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Operating Manual

Spex NanoSNAP™

Nano Spectrophotometer for Nucleic Acids

and Proteins Measurement

Summary of Contents for NanoSNAP

Page 1: ...Operating Manual Spex NanoSNAP Nano Spectrophotometer for Nucleic Acids and Proteins Measurement...

Page 2: ...tor 12 3 6 Basic Operation 12 4 0 Application Nucleic Acid 14 4 1 Overview of Screen Features 14 4 2 Protocol Operation 15 4 3 Calculation 16 5 0 Application Protein A280 17 5 1 Overview of Screen Fea...

Page 3: ...ser Folder 40 11 2 Viewing a User Folder 40 11 3 Editing a User Folder 40 11 4 Deleting a User Folder 40 11 5 Using a USB Flash Drive as a User Folder 41 12 0 Maintenance 41 12 1 Cleaning the Unit 41...

Page 4: ...on and Storage This instrument should be transported and stored in an environment with a temperature of 10 C to 60 C relative humidity 20 to 80 1 2 2 Installation and Operation 1 Indoor use only 2 Do...

Page 5: ...electrical installation and discard the others Mains Cord Replacement Should one of the power cords not be suitable for connecting to the power supply replace the plug with a suitable alternative THI...

Page 6: ...e carried out by authorized service personnel only 1 2 5 Instructions for Removal from Use Transportation or Disposal Do not dispose of this product as unsorted municipal waste Follow local municipal...

Page 7: ...easuring time at 3 seconds Wide measuring range from 190 to 1 000 nm Quartz sample window which protects the optical analysis system A nano hydrophobic coating layer on the quartz window Replaceable s...

Page 8: ...e Window Quartz glass with nano hydrophobic coating layer It has an indicating sample adding design It protects optical fibers and also reduces contamination Pathlength Selector Light path of Spex Nan...

Page 9: ...and other important information 3 0 Getting Started 3 1 Unpacking Once you open the Spex NanoSNAP package confirm that all of the following items are included Spex NanoSNAP spectrophotometer Quick Sta...

Page 10: ...t in use Note The adapter is foolproof which requires more focus to plug in and out 3 3 Main Screen On the Main Screen there are some informational items indicating the status of the Spex NanoSNAP it...

Page 11: ...to the end as shown in Figure 5 Figure 5 Opening the arm To close the detection arm hold the edge of the arm and let the detection arm down gently to the correct position as shown in Figure 6 With th...

Page 12: ...hlength Hold the handle of the light path selector to adjust between 0 5 mm and 0 05 mm pathlength Table 5 Manual pathlength selector guide Position Pathlength Selector Measuring Range Figure 7 0 5 mm...

Page 13: ...hoose the correct Method type according to experiment 2 Ensure the surface of the sample window and the cover window are both clean 3 Adjust the pathlength selector to appropriate position light path...

Page 14: ...formation Features Description conc The concentration is calculated from absorbance at 260 nm and the unit is ng L A260 Displays the absorbance at 260 nm which is normalized to a 10 mm pathlength equi...

Page 15: ...re the Blank again if the original blank data has been renamed Note Only one data item can be checked at one time 4 1 3 Graph Tab Page The graph of measuring samples can be enlarged or minimized The a...

Page 16: ...efault of the Auto Run function is off If Auto Run is turned on sample measurement will be performed automatically after closing the detection arm Note In Nucleic Acid dsDNA RNA and ssDNA applications...

Page 17: ...ow Table 10 Figure 12 Data Tab Page Table 10 Data tab page information Features Description conc The concentration is calculated from absorbance at 280 nm and the unit is mg mL A280 Displays the absor...

Page 18: ...ure the Blank again if the original blank data has been renamed Note Only one data item can be checked at one time 5 1 3 Graph Tab Page The graph of measuring samples can be enlarged or minimized The...

Page 19: ...lly after closing the detection arm Note 1 In Protein A280 BSA lgG Lysosome 1 A 1 mg mL and customized protein factor applications are offered in this protocol If you need to test other samples please...

Page 20: ...escription conc The concentration is calculated from absorbance at kit requested wavelength The unit is mg mL A562 A595 A750 Displays the absorbance at kit requested wavelength The BCA method uses 562...

Page 21: ...calculated automatically Curve Type The standard curve types the user can select linear interpolation and 2nd order polynomial Repetition The repetition frequency of standard numbers The default valu...

