12
d.
Pull both bars towards each other using the finger holds. Hold the puller bars in
position using the thumb and index finger of one hand. The hex head screw should be
touching the end of the slot in both puller bars.
e.
Loosen both clamping knobs and carefully slide the glass through the holes in the side
of the heater chamber and into V-groove of opposite puller bar.
f.
Tighten down both clamping knobs.
7.
Press the < PULL > key on the keypad. The heating filament should turn on and the
glass should separate in less than 10 seconds.
8.
Loosen the clamping knobs and remove the pipettes from the puller bars.
Unless otherwise stated on your program sheet, Program 0 is factory pre-programmed to pull
a micropipette (tip diameter less than 0.1
μ
m) from 1.0mm x 0.5mm borosilicate glass.
Program 1 is factory pre-programmed to pull a patch type pipette using 1 x 0.5 mm glass to
make a tip diameter of about 1
μ
m. To try the patch pipette program (#1):
1.
Press RESET to exit Program 0
2.
Press < 1 > followed by < ENTR > to enter into Program 1.
3.
Repeat Steps 4-7 above.
3.2
Programs
3.2.1
Program Structure
The resulting size and shape of a micropipette made using the P-97 is determined by the
parameter values that are programmed by the user. One hundred (100) separate programs
can be saved for future use. Each program is structured as follows:
Program
Consists of one or more Lines, each of which represents a pull cycle.
When a program is run, all lines within the program are sequentially
executed, beginning with Line 1. As a line is executed, the capillary glass
inserted in the instrument is “pulled.” A program can be up to 8
lines/cycles in length.
Cycle
A Cycle consists of five programmable parameters; Heat, Pull, Velocity,
either Time or Delay, and Pressure. A Cycle is equivalent to one line of
Program code.
3.2.2
Program Line Pull Cycle Parameters
Each of the four programmable parameters in a Cycle is defined below:
HEAT
HEAT controls the level of electrical current supplied to the filament. The
HEAT required to melt a piece of glass is a function of the filament
installed and the particular glass size and composition. It is important
that the HEAT value be set relative to the Ramp Test value as discussed
in the Operation Section. Generally, changes to HEAT will be made in
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)
Summary of Contents for P-97
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