r3bEL mini User Manual
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6
Setting up for Printing
6.1
Loading and Unloading Syringes
Before loading or unloading syringes, ensure that the r3bEL mini bioprinter is connected to your
computer via USB and open the Pronterface program.
6.1.1
Loading a syringe
1.
Insert the barrel flanges into the barrel flange slot and try to align the syringe plunger to fit
in the slot in the extruder (see figure below). If the extruder slot does not align with the
syringe plunger move the extruder higher or lower by clicking on “extrude” or “retract”
accordingly until the slot in the extruder aligns with the syringe plunger. Then slowly push
the syringe in until it locks into position on the metal holder.
2.
After the syringe is in place extrude in 5 or 1 mm increments until liquid is pushed out of
the needle tip. This is referred to as “priming” the syringe. Wipe off any excess liquid before
printing.
6.1.2
Unloading a syringe
1.
Retract the extruder by 5 mm or 1 mm to make sure that the syringe plunger is loose and
not in contact with the top of the extruder.
2.
Pull the syringe slowly and you should be able to get the syringe out smoothly.