24 |
P a g e
G e n e r a l D a t a H e a l t h c a r e , I n c .
Problem
Description
Remedy
·The section is curved or damaged
(cont.)
·
The density of the paraffin is
uneven
·
Other outside factors (light,
heater or ventilation affect the
temperature around the
embedded specimen)
·
Remove the uneven paraffin
and embed the specimen again
·
Operate the microtome in a
temperature controlled
environment.
·
The section is extremely
compressed or wrinkled
·
Blade edge is dull.
·
Ambient temperature is too
high
·
The inclined angle of the blade
is too small
·
There is a build-up of paraffin
wax on blade edge.
·
The wax has been used several
times which cause the section
to be damaged
·
Sharpen the knife or change
the blade, or adjust the blade
position in horizontal
orientation
·
Cool down the embedded
specimen and blade in an ice
bath or embed the specimen
with the paraffin which is not
too soft and not too hard
·
Increase the angle to avoid the
incline plane rubbing the
embedded
specimen
·
Use the wet-cotton and the
cleansing liquid to clean two
sides of the blade from the
bottom. Never clean the blade
from the blade edge because it
may damage the blade and
result in personal injury
·
Change the paraffin, and
embed the specimen again
·
The section is broken or the
specimen is torn.
·
The tissue is not completely
dehydrated, or it is not cleaned
correctly.
·
If the specimen is too soft, it
means the specimen is not
completely penetrated with
paraffin.
·
The specimen is immersed in
the paraffin too long, or the
paraffin is too ho.t
·
The specimen is too hard.
·
Dehydrate or clean the tissue
again.
·
Penetrate the specimen with
paraffin and embed it again.
·
S
aving the specimen is
impossible because of damage
to the tissue.
·
Embed the specimen (tissue)
again in the plastic
（
histo
resin
）
or paraffin not too hard
and not too soft.
Summary of Contents for SHURCut 2500
Page 2: ......
