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r3bEL mini User Manual 

 

3 

 

 

9.3

 

Cleaning the printer ......................................................................................... 45

 

10

 

Advanced Settings ............................................................................................ 45

 

10.1 G-codeAdjustments ........................................................................................ 45

 

10.2 G-code Customization .................................................................................... 46

 

11

 

Troubleshooting guide ....................................................................................... 48

 

12

 

Technical support .............................................................................................. 50

 

13

 

Specifications .................................................................................................... 51

 

 

 

 

 

Summary of Contents for r3bEL mini

Page 1: ...User Guide Version 1 0 ...

Page 2: ...1 4 2 Setting up Pronterface 18 4 3 Custom Buttons 20 4 4 Understanding the Custom Button Functions 22 5 Connect via Wi Fi 23 5 1 Printer Driver Installation Windows 23 5 2 Configuring the Wi Fi 26 5 2 1 YAT Terminal Windows 26 5 2 2 Coolterm macOS 31 5 3 Web Control Interface 37 6 Setting up for Printing 38 6 1 Loading and Unloading Syringes 38 6 1 1 Loading a syringe 38 6 1 2 Unloading a syringe...

Page 3: ...3bEL mini User Manual 3 9 3 Cleaning the printer 45 10 Advanced Settings 45 10 1 G codeAdjustments 45 10 2 G code Customization 46 11 Troubleshooting guide 48 12 Technical support 50 13 Specifications 51 ...

Page 4: ...e of Product The limited warranty shall be voided if alleged Product failure was caused in whole or part by i improperly or incorrectly performed modifications alterations or repair ii parts or consumables were installed and used that were not certified or approved by SE3D or its designated representative iii unusual physical or electrical stress iv abuse misuse accident neglect v exposure to exce...

Page 5: ...r3bEL mini User Manual 5 1 r3bEL mini Overview Printer axis ...

Page 6: ...s especially with sharp needle tips extra care should be taken to avoid poking or stabbing yourself Use only blunt tip syringe needles for all bioprinting activities For disposal and handling of biohazardous waste or contaminated items please reference and follow the safety guidelines for the laboratory 2 2 Protection The r3bEL bioprinter should not be exposed to excessive liquid or damage may occ...

Page 7: ...ed template 2 Power supply cable 3 Accessory bag 5 mL Syringe 22 Gauge Needle Micro USB Cable and SD card reader 4 Allen wrench Be sure to remove all packaging from the bioprinter before use 3 1 Additional Items You will be provided with a 5 mL syringe and 22 gauge blunt tip needle to use with your bioprinter for initial testing You can purchase additional consumables at our website se3d com ...

Page 8: ...nter control software that controls and operates a 3D printer This software will be used to control the r3bEL mini bioprinter via USB Follow these instructions to download the software 1 Go to portal se3d com and click on Log In in the top right corner and register 2 Once you have registered you will get a confirmation email with a link to activate your account After you activate your account you ...

Page 9: ... to the printer so that the correct device drivers are installed For installation of Arduino IDE you must go directly to their website 1 Go to https www arduino cc en Main Software 2 Choose your operating system from the box shown below 3 Click Just download from the contribution page unless you want to contribute ...

Page 10: ...select a folder destination and click Extract b Find the file in your Download folder Open the zip file Drag and drop the folder called arduino 1 to your Desktop 5 The next step is to install the drivers Go to Tools Boards Boards Manager a In the Boards Manager pop up widow type Arduino Due in the search box and select the result titled Arduino SAM Boards 32 bits ARM Cortex M3 and click Install as...

Page 11: ...t from those used for regular 3D printing You must load the config file provided by SE3D when using Slic3r Follow the instructions below for installation of program and config files You should find the Slic3r program in the Programs to Download zip file when you downloaded Pronterface earlier To download the Config files for Slic3r settings go to Startup guide r3bEL mini and click on the Config fi...

Page 12: ...nual 12 1 You will first be prompted with the Welcome screen Click Next 2 For G code flavor select RepRap Marlin Sprinter Repetier 3 For Bed Size use the default x 120 and y 130 setting since our print bed is 120 x 130mm ...

