33
4.
MAINTENANCE
4.1
Cleaning
Routinely clean the exterior and the base plate of the unit by wiping them with a dry cloth to
remove dust and fine pieces of glass. Avoid contact with the filament.
Figure 4-1. V-Groove bearings and puller bars.
Occasionally the V-groove bearings (S in Figure 4-1) and the edges of the pull bars that slide
in their grooves (G in Figure 4-1) must be cleaned to maintain reproducibility from pull to
pull. This should be done using a dry cotton swab.
CAUTION: DO NOT lubricate any components of the P-97!
4.2
Heating Filament Replacement
NOTE: See Sutter Instrument’s YouTube video “Installing a Filament
(https://www.youtube.com/watch?v=cCsJsIZlzLw)
.
4.2.1
Filament Replacement (Step by Step)
1.
Remove the chamber that encloses the heating filament (remove the black thumbscrew at
the lower left corner of the Plexiglas cover plate and pull the chamber straight off).
2.
Loosen the two clamp screws (D’ in Figure 4-2, only one shown) that hold the filament in
place, and then slide out the old filament.
3.
Slip in a new filament, center it over the air jet, and then retighten the two clamp screws.
4.
Reinstall the chamber that encloses the heating filament and retighten the black
thumbscrew (lower left corner of the Plexiglass cover plate).
4.2.2
Air jet Position
The air jet should be from 2 to 3 mm below the center of the filament. If it not within this
specification, then loosen the screw holding the air jet in place and reposition it.
4.2.3
Positioning the Filament in Relation to the Glass Capillary
The correct position of the glass capillary in each of the two filament types is shown above in
the Heating Filament section. This positioning is critical to achieving the desired pipette’s tip
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)
Summary of Contents for P-97
Page 3: ......
Page 4: ......
Page 66: ...56 NOTES P 97 FLAMING BROWN MICROPIPETTE PULLER OPERATION MANUAL REV 2 43 DOM 20161118...
