo com Manufactured by Distributed by P O Box 100 FIN 01621 Vantaa Finland Tel 358 9 329 100 Fax 358 9 3291 0415 www thermo com ...

Page 59: ...iEMS Incubator Shaker User Manual Rev 3 0 Cat no 1506200A 59 ...

Page 60: ...iEMS Incubator Shaker User Manual Rev 3 0 Cat no 1506200A 60 ...

Page 61: ...iEMS Incubator Shaker User Manual Rev 3 0 Cat no 1506200A 61 ...

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