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Sea-Bird Scientific SUNA V2, User Manual

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Sea-Bird Scientific SUNA V2 User Manual Download Page 41

Figure 5  Copper anti-fouling guard

7.6.2 Flow cell

Use the flow cell for moored deployments that require a pump. Attach the flow cell to the
sensor with the manufacturer-supplied plastic clamp. The flow cell seals against the
pressure housing of the sensor so that the water from the pump flows across the optical
path of the sensor. The flow cell has a copper tube on the inlet port and a plastic barbed
fitting on the outlet port that connects to the pump with flexible tubing. Spare parts for the
flow cell include elbow fittings so that the user can adjust the position of the sensor for
deployment.

Figure 6  SUNA flow cell

Reference

39

Summary of Contents for SUNA V2

Page 1: ...User manual SUNA V2 2k Submersible Ultraviolet Nitrate Analyzer Document No SUNASUNADeep Release Date 2022 05 24 Version G 425 643 9866 seabird com ...

Page 2: ......

Page 3: ...data 15 5 1 Export data to csv or MS Excel format 15 5 2 Options to process data 16 5 2 1 Convert SUNA raw data 16 5 2 2 Show SUNA data 16 5 2 3 Reprocess SUNA data 16 5 2 3 1 Nitrate reprocessing details 17 5 3 Show data from multiple sensors 19 Section 6 Maintenance 21 6 1 Sensor maintenance 21 6 2 Clean bulkhead connectors 21 6 3 Update reference spectrum 22 6 4 Compare reference spectrum files...

Page 4: ...Flow cell 39 Section 8 Software reference 41 8 1 Sensor dashboards 41 8 2 UCI menu 41 8 2 1 General tab 41 8 2 2 Display tab 41 8 2 3 Message tab 42 8 3 Sensor menu 43 8 3 1 SUNA 43 8 4 Data menu 43 8 4 1 Show data from multiple sensors 43 8 4 2 Export data to csv or MS Excel format 43 8 4 3 Show data from the sensor 44 8 5 View menu 44 8 6 Window and Help menus 44 8 7 Other general settings 45 Se...

Page 5: ...the sensor c Push Connect to start communication with the sensor d Push Start to collect data e Remove and replace the protective cover on the optical face several times to change the value of the collected data f Push Stop to stop data collection 3 Verify that the sensor transmits data Verify sensor transmits data on page 10 Push Transmit Data in the dashboard and select All Data to transmit data...

Page 6: ...SUNA quick start guide 4 ...

Page 7: ...wer 4 5 RS232 TX USB D 6 RS232 RX USB D 7 Analog V out 8 Analog current out 2 2 Electrical Input 8 18 VDC Current draw maximum 1 5 A Current draw operation 625 mA at 12 V nominal Current draw supervised low power periodic mode 30 µA Current draw processor low power controlled mode 3 mA Current draw standby 20 mA at 12 V Baud rate 57600 9600 19200 38400 115200 available Communication interface RS23...

Page 8: ...0 µM 42 mgN L 30 20 20 up to 4000 µM 56 mgN L N A N A N A The specified accuracy is best accuracy or a percentage whichever is more A sensor specific calibration uses extinction coefficients from the sensor itself A class based calibration uses extinction coefficients that are the average of many sensors 2 4 2 Nitrate measurement precision Processing configuration Seawater or fresh water with T S ...

Page 9: ...Verify sensor operation WA RNING Sensors that use ultraviolet light sources 400 nm Do not look directly at a UV light source when it is on It can cause damage to the eyes Keep products that have UV light sources away from children pets and other living organisms Wear polycarbonate UV resistant safety glasses to protect the eyes when a UV light is on 1 Connect the USB or RS232 cable to the PC For R...

Page 10: ...llect data Data Collection Push Start to save the collected data to the PC Time Series Spectra and Total Absorbance graphs 3 Put a check in the box next to any additional parameters so that they will show in the Time Series graph 4 Look at the data in the Time Series graph The user can look at data in real time for each sensor that has power supplied is connected and is in communication with the s...

Page 11: ... Series graph 5 Let the sensor or sensors collect data for approximately 5 minutes 3 4 1 Monitor data in spectra graph The Spectra graph shows both the dark and light data in raw counts The dark counts are from thermal noise The light counts are the measured output minus the dark counts The measured spectrum is always flat below 200 nm and then has four or five peaks The peaks are approximately 25...