Page 13: ...et the nozzle diameter to 0 5 mm 5 Set the filament diameter to 3 mm 6 Set extrusion temperature to 0 C since there is no heating element on the r3bEL mini bioprinter tool head 7 Set bed temperature to 0 and click next Then finish ...

Page 14: ...izard the Slic3r program should look like this Now to load the lotion config file by following these steps Note we use lotion as the mock biomaterial to perform baseline tool calibration 1 Go to File Preferences for Windows users For macOS users to go Slicer Preferences ...

Page 15: ...ngs tool bar on your right panel after you restart the program see below The default settings for Print settings Filament and Printer are all currently set as Simple Mode when you first set up your bioprinter We do not recommend using this config file to print any model at this point Each material that you will use for the r3bEL mini bioprinter will require its own unique set of parameter settings...

Page 16: ... file type that you extracted previously Follow steps below for installation 1 Go to File Load Config 2 Select the Lotion ini file and click Open WARNING Do not use the default configuration settings in Slic3r The default settings will cause the extruder to heat to high temperatures and melt your syringe Ensure that you have loaded the lotion ini config file prior to your first print ...

Page 17: ... lotion settings in each Slic3r tab so that it becomes the default by clicking the save button in the upper left corner in each of the settings tabs As a final check to ensure that your temperature settings on Slic3r is correct click on the Filament Settings Tab Make sure you are looking at the lotion config file and check that your temperature settings match the settings shown below ...

Page 18: ...eature and function as listed below A Port Selection B X Y Z Control Panel C Heater Control Note this feature is not used on the r3bEL mini D Extruder Control E Custom Button Area F Command Line G Command Window 6 To connect the bioprinter to Pronterface select the proper COM port This will be whatever port your computer assigns the device to In macOS ports may have a different naming designation ...

Page 19: ...s the bed Note The motion of the bed is relative to the extruder head Y will move the bed to the front relative to the head so the extruder head will shift toward the back of the bed and Y will move the bed to the back relative to the head so the extruder head will shift toward the front of the bed Z coordinate The Z motion moves the bed up and down Use the control bar on the right to move the pla...

Page 20: ...y enter codes every single time in the command line Proceed to create these custom buttons for r3bEL mini To add a button click on the box in the custom button area you will find this at the bottom of the yellow gridded print area shown below A text box will appear fill in each setting accordingly to create customized buttons on your Pronterface software ROW 1 Button title Set Home Button title Se...

Page 21: ...1 E150 F3000 Command G1 E50 F3000 Color 33cc33 Color 5cd65c Button title Extrude 5 mm Button title Extrude 1 mm Command G1 E5 F3000 Command G1 E1 F3000 Color 85e085 Color c2f0c2 ROW 4 Button title Retract 150 mm Button title Retract 50 mm Command G1 E 150 F3000 Command G1 E 50 F3000 Color ff4d4d Color ff6666 Button title Retract 5 mm Button title Retract 1 mm Command G1 E 5 F3000 Command G1 E 1 F3...

Page 22: ...0 is at 48 52 P1 is at 48 52 P2 is at 48 52 and P3 is at 48 52 Extrude 150 mm This button moves the extruder plunger down by 150 mm in distance extruding whatever liquid or material is in the syringe Extrude 50 mm This button moves the extruder plunger down by 50 mm in distance extruding whatever liquid or material is in the syringe Extrude 5 mm This button moves the extruder plunger down by 5 mm ...

Page 23: ...w the steps below omit this step if your computer runs on operating system other than Windows 1 To download the Duet driver files go to the Startup guide r3bEL mini scroll to the Programs to download section and download the Duet Driver file Unzip the two files duet inf and duetinf cat to a local folder on your PC 2 Connect the Duet to your PC using the USB cable 3 Open Device Manager Find the Por...

Page 24: ...t click on the Device and select Update Driver Software as shown below 5 Click on Browse my computer for driver software option and choose the folder where you extracted the files from the DuetWiFi driver zip folder and click on Next ...

