13
steps of about 5 units since in most cases smaller changes will have little
effect.
PULL
(Range 0-255)
This parameter controls the force of the hard pull. In general, the higher
the pull, the smaller the pipette’s tip diameter and the longer its taper.
Useful changes in PULL strength are 10 units or more to see an effect.
NOTE: A one-line program containing PULL = 0 and a low VELOCITY
setting (15 – 40), looped 2-3 times, can be used for making short patch-
type pipettes.
VELOCITY
(Range 0-255)
The VELOCITY of the glass carriage system is measured as the glass
softens and begins to pull apart under a constant load. The increasing
velocity of the initial pull is determined by the viscosity of the glass,
which in turn is a function of the glass temperature. The adjustable
velocity allows for the selection of a precise glass viscosity as the trip
point for the hard pull. Useful values for velocity range from 10 to 150,
with lower values (15-40) being used for patch pipettes and higher values
(50-125) for microinjection pipettes. ..
VELOCITY = 0 Special Condition (Fire Polish Mode): If VEL=0 and
PULL=0, the HEAT will be on for the duration of the TIME programmed
(1 unit equals 10ms). This feature allows you to use the puller to fire
polish the resulting patch pipette...
TIME
(Range 0-255)
Controls the length of time the cooling air is active. This parameter is one
of two available modes of cooling If VEL>0 then one unit of TIME
represents 1/2ms. If VEL=0 (when in fire-polish mode), then one unit of
TIME represents 10ms.
TIME = 0 Special Condition: The air solenoid is disabled when TIME=0
(no active cooling). This allows the pulling of special pipette shapes. Most
often used to pull long tube-like shapes such as those used for
microperfusion.
DELAY
(Range 0-255)
A cooling mode that controls the delay time between when the HEAT
turns off and when the hard PULL is activated. The air is automatically
turned on for 300ms. The higher the DELAY value, the cooler the glass
will be when the hard PULL is executed. Thus, increasing the DELAY
results in decreased taper length and increased tip diameter. If VEL>0
then one unit of DELAY represents 1 ms. If VEL=0 then one unit
represents 10 ms.
DELAY = 0 Special Condition: The air solenoid is disabled when DELAY
= 0 (no active cooling). This allows the pulling of special pipette shapes.
Most often used to pull long tube-likes shapes such as those used for
aspiration, microperfusion, or holding.
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)
Summary of Contents for P-97
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