Page 12: ...is an alternative to the Spectra graph The absorbance graph should be flat when a sample of DI water is collected The absorbance increases as absorbing species such as nitrate and bromide are added to samples If necessary push Stop 3 5 Verify sensor transmits data NOTIC E Use only the batteries recommended by the manufacturer as replacements Do not mix chemistries V Ah or new and used batteries If...

Page 13: ...t the user use a USB connection to move the files because it is much faster 3 Push the arrow to start the move The status shows at the bottom of the File Manager window 4 Open the file on the PC to make sure it has all of the collected data Set up sensor and verify operation 11 ...

Page 14: ...Set up sensor and verify operation 12 ...

Page 15: ...the box at Wiper Settings to enable the wiper f Select the Level INFO is the default Refer to the Message tab on page 42 for details about the levels g Push Next 3 Select the Data Settings a Select the Transmitted Frame Format FULL_ASCII is the default b Select the File Creation Method By File Size is the default Refer to Communication on page 27 for details The manufacturer recommends that the us...

Page 16: ...Deployment and recovery 14 ...

Page 17: ...the baud rate to the default for the connected sensor 4 In the Output CSV Data File area type a new file name or use the automatically generated file name 5 Optional change the time stamp to UTC 6 Push Transmit The data is copied to the PC The software uploads a raw sbsdat file and automatically converts the data to a readable csv file type The Transmit Progress window shows the status of the file...

Page 18: ...r Windows this is C Users USERNAME My Documents Sea Bird Scientific SUNA For Mac OS X this is Users Home Sea Bird Scientific SUNA The raw data file to process The calibration file for the sensor 1 Push Show Data from Sensor 2 If necessary push Browse to find the xml Instrument Package File that is saved on the PC A typical file is SUNA_xxx_001 xml where xxx is the serial number of the sensor 3 Pus...

Page 19: ...rate Data Files will show in the dashboard and the user can select some or all to process Spectral data which is recorded in full ASCII full and reduced binary and APF frames is required to process nitrate data Use the reduced binary and APF frames to reprocess data from seawater deployments Note that the APF frames only let the user reprocess data that was collected with the integration time adju...

Page 20: ...at is measured To mitigate this effect use the sample temperature and salinity values in the nitrate calculation in the host software for post processing Note that spectra and related temperature and salinity data is necessary The temperature salinity correction comes from MBARI cf Carole M Sakamoto Kenneth S Johnson and Luke Coletti Limnol Oceanogr Methods 7 2009 Temperature and Salinity Correcti...

Page 21: ... The lower area shows a timeline of the original and reprocessed nitrate concentrations Use the slider under the timeline to select one measurement and see the concentration to the left 5 3 Show data from multiple sensors The software lets the user show data that has been saved on the PC from each supported sensor The user can select up to 12 parameters to look at 1 Go to the Data menu then Show D...

Page 22: ...Transmit data 20 ...

Page 23: ...ir 5 Put the sensor in the manufacturer supplied case for transport or storage 6 2 Clean bulkhead connectors NOTIC E Do not use WD 40 or petroleum based lubricants on bulkhead connectors It will cause damage to the rubber Damaged connectors can cause a loss of data and additional costs for service Damaged connectors can cause damage to the sensor and make it unserviceable Examine clean and lubrica...

Page 24: ...erence spectrum of the SUNA at regular intervals so that the sensor collects accurate data It may also be necessary to update the firmware although that is not required very frequently A calibration file contains the data required to convert a spectral measurement into a nitrate concentration The calibration data are the wavelengths of the spectrum the extinction coefficients of chemical species a...

Page 25: ...lush the sensor and the optical area with clean water to remove debris and saltwater b Clean the metal parts external to the optical area so that the Parafilm will seal c Std SUNA only If the sensor is equipped with a wiper carefully move it away from the optical area Figure 1 Parafilm to be wound around optical area 2 Cut and stretch a length of approximately 40 cm 16 in of Parafilm 3 Wind severa...

Page 26: ...2 Flush the optical area with DI water to remove any remaining IPA or vinegar 13 Wind the Parafilm around the metal near the optical area 14 Break a small hole in the top of the Parafilm and fill the optical area with fresh DI water 15 Supply power to the sensor and start the software if necessary 16 Push Reference Update The Reference Update Wizard shows 17 Fill the sample volume with distilled w...