Page 25: ...p up window click on Install and wait until it says Windows successfully updated the driver software and click on close 7 Once you close the window you should see that the Unknown device should show up as DuetWifi or Duet Ethernet 3D Printer control electronics COM ...

Page 26: ...structions 5 2 1 YAT Terminal Windows YAT terminal software is used to configure the Wi Fi module on the bioprinter If you are running a Windows operating system follow the steps below 1 Download the Terminal Emulator program YAT from the link https sourceforge net projects y a terminal and install the software 2 Make sure that the bioprinter is connected to the computer using the USB Cable 3 Open...

Page 27: ...r3bEL mini User Manual 27 4 You will now be able to send commands to the board by typing it in the Send Text command box Send the following command M552 Wait until you get the Wi Fi module is idle reply ...

Page 28: ...ters M587 S Name of the Wifi you want to connect to P Password for the network Note The Wi Fi module operates at 2 4 Ghz not 5 Ghz Make sure you enter the details for the 2 4 Ghz network The Name and Password for the network are case sensitive so make sure that you enter the details carefully ...

Page 29: ...at the network you entered has been stored send M587 This command will display a list of networks it has been connected the last one is the most recent network You can set up connection to additional access points in the same way if you wish ...

Page 30: ...After a few seconds it should confirm the connection and give you an IP address If you have configured multiple access points and more than one is in range the bioprinter will connect to the strongest one Note Remember to take down this IP address since you will need it to connect to the printer ...

Page 31: ...tract the contents to a local folder If you get an error can t be opened because it is from an unidentified developer when you try to open the software go to Settings Security Privacy and you should have an option to allow all apps this will make it a trusted app 2 Make sure that the bioprinter is connected to the computer using the USB Cable 3 Open the program and click on options Select the drop...

Page 32: ...r3bEL mini User Manual 32 4 Choose the Terminal section and under Terminal Mode choose the Line mode and click on OK ...

Page 33: ...r3bEL mini User Manual 33 5 Click on Connect in the main window and send the following command and wait till you get the output Wifi module is idle M552 ...

Page 34: ...eters M587 S Name of the Wi Fi you want to connect to P Password for the network Note The Wi Fi module operates at 2 4 Ghz not 5 Ghz Make sure you enter the details for the 2 4 Ghz network The Name and Password for the network are case sensitive make sure that you enter the details carefully ...

Page 35: ...at the network you entered has been stored send M587 This command will display a list of networks it has been connected the last one is the most recent network You can set up connection to additional access points in the same way if you wish ...

Page 36: ...After a few seconds it should confirm the connection and give you an IP address If you have configured multiple access points and more than one is in range the bioprinter will connect to the strongest one Note Remember to take down this IP address since you will need it to connect to the printer ...

Page 37: ...password enter se3d and hit enter The interface will look like 1 Familiarize yourself with the Web control options a Machine control Enables you to control the Printer and extruder movements b Print Status Shows the status of the file being printed Enables you to pause resume your prints c G code console d G code files The g code files uploaded in the SD card e Macros User defined custom macros 2 ...

Page 38: ...er move the extruder higher or lower by clicking on extrude or retract accordingly until the slot in the extruder aligns with the syringe plunger Then slowly push the syringe in until it locks into position on the metal holder 2 After the syringe is in place extrude in 5 or 1 mm increments until liquid is pushed out of the needle tip This is referred to as priming the syringe Wipe off any excess l...

Page 39: ...hich will help ensure print consistency Manually extrude in small increments using the Extrude 5 mm or Extrude 1 mm button until lotion is pushed out of the needle tip Wipe excess lotion away as necessary from the needle tip 6 Set the Z0 position using the Set Z0 custom button Manually move the bioprinter bed using the Z axis control buttons until it is almost in contact with but not touching the ...

Page 40: ...our own files simply use the Load File button as shown in this example 9 Click the Print button to start your print in Pronterface The print should look similar to the picture below Note If the extruder or bed hits the Needle tip Petri dish Well plate or rams into the sides of the printer turn off the main power switch in the bioprinter ...

