14
3.2.3
Pull Cycle
A typical pull cycle in a program line is described below:
1.
The heat turns on.
1.
The filament heats up, the glass softens, and a weak pull draws the glass out until it
reaches the programmed velocity.
2.
When the programmed velocity has been reached, the heat turns off, and the air turns on
to cool the filament and glass.
3.
If TIME is greater than 0 (zero), the hard pull (if any) is executed after a short delay and
then the air is activated for the specified TIME.
If DELAY is greater than 0 (zero), the air is activated for a short period and then the hard
pull is activated after the specified DELAY.
3.2.4
Default Configuration
Unless requesting special programming or setup at the time of purchase, the puller is setup
and shipped with the following standard factory configuration:
Table 3-1. Standard factory configuration.
Heating Filament Installed
2.5mm Box (Catalog # FB255B)
Glass used to program puller
(sample sent with puller)
1.0mm O.D. x 0.5mm I.D. borosilicate,
with filament (Catalog # BF100-50-10)
Factory installed programs
(see enclosed program sheet)
0 -Micropipette (tip diameter less than 0.1
μ
m
1 -Patch pipette
In describing the operation of the puller, the above configuration is assumed. If the
configuration of your puller differs, the operating instructions still apply but references to
specific program settings may not be accurate. Inappropriate settings will generally only
affect your ability to control the geometry of the glass micropipette you are trying to
fabricate. However, the heating filament can be destroyed by an excessive value for the
HEAT parameter. We recommend you refrain from executing a program until you have read
this section of the manual and have run the Ramp Test described herein.
3.2.5
Selecting a Program (0 to 99)
After applying power to the instrument, a “power on” reset will occur and the display will
appear as follows:
COPYRIGHT SUTTER INSTRUMENT CORP. 1992
WHICH PROGRAM DO YOU WANT? (0-99)
Figure 3-2. Power-on display.
The P-97 has the capacity to store 100 programs (0 - 99). On the keypad, press < 0 >
followed by < ENTR > (factory installed micropipette program) or the number of another
program you wish to execute. The display will appear as shown below in Figure 3-3
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)
Summary of Contents for P-97
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