Page 27: ...or 4 Push Compare A typical update interval of 3 6 months with no more than 100 hours of lamp use should cause a change of no more than 10 in the 215 240 nm interval Below 215 nm larger relative changes are normal Above 240 nm the change is smaller than at the 215 240 nm range If there is a large change do several reference updates 12 24 hours apart to monitor the stability of the reference spectr...

Page 28: ... Push Browse to find the firmware file that is saved on the PC 7 Push Open 8 Push Upload It takes approximately 2 minutes for the software to be updated The software disconnects the sensor Maintenance 26 ...

Page 29: ...ata output This increases the rate at which data is collected and uses 10 30 less power The user can set the Frame Format to NONE to turn off internal data collection The default File Creation Method is By File Size Others are available By File Size The software makes a new file when the data file in use gets to the user selected maximum size The file name starts with C Continuous file By Sample E...

Page 30: ...m channels 256 x AI 256 x BU 2 269 Internal temperature C AF BF 4 270 Spectrometer temperature C AF BF 4 271 Lamp temperature C AF BF 4 272 Cumulative lamp on time secs AI BU 4 273 Relative humidity AF BF 4 274 Main voltage V AF BF 4 275 Lamp voltage V AF BF 4 276 Internal voltage V AF BF 4 277 Main current mA AF BF 4 278 Fit aux 1 AF BF 4 279 Fit aux 2 AF BF 4 280 Fit base 1 AF BF 4 281 Fit base ...

Page 31: ...collect a dark spectrum measurement on a time based or measurement based schedule A typical setup is a 1 second dark spectrum measurement for each 20 30 seconds 20 30 measurements of the light spectrum 7 1 3 Logger controlled operation Use the software to see and change the settings for this mode of operation Use the commands below to communicate with the sensor in RS232 Command Result Start Start...

Page 32: ...e the settings for this mode of operation 1 Select a Sample Interval from the drop down menu 2 Look at the Offset value An offset value of 300 5 min changes the start time by five minutes for example from 06 00 to 06 05 3 The Light Frames value is 0 255 Data is collected for either a user selected quantity of time or a user selected number of measurements Note Periodic and Autonomous are both term...

Page 33: ...ion function is available if the sensor has analog output The sensor gives voltage and current output of the calculated nitrate values A 12 bit digital to analog converter DAC is used to give a voltage range of 0 095 4 095 A 16 bit DAC gives a current range of 4 20 mA The sensor has two configuration values DAC minimum nitrate and DAC maximum nitrate that are the lowest and highest values for volt...

Page 34: ...n A1 Vlow is the in system calibrated voltage offset coefficient B1 DACmax DACmin Ihigh Ilow is the in system calibrated current scale coefficient B0 DACmin B1 Ilow is the in system calibrated current offset coefficient 7 2 Terminal program The user can communicate with the sensor through the manufacturer supplied software or by use of a terminal program through the serial port on a PC Examples of...

Page 35: ...ation get config The sensor shows the current setup configuration Get mode of operation get opermode The sensor shows the current mode of operation Set mode of operation set opermode Changes the mode of operation 7 To change the mode of operation to SDI 12 a Make sure that the sensor is so equipped Type getopermodesdi12brd The response is available If the response is missing the sensor is not equi...

Page 36: ... 1 65535 10 lgtsmpls Dark duration sec 1 65535 10 drkdurat Light duration sec 1 65535 120 lgtdurat External device none wiper none exdevtyp Ext dev pre run time sec 1 120 0 exdevper Ext dev during collection on off off exdevrun Ext dev minimum interval 1 1440 60 exdvival Periodic Mode of Operation When power is supplied to the sensor the sensor goes to a low power standby mode Any activity on the ...

Page 37: ...time that is 20 times as long as the normal integration time This increases the signal to noise ratio in faint light conditions and lets the sensor operate in optically dense conditions When the optical density decreases the sensor goes back to the normal spectrometer integration time 7 3 2 Description of nutrient units Nutrient units express the amount of something usually moles or mass relative ...

