Thermo Electron iEMS, User Manual

Page: 22 / 61

Chapter 6: Operation
iEMS

Incubator/Shaker User Manual Rev. 3.0, Cat. no. 1506200A 22
Note: The slots within a module shake at the speed that is programmed into
the most recently started processing protocol. Incorrect shaking speed
may result if you start a protocol within the same module that has a
shaking speed different from the other slots already in use. To minimize
the likelihood of this occurring, it is recommended that you program all
processing protocols within a module with the same shaking speed.
Caution: Do not place the microplate holder in or remove it from the instrument
while the instrument is shaking. This may damage the shaking
mechanism.
The shaking speeds (in revolutions per minute) corresponding to the values you
can enter are shown in the table below:
Table 6.1 Speed settings of the shaker
Value 0 1 2 3 4 5
Shake speed (rpm) 0 400 650 900 1150 1400
Caution: Splashing may occur if you use shaking with wells that contain too
much liquid for a given speed. To avoid splashing and potential cross
contamination from well to well, do not overfill wells for a given shaking
speed setting.
When the well is filled to 50% of its nominal volume, the shake speed
setting should not exceed value 4. It is recommended to use the setting
3 in most cases.
Program the shaking speed by
pressing the appropriate number key
corresponding to the required speed.
For example, for a speed of 900 rpm:
Press
3 ↵
(Enter)
If no shaking is desired, select the
value
0
. In this incubation module,
shaking is now disabled in every slot.
If you try to start a slot with a protocol
with shaking, an error message will
appear on the display:
On the display, the READY state will
appear after the programming phase.
SHAKE SPEED 0 rpm
VALUE 0 1 2 3 4 5
SHAKE SPEED 900 rpm
VALUE 0 1 2 3 4 5
PLACE 2
PROHIBITED OPERATION
READY 09:26:15