Page 41: ...ioprinter immediately for any of the following reasons Extruder is repeatedly ramming into sides of printer Extruding excessive amount of liquid Extruding or retracting past the limits of the extrusion unit Extrusion unit is moving past its limits hitting sides of printer Heater malfunction too hot burnt smell etc Wiring malfunction short circuit burnt smell etc Any other situation that could harm...

Page 42: ...ace and have your microSD card adapter ready 1 Home all axes by clicking the Home All button in Pronterface 2 In the G code console command line type the following command G92 X0 Y0 Z0 This will manually set this position to 0 0 0 but will not return a response in the command window Proceed to next step to check that it was successful 3 Press the Get Position button to check the position This shou...

Page 43: ...eight ii The bottom line is used to adjust the X and Y coordinates b For the Z height subtract the value you recorded in step 5 from 0 and add it to the existing value For example if you have 0 9 listed above and the value in the file is Z46 you will subtract 0 9 from 0 which is 0 9 and add it to Z46 and the new value is Z46 9 c For the X and Y positions subtract the value you recorded in step 5 f...

Page 44: ...the extruder to the first probe point d Manually decrease the Z height Z until the syringe tip is almost touching the bed same needle height as at P0 e Click the Get Position button and record the Z height for P1 f Increase the Z height 3mm press the P2 button to move to P2 and lower the Z height until the syringe tip reaches the same height as the other two points Click Get Position and record th...

Page 45: ...u can spray it with 70 ethanol or autoclave the acrylic bed templates 10 Advanced Settings 10 1 G code Adjustments G code is a programming language that allows your computer to communicate with your 3D printer it provides the instructions to tell the 3D printer what to do and how to move This section outlines the relevant commands used to control the r3bEL mini bioprinter A full reference guide fo...

Page 46: ... All coordinates from now on are relative to the last position G92 Set Position Usage G92 Xnnn Ynnn Znnn Ennn Parameters This command can be used without any additional parameters Xnnn new X axis position Ynnn new Y axis position Znnn new Z axis position Example G92 X0 Z0 Set X position to 0 and Z height to 0 10 2 G code Customization For Array Printing To customize your own G code files for new m...

Page 47: ...hose described below follow the instructions to troubleshoot and modify your protocol Drop not releasing from needle tip This indicates that there isn t enough liquid is being pushed out of the syringe This can be fixed by increasing the E distance in the G1 command Two drops releasing from needle tip This problem can happen for two reasons Problem Solution The previous drop did not release See is...

Page 48: ...r computer is connected to the controller board check the red light behind the Micro USB connector is lit up The Pronterface application should also display the following Almost all connection problems can be solved by following these steps in order 1 Turn off printer 2 Unplug USB cable from computer 3 Close Pronterface application 4 Plug USB cable into printer and computer 5 Open Pronterface appl...

Page 49: ...and line and press send Droplet skidding across Petri dish after it is printed If you observe water droplets skidding across the petri dish after it is being printed first check and see if the table surface where the printer is sitting on is flat Next check to make sure that the table is stable We highly recommend that the printer is placed on a fixed table and not one on wheels or movable Misprin...

Page 50: ...nd send it Macro file homeall g not found This usually happened when the SD card in the board is little loose or is not recognized properly To fix this issue 1 Turn off the printer 2 Disconnect the printer in Pronterface Web control 3 Unplug the USB cable from the computer 4 Take the SD card out from the slot in the board and put it back in 5 Plug USB cable back into computer 6 Open Pronterface We...

Page 51: ...5 mL standard disposable syringes 10 mL standard disposable syringes Needle tips 14 32 gauge with Luer lock Minimum volume 1 0 μL CV 5 at 5 0 μL Viscosity range 1 200 000 cP Step resolutions 10 microns OPERATING ENVIRONMENT Power requirements 110 240V AC 1 5A 50 60 Hz SOFTWARE Printer control software Pronterface or Duet web control Operating system Windows or macOS Connectivity microUSB or Wi Fi ...

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