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· ErgoOne · User Manual
Implementation
a)
Adjust the pipette to the required
nominal volume.
b)
Carry out ten pipettings with sub-
sequent weighing and record the
individual measurements.
c)
Calculate the mean value:
Mean value x =
xi = weighing results
n = number of individual weighings
d)
Calculate the corresponding volume:
Volume (µl) =
x = calculated mean value
The calculated volume corresponds
to the actual setting of your pipette
(the density of water at 20°C =
0.9982 g/ml).
e)
If realignment is necessary, open the
volume adjustment catch by pulling
up the volume control button
1
until
you hear a click. Remove the cover
of the alignment unit
3
. Slide the
button down, using a pointed object
or pen (with the nib retracted), and
(whilst holding the button down)
turn the volume control button
1
to change the piston stroke of the
pipette (the volume display remains
unchanged). Turn the volume control
∑ xi
n
7. Maintenance
ErgoOne
®
is a low maintenance pipette. However, individual working methods,
length of application and internal quality control guidelines mean that regular
monitoring and maintenance of your pipette is necessary.
Note: If you make any changes to the pipette or replace individual parts, a check with
recalibration is required. If the pipette cannot be calibrated successfully or if a fault
continues, contact STARLAB.
Volume change
ErgoOne
®
Single-Channel
Volume change
approx.
0.1 – 2.5µl
0.13µl
0.5 – 10µl
0.7µl
2 – 20µl (micro cone)
1.4µl
2 – 20µl (standard cone)
1.4µl
10 – 100µl
7µl
20 – 200µl
14µl
100 – 1000µl
70µl
500 – 5000µl
340µl
ErgoOne
®
Multi-Channel
Volume change
approx.
0.5 – 10µl
0.7µl
10 – 100µl
7µl
30 – 300µl
14µl
Note: Multi-channel pipettes are only
aligned on one channel. Attach a single
tip to any channel and proceed as de-
scribed above.
button
1
clockwise to increase the
volume, counter clockwise to reduce
the volume (see table 2 for details).
Once alignment is complete, push
the volume control button
1
down
again until you hear a click. Replace
the alignment unit
3
cover, and the
pipette is ready for use again.
A full rotation of the volume control
button
1
corresponds to the following
volume change:
Table 2
x (mg)
Density of liquid