Page 38: ... that the sensor collects accurate data because the optical components change over time For best performance send the sensor back to the manufacturer annually for maintenance 7 4 Interferences and mitigation 7 4 1 Uncharacterized species in sample A number of substances in natural water absorb in the UV spectral range where nitrate absorbs The spectral signature is usually different from nitrate b...

Page 39: ...pact analysis 7 4 4 Sensor function The lamp and other optical components in the sensor change with time This causes nitrate measurements to change or drift Do regular updates to the reference or baseline spectrum to minimize this change The output of the lamp is related to its temperature The manufacturer recommends that the user collect the reference baseline spectrum in conditions that are almo...

Page 40: ...proxy if the composition is stable over time Most FDOM sensors use a 370 nm excitation source that targets more humic substances and may not represent absorption in the nitrate range 220 nm Absorption The data output of the SUNA V2 is the absorption at 350 nm and 254 nm A350 and A254 These wavelengths are outside the nitrate absorption range and can be used to make an estimate of the impact of CDO...

Page 41: ...st the pressure housing of the sensor so that the water from the pump flows across the optical path of the sensor The flow cell has a copper tube on the inlet port and a plastic barbed fitting on the outlet port that connects to the pump with flexible tubing Spare parts for the flow cell include elbow fittings so that the user can adjust the position of the sensor for deployment Figure 6 SUNA flow...

Page 42: ...Reference 40 ...

Page 43: ... Terminal lets the user send terminal program level commands to the sensor 8 2 UCI menu The user can set up the way that the software saves information about the operation of the sensor and change the way that the data looks from the Preferences menu 8 2 1 General tab Enter or change the directory on the PC in which data from the sensor is stored Maximum Wakeup Attempts lets the user select the nu...

Page 44: ...s that have information about sensor use data collection and software operation over time This information helps the user and Customer Support find problems and troubleshoot When the Display Dialog for Error Level Messages box has a check in it the software saves one of five levels of error messages INFO The default level All high level operations are saved ERROR Minimum level Only errors that nee...

Page 45: ...4 Data menu The Data menu has sensor specific options to look at data that has been saved to a PC 8 4 1 Show data from multiple sensors The software lets the user show data that has been saved on the PC from each supported sensor The user can select up to 12 parameters to look at 1 Go to the Data menu then Show Data from Sensor 2 Go to the area of the first supported sensor and push Browse to go t...

Page 46: ... push Configure to change the number of graphs to view The Graph History Limit range is 1 2147483647 Put a check in the Graph History Unbounded box to see the total number of graphs Default value no check in the box and a Graph History Limit of 1 SUNA Reprocessed Nitrate graph the user may find that it helps to reprocess data under some conditions Output Go to the View menu and select Output then ...

Page 47: ...eb site 8 7 Other general settings Sample Averaging Deployment Characteristics The average of the number of frames selected The higher the value the more time it takes to make a measurement The Estimated Frame Rate is directly related to the Number of Measurements per Frame The manufacturer recommends a value of 10 15 as the Number of Measurements per Frame to increase precision in a stable water ...

Page 48: ...Software reference 46 ...

Page 49: ...pot If there is not the sensor may be in an unknown mode or there is a system failure current 5 mA sensor is in standby mode and gives a result over the RS232 input current 100 µA sensor is not in operation Contact Customer Support Table 5 Operations Possible problem Possible solution Self test fails for internal humidity Monitor the sensor and do a sensor self test at regular intervals Make sure ...

Page 50: ...ed in Fresh Water setting has a check in the box if the sensor operates in fresh water Poor correlation with field samples grab samples Look at the sensor for fouling or lamp change drift Clean the optical windows and do a blank test Set the sensor to do a self test Check light dark counts such as high CDOM or other absorbers and other data about the sensor Look for matrix interferences that can c...

Page 51: ...t 2 Remove all batteries from the sensor if so equipped 3 Remove all anti fouling treatments and devices Note The manufacturer will not accept sensors that have been treated with anti fouling compounds for service or repair This includes AF 24173 devices tri butyl tin marine anti fouling paint ablative coatings etc 4 Use the sensor s original ruggedized shipping case to send the sensor back to the...

Page 52: ...General information 50 ...

Page 53: ......

Page 54: ...Sea Bird Electronics 13431 NE 20th Street Bellevue WA 98005 U S A 425 643 9866 Sea Bird Electronics Inc 2022 All rights reserved SUNASUNADeep ...

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