Page 22: ...on 1 On the main menu tap on the Protein Assay icon to enter the protocol section 2 Ensure the sample window and the cover window on the detection arm are clean 3 Select the correct Method according t...

Page 23: ...he Measure icon to establish standard data If the repetition frequency is higher than 1 it will automatically jump to the next cell 10 If you want to correct the standard absorbance select the Measure...

Page 24: ...can uncheck Generate Std Curve and continue to insert data 6 3 Calculation For protein quantification the concentration can be calculated by measuring the final absorbance of colorimetric samples and...

Page 25: ...col Figure 19 the data information parts have the features below Table 14 Figure 19 Data Tab Page Table 14 Data tab page information Features Description conc The concentration is calculated from abso...

Page 26: ...ain if the original blank data has been renamed Note Only one data item can be checked at one time 7 1 3 Graph Tab Page The graph of measuring samples can be enlarged or minimized The axis can be move...

Page 27: ...time 11 The default of the Auto Run function is off If Auto Run is turned on sample measurement will be performed automatically after closing the detection arm Note 1 The conversion factor range is f...

Page 28: ...are offered for users Table 15 The function icons will be activated after inserting the Analysis Wavelength Units Conversion Factor optional and Correction Wavelength optional fields Table 15 Custom s...

Page 29: ...ned wavelength which is normalized to a 10 mm pathlength equivalent Anlaysis User defined wavelength decided on the custom setting page Corr Self defined wavelength for bichromatic normalization This...

Page 30: ...or if the user needs to convert absorbance to concentration optional 6 Insert the Correction Wavelength if the user needs bichromatic normalization optional 7 Add appropriate solution of at least 1 L...

Page 31: ...n optional 8 2 1 Overview In standard curve method the information tab bar has 5 parts custom settings tab page sample data tab page standard tab page table tab page and graph tab page The Custom Sett...

Page 32: ...Anlaysis User defined wavelength decided on the custom setting page Corr Self defined wavelength for bichromatic normalization This is an optional function and the default is Off Name The sample name...

Page 33: ...ck the icon to establish the standard curve The table tab page Figure 29 will show all the data results If the user needs to know more details of one data item tap the check box column to select the d...

Page 34: ...the repetition frequency you need in the Repetition bar 10 Tap on a cell in the conc column to enter the concentration of your standard sample 11 Tap on a cell in the Abs column and select the Measur...

Page 35: ...ffered 8 3 1 Overview The information tab bar has 4 parts custom setting tab page data tab page table tab page and graph tab page In the Custom Setting page Figure 31 there are 8 self defined waveleng...

Page 36: ...data results If the user needs to know more details of one data item tap the check box column to select the data The detail will be shown on the Data page and the Graph page Figure 33 Table Tab Page T...

Page 37: ...tion off the sample window and the cover window 6 Add your sample of at least 1 L and select the Measure icon to enter sample measurement 7 After the experiment clean the sample window and the cover w...

Page 38: ...rsion Initialization Date and Calibration Date The link to the Operating Manual is also here Brightness User can adjust the brightness of the display panel according to the environment s lighting cond...

Page 39: ...new protocol or view the report 10 1 Duplicating Report Setting Select the saved report with the protocol setting you want to duplicate Tap on the Plus icon to duplicate the report setting The new pr...

Page 40: ...then tap for a second time or tap on the Open icon to open it If the folder is password protected you ll be requested to input the password Input the password and tap on OK to confirm the password or...

Page 41: ...e detection arm and wipe with a lint free wipe 70 ethanol or isopropanol can also be used on the surface to clean both the sample window and the cover window It is better to clean the quartz glass eve...

Page 42: ...for service Mechanism alignment problem Return the unit for service Detection time is too long Faulty electronic module Return the unit for service Faulty optics module Return the unit for service Pa...

Page 43: ...ice Er03 ERR_METER_NO_ANSWER Optics module problem Reboot the unit Er04 ERR_METER_CALIBRATE Optics module problem Reboot the unit Er05 ERR_UART_NO_ANSWER Electronic module board problem Reboot the uni...

Page 44: ...on Range dsDNA 2 to 20 000 ng L BSA 0 06 to 600 mg mL Sample Surface Material of Construction Lower and Upper Stainless steel and quartz window with hydrophobic treatment Measurement Time 3 seconds So...

Page 45: ...exsales antylia com 4864M Connect with us Operating Manual Appendix B Ordering Information Equipment Product Name Part Number Spex NanoSNAP UV Visible Microvolume Spectrophotometer 83057 02 Maintenanc...

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